data_15481 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 15N and 1HN assignments of the EVH1 domain of human HOMER3A ; _BMRB_accession_number 15481 _BMRB_flat_file_name bmr15481.str _Entry_type original _Submission_date 2007-09-20 _Accession_date 2007-09-20 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'EVH1 domain of the human Homer3A protein. N-terminal SM residues are a cloning artefact from the vector' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Higman Victoria A. . 2 Schmieder Peter . . 3 Diehl Anne . . 4 Bishop Shurene . . 5 Oschkinat Hartmut . . 6 Arrowsmith Cheryl H. . 7 Wiegelt Johan . . 8 Edwards Aled . . 9 Sundstrom Michael . . 10 Ball Linda J. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 T1_relaxation 1 T2_relaxation 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 114 "15N chemical shifts" 114 "T1 relaxation values" 91 "T2 relaxation values" 91 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2007-09-27 original author . stop_ _Original_release_date 2007-09-27 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title '15N and 1HN assignments of the EVH1 domain of human HOMER3A' _Citation_status 'in preparation' _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Higman Victoria A. . 2 Schmieder Peter . . 3 Bishop Shurene . . 4 Diehl Anne . . 5 Oschkinat Hartmut . . 6 Arrowsmith Cheryl H. . 7 Edwards Aled . . 8 Sundstrom Michael . . 9 Wiegelt Johan . . 10 Ball Linda J. . stop_ _Journal_abbreviation 'Biomol. NMR Assignments' _Journal_volume . _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year . _Details . loop_ _Keyword 'EVH1 domain' HOMER 'metabotropic glutamate receptor binding' neuronal 'synaptic signalling protein' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'HOMER3A EVH1 domain' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'HOMER3A EVH1 domain' $HOMER3A_EVH1_domain stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_HOMER3A_EVH1_domain _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common HOMER3A_EVH1_domain _Molecular_mass . _Mol_thiol_state 'not present' loop_ _Biological_function 'neuronal signalling protein' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 117 _Mol_residue_sequence ; SMAREQPIFSTRAHVFQIDP ATKRNWIPAGKHALTVSYFY DATRNVYRIISIGGAKAIIN STVTPNMTFTKTSQKFGQWA DSRANTVYGLGFASEQHLTQ FAEKFQEVKEAARLARE ; loop_ _Residue_seq_code _Residue_label 1 SER 2 MET 3 ALA 4 ARG 5 GLU 6 GLN 7 PRO 8 ILE 9 PHE 10 SER 11 THR 12 ARG 13 ALA 14 HIS 15 VAL 16 PHE 17 GLN 18 ILE 19 ASP 20 PRO 21 ALA 22 THR 23 LYS 24 ARG 25 ASN 26 TRP 27 ILE 28 PRO 29 ALA 30 GLY 31 LYS 32 HIS 33 ALA 34 LEU 35 THR 36 VAL 37 SER 38 TYR 39 PHE 40 TYR 41 ASP 42 ALA 43 THR 44 ARG 45 ASN 46 VAL 47 TYR 48 ARG 49 ILE 50 ILE 51 SER 52 ILE 53 GLY 54 GLY 55 ALA 56 LYS 57 ALA 58 ILE 59 ILE 60 