data_15768 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone Resonance Assignments of the N-terminal half of hepatitis core protein ; _BMRB_accession_number 15768 _BMRB_flat_file_name bmr15768.str _Entry_type original _Submission_date 2008-05-12 _Accession_date 2008-05-12 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Duvignaud Jean-Baptiste . . 2 Savard Christian M. . 3 Fromentin Remi . . 4 Majeau Nathalie . . 5 Leclerc Denis . . 6 Gagne Stephane . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 48 "13C chemical shifts" 157 "15N chemical shifts" 48 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2009-04-03 original author . stop_ loop_ _Related_BMRB_accession_number _Relationship 16767 'chemical shifts in 90% H2O/10% D2O' stop_ _Original_release_date 2009-04-03 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Structure and dynamics of the N-terminal half of hepatitis C virus core protein: an intrinsically unstructured protein' _Citation_status submitted _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Duvignaud Jean-Baptiste . . 2 Savard Christian . . 3 Fromentin Remi . . 4 Majeau Nathalie . . 5 Leclerc Denis . . 6 Gagne Stephane M. . stop_ _Journal_abbreviation 'Biochem. Cell Biol.' _Journal_volume . _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year . _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'HCV-C82 monomer' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'N-terminal half' $HCV_core_protein stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_HCV_core_protein _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common HCV_core_protein _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 88 _Mol_residue_sequence ; MSTNPKPQRKTKRNTNRRPQ DVKFPGGGQIVGGVYLLPRR GPRLGVRATRKTSERSQPRG RRQPIPKARRPEGRTWAQPG YPHHHHHH ; loop_ _Residue_seq_code _Residue_label 1 MET 2 SER 3 THR 4 ASN 5 PRO 6 LYS 7 PRO 8 GLN 9 ARG 10 LYS 11 THR 12 LYS 13 ARG 14 ASN 15 THR 16 ASN 17 ARG 18 ARG 19 PRO 20 GLN 21 ASP 22 VAL 23 LYS 24 PHE 25 PRO 26 GLY 27 GLY 28 GLY 29 GLN 30 ILE 31 VAL 32 GLY 33 GLY 34 VAL 35 TYR 36 LEU 37 LEU 38 PRO 39 ARG 40 ARG 41 GLY 42 PRO 43 ARG 44 LEU 45 GLY 46 VAL 47 ARG 48 ALA 49 THR 50 ARG 51 LYS 52 THR 53 SER 54 GLU 55 ARG 56 SER 57 GLN 58 PRO 59 ARG 60 GLY 61 ARG 62 ARG 63 GLN 64 PRO 65 ILE 66 PRO 67 LYS 68 ALA 69 ARG 70 ARG 71 PRO 72 GLU 73 GLY 74 ARG 75 THR 76 TRP 77 ALA 78 GLN 79 PRO 80 GLY 81 TYR 82 PRO 83 HIS 84 HIS 85 HIS 86 HIS 87 HIS 88 HIS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-06-13 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 15767 HCV_core_protein 100.00 88 100.00 100.00 1.16e-53 BMRB 4669 "HEPATITIS C VIRUS CAPSID PROTEIN" 50.00 44 100.00 100.00 1.54e-20 PDB 1CWX "Solution Structure Of The Hepatitis C Virus N-Terminal Capsid Protein 2-45 [c-Hcv(2-45)]" 50.00 44 100.00 100.00 1.54e-20 PDB 1XCQ "Complex Hcv Core-Fab 19d9d6-Protein L Mutant (D55a,L57h,Y64w) In Space Group P21" 50.00 44 100.00 100.00 1.54e-20 PDB 1XCT "Complex Hcv Core-Fab 19d9d6-Protein L Mutant (D55a, L57h, Y64w) In Space Group P21212" 50.00 44 100.00 100.00 1.54e-20 PDB 1XF5 "Complex Hcv Core-Fab 19d9d6-Protein L Mutant (H74c, Y64w)in Space Group P21212" 50.00 44 100.00 100.00 1.54e-20 DBJ BAA00452 "polyprotein, partial [Hepatitis C virus]" 93.18 441 100.00 100.00 2.65e-47 DBJ BAA00705 "structural protein, partial [Hepatitis C virus]" 93.18 513 97.56 97.56 1.52e-44 DBJ BAA00706 "structural protein, partial [Hepatitis C virus]" 93.18 513 97.56 97.56 3.70e-45 DBJ BAA00968 "structural protein, partial [Hepatitis C virus]" 93.18 513 98.78 98.78 5.14e-46 DBJ BAA01529 "polyprotein, partial [Hepatitis C virus]" 93.18 520 97.56 97.56 5.42e-45 EMBL CAA03854 "polyprotein [Hepatitis C virus]" 93.18 3010 97.56 97.56 2.02e-44 EMBL CAA43788 "polyprotein, partial [Hepatitis C virus]" 93.18 783 97.56 98.78 2.97e-45 EMBL CAA43790 "JK2, partial [Hepatitis C virus]" 93.18 783 97.56 98.78 3.60e-45 EMBL CAA43793 "JK1-full [Hepatitis C virus]" 93.18 3010 97.56 98.78 1.21e-44 EMBL CAA46717 "unnamed protein product, partial [Hepatitis C virus]" 93.18 369 97.56 98.78 7.80e-46 GB AAA16300 "core envelope protein, partial [Hepatitis C virus]" 93.18 430 98.78 98.78 1.04e-46 GB AAA16311 "core protein, partial [Hepatitis C virus]" 93.18 83 97.56 100.00 3.68e-47 GB AAA20153 "structural region, partial [Hepatitis C virus]" 93.18 411 100.00 100.00 7.66e-47 GB AAA20154 "structural region, partial [Hepatitis C virus]" 93.18 411 97.56 98.78 2.31e-46 GB AAA21040 "polyprotein, partial [Hepatitis C virus]" 93.18 191 100.00 100.00 1.12e-47 PIR JQ1926 "polyprotein - hepatitis C virus (isolate HCV-476) [Hepatitis C virus]" 93.18 523 97.56 97.56 7.29e-45 REF NP_671491 "polyprotein [Hepatitis C virus]" 93.18 3011 100.00 100.00 1.22e-45 REF YP_001469632 "HCV polyprotein [Hepatitis C virus genotype 4]" 93.18 3008 97.56 98.78 1.29e-44 SP O39929 "RecName: Full=Genome polyprotein; Contains: RecName: Full=Core protein p21; AltName: Full=Capsid protein C; AltName: Full=p21; " 93.18 3008 97.56 98.78 1.29e-44 SP O92972 "RecName: Full=Genome polyprotein; Contains: RecName: Full=Core protein p21; AltName: Full=Capsid protein C; AltName: Full=p21; " 93.18 3010 97.56 97.56 1.83e-44 SP P26662 "RecName: Full=Genome polyprotein; Contains: RecName: Full=Core protein p21; AltName: Full=Capsid protein C; AltName: Full=p21; " 93.18 3010 100.00 100.00 9.12e-46 SP P26663 "RecName: Full=Genome polyprotein; Contains: RecName: Full=Core protein p21; AltName: Full=Capsid protein C; AltName: Full=p21; " 93.18 3010 98.78 98.78 1.37e-44 SP P26664 "RecName: Full=Genome polyprotein; Contains: RecName: Full=Core protein p21; AltName: Full=Capsid protein C; AltName: Full=p21; " 93.18 3011 97.56 98.78 1.29e-44 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $HCV_core_protein 'Hepatitis C virus' 11103 Viruses . Hepacivirus 'Hepatitis C virus' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $HCV_core_protein 