ASN 61 SER 62 THR 63 VAL 64 THR 65 PRO 66 ASN 67 MET 68 THR 69 PHE 70 THR 71 LYS 72 THR 73 SER 74 GLN 75 LYS 76 PHE 77 GLY 78 GLN 79 TRP 80 ALA 81 ASP 82 SER 83 ARG 84 ALA 85 ASN 86 THR 87 VAL 88 TYR 89 GLY 90 LEU 91 GLY 92 PHE 93 ALA 94 SER 95 GLU 96 GLN 97 HIS 98 LEU 99 THR 100 GLN 101 PHE 102 ALA 103 GLU 104 LYS 105 PHE 106 GLN 107 GLU 108 VAL 109 LYS 110 GLU 111 ALA 112 ALA 113 ARG 114 LEU 115 ALA 116 ARG 117 GLU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-08 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 2P8V "Crystal Structure Of Human Homer3 Evh1 Domain" 100.00 117 99.15 99.15 3.77e-79 DBJ BAA35110 "Vesl-3 [Rattus norvegicus]" 100.00 358 97.44 97.44 8.08e-77 DBJ BAG36707 "unnamed protein product [Homo sapiens]" 100.00 361 99.15 99.15 2.11e-78 DBJ BAG54040 "unnamed protein product [Homo sapiens]" 100.00 361 99.15 99.15 2.11e-78 EMBL CAB75536 "HOMER-3A (flip) protein [Homo sapiens]" 100.00 361 99.15 99.15 2.11e-78 EMBL CAB75543 "Homer-3B protein [Homo sapiens]" 86.32 325 98.02 98.02 2.22e-66 EMBL CAB75544 "Homer-3C protein [Homo sapiens]" 100.00 145 99.15 99.15 9.11e-79 EMBL CAB75545 "Homer-3D protein [Homo sapiens]" 100.00 121 98.29 99.15 1.14e-78 EMBL CAC35223 "GLGF-domain protein (Homer-3/Vesl-3) [Chlorocebus aethiops]" 77.78 91 100.00 100.00 5.36e-60 GB AAB81545 "R27090_3 [Homo sapiens]" 100.00 374 99.15 99.15 5.96e-78 GB AAC71025 "homer-3 [Mus musculus]" 100.00 358 97.44 97.44 1.25e-76 GB AAC71029 "homer-3 [Homo sapiens]" 100.00 358 99.15 99.15 1.86e-78 GB AAH05773 "Homer homolog 3 (Drosophila) [Mus musculus]" 100.00 356 97.44 97.44 1.28e-76 GB AAH12113 "Homer homolog 3 (Drosophila) [Homo sapiens]" 100.00 361 99.15 99.15 2.11e-78 REF NP_001139193 "homer protein homolog 3 isoform 2 [Homo sapiens]" 100.00 358 99.15 99.15 1.86e-78 REF NP_001139194 "homer protein homolog 3 isoform 1 [Homo sapiens]" 100.00 361 99.15 99.15 2.28e-78 REF NP_001139196 "homer protein homolog 3 isoform 3 [Homo sapiens]" 86.32 325 98.02 98.02 2.17e-66 REF NP_001139625 "homer protein homolog 3 isoform 1 [Mus musculus]" 100.00 359 97.44 97.44 1.19e-76 REF NP_001244618 "homer protein homolog 3 [Macaca mulatta]" 100.00 361 99.15 99.15 2.23e-78 SP Q99JP6 "RecName: Full=Homer protein homolog 3; Short=Homer-3" 100.00 356 97.44 97.44 1.56e-76 SP Q9NSC5 "RecName: Full=Homer protein homolog 3; Short=Homer-3" 100.00 361 99.15 99.15 2.28e-78 SP Q9Z2X5 "RecName: Full=Homer protein homolog 3; Short=Homer-3; AltName: Full=VASP/Ena-related gene up-regulated during seizure and LTP 3" 100.00 358 97.44 97.44 8.08e-77 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $HOMER3A_EVH1_domain Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name _Details $HOMER3A_EVH1_domain 'recombinant technology' . Escherichia coli 'Rosetta BL21(DE3)' pNIC28-Bsa4 'The vector adds