'recombinant technology' . Escherichia coli . pet3D stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details ; 90% TFE 10% H2O 0.2mM DSS 0.1% NaN3 1x inhibitor cocktail pH 6.6 ; loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $HCV_core_protein 0.4 mM '[U-100% 13C; U-100% 15N]' TFE 90 % . H2O 10 % . DSS 0.2 mM . NaN3 0.1 % . stop_ save_ ############################ # Computer software used # ############################ save_NMRView _Saveframe_category software _Name NMRView _Version 5.2.2.01 loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCO_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.6 . pH pressure 1 . atm temperature 278 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 . indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 . indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCO' '3D HNCACB' '3D CBCA(CO)NH' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'N-terminal half' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 SER C C 176.38 0.23 1 2 2 2 SER CA C 59.64 0.23 1 3 2 2 SER CB C 64.13 0.23 1 4 3 3 THR H H 7.87 0.02 1 5 3 3 THR C C 174.04 0.23 1 6 3 3 THR CA C 61.21 0.23 1 7 3 3 THR CB C 69.29 0.23 1 8 3 3 THR N N 110.89 0.16 1 9 4 4 ASN H H 8.11 0.02 1 10 4 4 ASN CA C 51.33 0.23 1 11 4 4 ASN CB C 38.11 0.23 1 12 4 4 ASN N N 120.72 0.16 1 13 5 5 PRO C C 176.16 0.23 1 14 5 5 PRO CA C 63.42 0.23 1 15 5 5 PRO CB C 31.81 0.23 1 16 6 6 LYS H H 8.08 0.02 1 17 6 6 LYS CA C 54.54 0.23 1 18 6 6 LYS CB C 32.33 0.23 1 19 6 6 LYS N N 120.37 0.16 1 20 9 9 ARG C C 176.69 0.23 1 21 9 9 ARG CA C 57.05 0.23 1 22 9 9 ARG CB C 30.54 0.23 1 23 10 10 LYS H H 8.18 0.02 1 24 10 10 LYS C C 176.80 0.23 1 25 10 10 LYS CA C 57.25 0.23 1 26 10 10 LYS CB C 32.69 0.23 1 27 10 10 LYS N N 120.58 0.16 1 28 11 11 THR H H 8.01 0.02 1 29 11 11 THR C C 175.01 0.23 1 30 11 11 THR CA C 62.28 0.23 1 31 11 11 THR CB C 70.31 0.23 1 32 11 11 THR N N 112.70 0.16 1 33 12 12 LYS H H 8.13 0.02 1 34 12 12 LYS C C 177.00 0.23 1 35 12 12 LYS CA C 57.30 0.23 1 36 12 12 LYS CB C 32.53 0.23 1 37 12 12 LYS N N 121.89 0.16 1 38 13 13 ARG H H 8.15 0.02 1 39 13 13 ARG C C 176.41 0.23 1 40 13 13 ARG CA C 57.04 0.23 1 41 13 13 ARG CB C 30.66 0.23 1 42 13 13 ARG N N 119.63 0.16 1 43 14 14 ASN H H 8.25 0.02 1 44 14 14 ASN C C 175.55 0.23 1 45 14 14 ASN CA C 53.67 0.23 1 46 14 14 ASN CB C 38.52 0.23 1 47 14 14 ASN N N 117.57 0.16 1 48 15 15 THR H H 7.93 0.02 1 49 15 15 THR C C 174.22 0.23 1 50 15 15 THR CA C 62.23 0.23 1 51 15 15 THR CB C 69.77 0.23 1 52 15 15 THR N N 112.16 0.16 1 53 16 16 ASN H H 8.18 0.02 1 54 16 16 ASN C C 174.55 0.23 1 55 16 16 ASN CA C 53.45 0.23 1 56 16 16 ASN CB C 38.68 0.23 1 57 16 16 ASN N N 119.87 0.16 1 58 17 17 ARG H H 7.99 0.02 1 59 17 17 ARG C C 175.38 0.23 1 60 17 17 ARG CA C 55.81 0.23 1 61 17 17 ARG CB C 31.14 0.23 1 62 17 17 ARG N N 119.95 0.16 1 63 18 18 ARG H H 8.45 0.02 1 64 18 18 ARG CA C 53.89 0.23 1 65 18 18 ARG CB C 30.18 0.23 1 66 18 18 ARG N N 121.68 