an N-terminal hexahistidine purification tag with a TEV-protease cleavage site and confers kanamycin resistance to cells.' stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details 'EVH1 domain of human HOMER3A' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $HOMER3A_EVH1_domain 0.7 mM '[U-99% 15N]' 'potassium phosphate' 20 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' 'sodium azide' 0.15 mM 'natural abundance' DTT 1 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_xwinnmr _Saveframe_category software _Name xwinnmr _Version 3.5 loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' 'Bruker BioSpin GmbH, Rheinstetten, Germany' . stop_ loop_ _Task collection stop_ _Details . save_ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version 1.3 loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task processing stop_ _Details . save_ save_CcpNmr_Analysis _Saveframe_category software _Name ANALYSIS _Version 1.0.15 loop_ _Vendor _Address _Electronic_address CCPNMR ; Cambridge, UK. Vranken et al. Proteins 59, 687-696 ; http://www.ccpn.ac.uk/ stop_ loop_ _Task 'chemical shift assignment' 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 600 _Details 'Standard configuration with triple resonance probe equipped with self-shielded triple axis gradient coils' save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_1H-15N_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ save_3D_1H-15N_TOCSY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N TOCSY' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_-_T1_series_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC - T1 series' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_-_T2_series_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC - T2 series' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.0 . pH pressure 1 . atm temperature 297 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.00 external direct cylindrical 'separate tube (no insert) similar to the experimental sample tube' parallel 1.000000000 DSS N 15 'methyl protons' ppm 0.00 external indirect cylindrical 'separate tube (no insert) similar to the experimental sample tube' parallel 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $CcpNmr_Analysis stop_ loop_ _Experiment_label '2D 1H-15N HSQC' '3D 1H-15N NOESY' '3D 1H-15N TOCSY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'HOMER3A EVH1 domain' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 MET H H 8.175 0.01 1 2 2 2 MET N N 116.778 0.1 1 3 3 3 ALA H H 8.408 0.01 1 4 3 3 ALA N N 125.898 0.1 1 5 4 4 ARG H H 8.339 0.01 1 6 4 4 ARG N N 120.307 0.1 1 7 5 5 GLU H H 8.345 0.01 1 8 5 5 GLU N N 121.351 0.1 1 9 6 6 GLN H H 8.577 0.01 1 10 6 6 GLN N N 121.831 0.1 1 11 8 8 ILE H H 8.551 0.01 1 12 8 8 ILE N N 117.054 0.1 1 13 9 9 PHE H H 7.264 0.01 1 14 9 9 PHE N N 119.611 0.1 1 15 10 10 SER H H 7.882 0.01 1 16 10 10 SER N N 123.306 0.1 1 17 11 11 THR H H 8.876 0.01 1 18 11 11 THR N N 118.480 0.1 1 19 12 12 ARG H H 8.170 0.01 1 20 12 12 ARG N N 122.883 0.1 1 21 13 13 ALA H H 8.964 0.01 1 22 13 13 ALA N N 123.291 0.1 1 23 14 14 HIS H H 8.873 0.01 1 24 14 14 HIS N N 120.815 0.1 1 25 15 15 VAL H H 8.457 0.01 1 26 15 15 VAL N N 124.783 0.1 1 27 16 16 PHE H H 9.407 0.01 1 28 16 16 PHE N N 125.496 0.1 1 29 17 17 GLN H H 8.899 0.01 1 30 17 17 GLN N N 118.259 0.1 1 31 18 18 ILE H H 8.048 0.01 1 32 18 18 ILE N N 126.060 0.1 1 33 19 19 ASP H H 8.385 0.01 1 34 19 19 ASP N N 127.599 0.1 1 35 21 21 ALA H H 8.100 0.01 1 36 21 21 ALA N N 119.788 0.1 1 37 22 22 THR H H 7.699 0.01 1 38 22 22 THR N N 106.540 0.1 1 39 23 23 LYS H H 8.249 0.01 1 40 23 23 LYS N N 117.679 0.1 1 41 24 24 ARG H H 7.895 0.01 1 42 24 24 ARG N N 115.494 0.1 1 43 25 25 ASN H H 7.569 0.01 1 44 25 25 ASN N N 116.303 0.1 1 45 26 26 TRP H H 8.567 0.01 1 46 26 26 TRP HE1 H 9.684 0.01 1 47 26 26 TRP N N 121.191 0.1 1 48 26 26 TRP NE1 N 128.472 0.1 1 49 27 27 ILE H H 9.708 0.01 1 50 27 27 ILE N N 125.873 0.1 1 51 29 29 ALA H H 8.597 0.01 1 52 29 29 ALA N N 125.992 0.1 1 53 30 30 GLY H H 7.517 0.01 1 54 30 30 GLY N N 105.088 0.1 1 55 31 31 LYS H H 8.406 0.01 1 56 31 31 LYS N N 120.740 0.1 1 57 32 32 HIS H H 7.755 0.01 1 58 32 32 HIS N N 115.798 0.1 1 59 33 33 ALA H H 8.590 0.01 1 60 33 33 ALA N N 124.138 0.1 1 61 34 34 LEU H H 8.346 0.01 1 62 34 34 LEU N N 122.176 0.1 1 63 35 35 THR H H 8.490 0.01 1 64 35 35 THR N N 116.909 0.1 1 65 36 36 VAL H H 9.114 0.01 1 66 36 36 VAL N N 131.270 0.1 1 67 37 37 SER H H 8.796 0.01 1 68 37 37 SER N N 120.876 0.1 1 69 38 38 TYR H H 8.748 0.01 1 70 38 38 TYR N N 119.743 0.1 1 71 39 39 PHE H H 9.796 0.01 1 72 39 39 PHE N N 120.964 0.1 1 73 40 40 TYR H H 9.158 0.01 1 74 40 40 TYR N N 121.675 0.1 1 75 41 41 ASP H H 8.406 0.01 1 76 41 41 ASP N N 128.859 0.1 1 77 42 42 ALA H H 8.346 0.01 1 78 42 42 ALA N N 127.160 0.1 1 79 43 43 THR H H 8.171 0.01 1 80 43 43 THR N N 113.215 0.1 1 81 44 44 ARG H H 7.089 0.01 1 82 44 44 ARG N N 118.574 0.1 1 83 45 45 ASN H H 8.035 0.01 1 84 45 45 ASN N N 118.750 0.1 1 85 46 46 VAL H H 7.071 0.01 1 86 46 46 VAL N N 110.693 0.1 1 87 47 47 TYR H H 9.570 0.01 1 88 47 47 TYR N N 122.857 0.1 1 89 48 48 ARG H H 9.199 0.01 1 90 48 48 ARG N N 118.795 0.1 1 91 49 49 ILE H H 9.103 0.01 1 92 49 49 ILE N N 122.929 0.1 1 93 50 50 ILE H H 9.095 0.01 1 94 50 50 ILE N N 124.905 0.1 1 95 51 51 SER H H 8.528 0.01 1 96 51 51 SER N N 120.673 0.1 1 97 52 52 ILE H H 9.188 0.01 1 98 52 52 ILE N N 128.275 0.1 1 99 53 53 GLY H H 8.613 0.01 1 100 53 53 GLY N N 114.671 0.1 1 101 54 54 GLY H H 8.673 0.01 1 102 54 54 GLY N N 113.388 0.1 1 103 55 55 ALA H H 8.674 0.01 1 104 55 55 ALA N N 123.916 