0.16 1 67 19 19 PRO C C 177.46 0.23 1 68 19 19 PRO CA C 64.76 0.23 1 69 19 19 PRO CB C 31.53 0.23 1 70 20 20 GLN H H 8.80 0.02 1 71 20 20 GLN C C 175.80 0.23 1 72 20 20 GLN CA C 57.25 0.23 1 73 20 20 GLN CB C 28.19 0.23 1 74 20 20 GLN N N 115.66 0.16 1 75 21 21 ASP H H 8.07 0.02 1 76 21 21 ASP C C 176.21 0.23 1 77 21 21 ASP CA C 54.86 0.23 1 78 21 21 ASP CB C 41.20 0.23 1 79 21 21 ASP N N 119.56 0.16 1 80 22 22 VAL H H 7.60 0.02 1 81 22 22 VAL C C 175.66 0.23 1 82 22 22 VAL CA C 63.17 0.23 1 83 22 22 VAL CB C 32.15 0.23 1 84 22 22 VAL N N 119.34 0.16 1 85 23 23 LYS H H 7.86 0.02 1 86 23 23 LYS C C 175.26 0.23 1 87 23 23 LYS CA C 55.76 0.23 1 88 23 23 LYS CB C 33.01 0.23 1 89 23 23 LYS N N 122.35 0.16 1 90 24 24 PHE H H 7.88 0.02 1 91 24 24 PHE CA C 55.42 0.23 1 92 24 24 PHE CB C 39.46 0.23 1 93 24 24 PHE N N 119.95 0.16 1 94 25 25 PRO C C 177.11 0.23 1 95 25 25 PRO CA C 63.78 0.23 1 96 25 25 PRO CB C 31.34 0.23 1 97 26 26 GLY H H 7.83 0.02 1 98 26 26 GLY C C 174.92 0.23 1 99 26 26 GLY CA C 45.42 0.23 1 100 26 26 GLY N N 107.84 0.16 1 101 27 27 GLY H H 8.25 0.02 1 102 27 27 GLY C C 174.96 0.23 1 103 27 27 GLY CA C 46.01 0.23 1 104 27 27 GLY N N 107.66 0.16 1 105 28 28 GLY H H 8.32 0.02 1 106 28 28 GLY C C 174.66 0.23 1 107 28 28 GLY CA C 45.85 0.23 1 108 28 28 GLY N N 107.45 0.16 1 109 29 29 GLN H H 8.14 0.02 1 110 29 29 GLN C C 176.57 0.23 1 111 29 29 GLN CA C 57.04 0.23 1 112 29 29 GLN CB C 29.16 0.23 1 113 29 29 GLN N N 118.57 0.16 1 114 30 30 ILE H H 7.85 0.02 1 115 30 30 ILE C C 176.95 0.23 1 116 30 30 ILE CA C 62.66 0.23 1 117 30 30 ILE CB C 38.45 0.23 1 118 30 30 ILE N N 119.77 0.16 1 119 31 31 VAL H H 7.87 0.02 1 120 31 31 VAL C C 177.07 0.23 1 121 31 31 VAL CA C 64.02 0.23 1 122 31 31 VAL CB C 32.21 0.23 1 123 31 31 VAL N N 120.62 0.16 1 124 32 32 GLY H H 8.13 0.02 1 125 32 32 GLY C C 174.87 0.23 1 126 32 32 GLY CA C 45.90 0.23 1 127 32 32 GLY N N 108.30 0.16 1 128 33 33 GLY H H 7.90 0.02 1 129 33 33 GLY C C 174.87 0.23 1 130 33 33 GLY CA C 46.07 0.23 1 131 33 33 GLY N N 106.60 0.16 1 132 34 34 VAL H H 7.76 0.02 1 133 34 34 VAL C C 176.68 0.23 1 134 34 34 VAL CA C 64.29 0.23 1 135 34 34 VAL CB C 32.20 0.23 1 136 34 34 VAL N N 118.21 0.16 1 137 35 35 TYR H H 7.75 0.02 1 138 35 35 TYR C C 176.17 0.23 1 139 35 35 TYR CA C 59.12 0.23 1 140 35 35 TYR CB C 38.36 0.23 1 141 35 35 TYR N N 119.13 0.16 1 142 36 36 LEU H H 7.82 0.02 1 143 36 36 LEU C C 177.29 0.23 1 144 36 36 LEU CA C 55.35 0.23 1 145 36 36 LEU CB C 42.69 0.23 1 146 36 36 LEU N N 118.99 0.16 1 147 37 37 LEU H H 7.62 0.02 1 148 37 37 LEU N N 120.76 0.16 1 149 40 40 ARG C C 175.99 0.23 1 150 40 40 ARG CA C 56.31 0.23 1 151 40 40 ARG CB C 31.06 0.23 1 152 41 41 GLY H H 8.02 0.02 1 153 41 41 GLY CA C 45.00 0.23 1 154 41 41 GLY N N 107.88 0.16 1 155 56 56 SER C C 173.82 0.23 1 156 56 56 SER CA C 58.88 0.23 1 157 56 56 SER CB C 64.06 0.23 1 158 57 57 GLN H H 7.63 0.02 1 159 57 57 GLN CA C 54.06 0.23 1 160 57 57 GLN CB C 28.78 0.23 1 161 