0.1 1 105 56 56 LYS H H 7.762 0.01 1 106 56 56 LYS N N 117.378 0.1 1 107 57 57 ALA H H 8.580 0.01 1 108 57 57 ALA N N 125.598 0.1 1 109 58 58 ILE H H 8.338 0.01 1 110 58 58 ILE N N 125.492 0.1 1 111 59 59 ILE H H 7.770 0.01 1 112 59 59 ILE N N 116.695 0.1 1 113 60 60 ASN H H 7.838 0.01 1 114 60 60 ASN N N 125.298 0.1 1 115 61 61 SER H H 9.041 0.01 1 116 61 61 SER N N 118.959 0.1 1 117 62 62 THR H H 9.121 0.01 1 118 62 62 THR N N 126.262 0.1 1 119 63 63 VAL H H 8.667 0.01 1 120 63 63 VAL N N 127.936 0.1 1 121 64 64 THR H H 7.974 0.01 1 122 64 64 THR N N 119.434 0.1 1 123 66 66 ASN H H 7.985 0.01 1 124 66 66 ASN N N 111.553 0.1 1 125 67 67 MET H H 7.570 0.01 1 126 67 67 MET N N 121.580 0.1 1 127 68 68 THR H H 8.811 0.01 1 128 68 68 THR N N 115.704 0.1 1 129 69 69 PHE H H 8.600 0.01 1 130 69 69 PHE N N 128.954 0.1 1 131 70 70 THR H H 8.814 0.01 1 132 70 70 THR N N 124.844 0.1 1 133 71 71 LYS H H 8.795 0.01 1 134 71 71 LYS N N 127.001 0.1 1 135 72 72 THR H H 8.677 0.01 1 136 72 72 THR N N 120.797 0.1 1 137 73 73 SER H H 8.547 0.01 1 138 73 73 SER N N 116.341 0.1 1 139 74 74 GLN H H 8.145 0.01 1 140 74 74 GLN N N 120.601 0.1 1 141 75 75 LYS H H 7.815 0.01 1 142 75 75 LYS N N 118.324 0.1 1 143 76 76 PHE H H 7.537 0.01 1 144 76 76 PHE N N 124.053 0.1 1 145 77 77 GLY H H 8.110 0.01 1 146 77 77 GLY N N 112.721 0.1 1 147 78 78 GLN H H 8.078 0.01 1 148 78 78 GLN N N 113.595 0.1 1 149 79 79 TRP H H 9.050 0.01 1 150 79 79 TRP HE1 H 10.143 0.01 1 151 79 79 TRP N N 119.657 0.1 1 152 79 79 TRP NE1 N 126.519 0.1 1 153 80 80 ALA H H 8.677 0.01 1 154 80 80 ALA N N 123.040 0.1 1 155 81 81 ASP H H 8.409 0.01 1 156 81 81 ASP N N 121.936 0.1 1 157 82 82 SER H H 9.051 0.01 1 158 82 82 SER N N 121.325 0.1 1 159 83 83 ARG H H 8.451 0.01 1 160 83 83 ARG N N 124.090 0.1 1 161 84 84 ALA H H 7.956 0.01 1 162 84 84 ALA N N 120.664 0.1 1 163 85 85 ASN H H 7.982 0.01 1 164 85 85 ASN N N 116.586 0.1 1 165 86 86 THR H H 7.727 0.01 1 166 86 86 THR N N 110.204 0.1 1 167 87 87 VAL H H 7.880 0.01 1 168 87 87 VAL N N 124.080 0.1 1 169 88 88 TYR H H 8.379 0.01 1 170 88 88 TYR N N 125.533 0.1 1 171 89 89 GLY H H 8.612 0.01 1 172 89 89 GLY N N 108.328 0.1 1 173 90 90 LEU H H 8.285 0.01 1 174 90 90 LEU N N 121.912 0.1 1 175 91 91 GLY H H 9.251 0.01 1 176 91 91 GLY N N 106.558 0.1 1 177 92 92 PHE H H 9.313 0.01 1 178 92 92 PHE N N 124.349 0.1 1 179 93 93 ALA H H 9.808 0.01 1 180 93 93 ALA N N 121.497 0.1 1 181 94 94 SER H H 7.568 0.01 1 182 94 94 SER N N 107.464 0.1 1 183 95 95 GLU H H 9.622 0.01 1 184 95 95 GLU N N 124.842 0.1 1 185 96 96 GLN H H 8.461 0.01 1 186 96 96 GLN N N 119.791 0.1 1 187 97 97 HIS H H 7.796 0.01 1 188 97 97 HIS N N 118.476 0.1 1 189 98 98 LEU H H 7.174 0.01 1 190 98 98 LEU N N 122.561 0.1 1 191 99 99 THR H H 8.557 0.01 1 192 99 99 THR N N 117.203 0.1 1 193 100 100 