57 57 GLN N N 120.83 0.16 1 162 58 58 PRO C C 176.90 0.23 1 163 58 58 PRO CA C 63.73 0.23 1 164 58 58 PRO CB C 31.45 0.23 1 165 59 59 ARG H H 8.16 0.02 1 166 59 59 ARG C C 176.58 0.23 1 167 59 59 ARG CA C 56.29 0.23 1 168 59 59 ARG CB C 30.82 0.23 1 169 59 59 ARG N N 119.49 0.16 1 170 60 60 GLY H H 8.19 0.02 1 171 60 60 GLY C C 173.89 0.23 1 172 60 60 GLY CA C 45.21 0.23 1 173 60 60 GLY N N 107.70 0.16 1 174 61 61 ARG H H 7.92 0.02 1 175 61 61 ARG CA C 56.09 0.23 1 176 61 61 ARG CB C 30.82 0.23 1 177 61 61 ARG N N 119.03 0.16 1 178 64 64 PRO C C 175.74 0.23 1 179 64 64 PRO CA C 62.99 0.23 1 180 64 64 PRO CB C 31.50 0.23 1 181 65 65 ILE H H 7.69 0.02 1 182 65 65 ILE CA C 58.72 0.23 1 183 65 65 ILE CB C 39.24 0.23 1 184 65 65 ILE N N 120.05 0.16 1 185 66 66 PRO C C 176.49 0.23 1 186 66 66 PRO CA C 63.49 0.23 1 187 66 66 PRO CB C 31.64 0.23 1 188 67 67 LYS H H 7.94 0.02 1 189 67 67 LYS C C 176.13 0.23 1 190 67 67 LYS CA C 56.77 0.23 1 191 67 67 LYS CB C 33.12 0.23 1 192 67 67 LYS N N 120.09 0.16 1 193 68 68 ALA H H 7.90 0.02 1 194 68 68 ALA C C 177.18 0.23 1 195 68 68 ALA CA C 52.70 0.23 1 196 68 68 ALA CB C 18.78 0.23 1 197 68 68 ALA N N 122.07 0.16 1 198 69 69 ARG H H 7.90 0.02 1 199 69 69 ARG C C 175.36 0.23 1 200 69 69 ARG CA C 55.38 0.23 1 201 69 69 ARG CB C 30.98 0.23 1 202 69 69 ARG N N 117.82 0.16 1 203 70 70 ARG H H 7.97 0.02 1 204 70 70 ARG CA C 53.87 0.23 1 205 70 70 ARG CB C 30.04 0.23 1 206 70 70 ARG N N 120.87 0.16 1 207 71 71 PRO C C 177.27 0.23 1 208 71 71 PRO CA C 64.37 0.23 1 209 71 71 PRO CB C 31.51 0.23 1 210 72 72 GLU H H 9.18 0.02 1 211 72 72 GLU C C 177.06 0.23 1 212 72 72 GLU CA C 57.79 0.23 1 213 72 72 GLU CB C 29.16 0.23 1 214 72 72 GLU N N 119.41 0.16 1 215 73 73 GLY H H 8.14 0.02 1 216 73 73 GLY C C 174.42 0.23 1 217 73 73 GLY CA C 45.53 0.23 1 218 73 73 GLY N N 107.84 0.16 1 219 74 74 ARG H H 7.92 0.02 1 220 74 74 ARG C C 176.68 0.23 1 221 74 74 ARG CA C 56.40 0.23 1 222 74 74 ARG CB C 30.18 0.23 1 223 74 74 ARG N N 118.89 0.16 1 224 75 75 THR H H 7.85 0.02 1 225 75 75 THR C C 174.45 0.23 1 226 75 75 THR CA C 62.87 0.23 1 227 75 75 THR CB C 69.56 0.23 1 228 75 75 THR N N 112.51 0.16 1 229 76 76 TRP H H 7.49 0.02 1 230 76 76 TRP C C 175.59 0.23 1 231 76 76 TRP CA C 57.14 0.23 1 232 76 76 TRP CB C 29.05 0.23 1 233 76 76 TRP N N 119.91 0.16 1 234 77 77 ALA H H 7.45 0.02 1 235 77 77 ALA C C 176.50 0.23 1 236 77 77 ALA CA C 52.06 0.23 1 237 77 77 ALA CB C 18.40 0.23 1 238 77 77 ALA N N 122.63 0.16 1 239 78 78 GLN H H 7.69 0.02 1 240 78 78 GLN CA C 53.52 0.23 1 241 78 78 GLN CB C 29.16 0.23 1 242 78 78 GLN N N 118.57 0.16 1 243 79 79 PRO C C 176.93 0.23 1 244 79 79 PRO CA C 63.80 0.23 1 245 79 79 PRO CB C 31.53 0.23 1 246 80 80 GLY H H 8.26 0.02 1 247 80 80 GLY C C 173.51 0.23 1 248 80 80 GLY CA C 44.78 0.23 1 249 80 80 GLY N N 107.35 0.16 1 250 81 81 TYR H H 7.85 0.02 1 251 81 81 TYR CA C 57.48 0.23 1 252 81 81 TYR CB C 38.41 0.23 1 253 81 81 TYR N N 120.72 0.16 1 stop_ save_