GLN H H 8.222 0.01 1 194 100 100 GLN N N 119.775 0.1 1 195 101 101 PHE H H 7.876 0.01 1 196 101 101 PHE N N 120.855 0.1 1 197 102 102 ALA H H 8.727 0.01 1 198 102 102 ALA N N 122.462 0.1 1 199 103 103 GLU H H 8.456 0.01 1 200 103 103 GLU N N 119.444 0.1 1 201 104 104 LYS H H 7.263 0.01 1 202 104 104 LYS N N 121.075 0.1 1 203 105 105 PHE H H 7.888 0.01 1 204 105 105 PHE N N 122.148 0.1 1 205 106 106 GLN H H 7.591 0.01 1 206 106 106 GLN N N 115.982 0.1 1 207 107 107 GLU H H 7.658 0.01 1 208 107 107 GLU N N 119.976 0.1 1 209 108 108 VAL H H 8.254 0.01 1 210 108 108 VAL N N 118.099 0.1 1 211 109 109 LYS H H 8.200 0.01 1 212 109 109 LYS N N 122.942 0.1 1 213 110 110 GLU H H 7.653 0.01 1 214 110 110 GLU N N 118.740 0.1 1 215 111 111 ALA H H 7.803 0.01 1 216 111 111 ALA N N 123.275 0.1 1 217 112 112 ALA H H 8.452 0.01 1 218 112 112 ALA N N 121.705 0.1 1 219 113 113 ARG H H 8.095 0.01 1 220 113 113 ARG N N 120.781 0.1 1 221 114 114 LEU H H 8.106 0.01 1 222 114 114 LEU N N 119.926 0.1 1 223 115 115 ALA H H 7.956 0.01 1 224 115 115 ALA N N 121.676 0.1 1 225 116 116 ARG H H 8.387 0.01 1 226 116 116 ARG N N 122.406 0.1 1 227 117 117 GLU H H 8.025 0.01 1 228 117 117 GLU N N 127.168 0.1 1 stop_ save_ save_heteronuclear_T1_list_1 _Saveframe_category T1_relaxation _Details 'relaxation times used (in ms): 12, 52, 102, 152, 202, 302, 402, 602, 902, 2002, 5002' loop_ _Software_label $CcpNmr_Analysis stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 600 _T1_coherence_type Nz _T1_value_units ms _Mol_system_component_name 'HOMER3A EVH1 domain' _Text_data_format . _Text_data . loop_ _T1_ID _Residue_seq_code _Residue_label _Atom_name _T1_value _T1_value_error 1 2 MET N 586.31 70.48 2 3 ALA N 662.42 317.37 3 4 ARG N 577.48 100.54 4 5 GLU N 613.13 40.79 5 6 GLN N 697.30 15.55 6 9 PHE N 640.36 93.13 7 10 SER N 632.33 73.64 8 13 ALA N 703.41 49.52 9 14 HIS N 735.01 53.12 10 15 VAL N 764.22 31.68 11 16 PHE N 739.70 46.54 12 18 ILE N 818.66 131.31 13 19 ASP N 735.15 79.21 14 22 THR N 714.17 16.51 15 23 LYS N 643.85 59.84 16 24 ARG N 688.06 36.14 17 26 TRP N 797.06 55.98 18 27 ILE N 835.16 57.86 19 29 ALA N 733.82 71.40 20 30 GLY N 1008.73 55.21 21 32 HIS N 633.28 99.70 22 33 ALA N 612.54 101.92 23 34 LEU N 700.83 48.71 24 35 THR N 730.97 67.72 25 36 VAL N 704.54 75.39 26 37 SER N 727.85 48.65 27 38 TYR N 732.83 127.98 28 39 PHE N 713.04 84.24 29 40 TYR N 723.15 42.77 30 41 ASP N 708.09 70.82 31 42 ALA N 740.31 53.84 32 43 THR N 736.93 63.04 33 44 ARG N 758.59 72.73 34 45 ASN N 701.05 25.86 35 46 VAL N 689.86 63.95 36 47 TYR N 694.42 87.22 37 48 ARG N 718.67 88.15 38 49 ILE N 738.30 68.37 39 50 ILE N 723.17 46.05 40 51 SER N 649.85 45.83 41 52 ILE N 708.42 42.88 42 53 GLY N 669.36 105.13 43 56 LYS N 652.78 73.65 44 57 ALA N 589.59 231.01 45 59 ILE N 720.15 66.98 46 60 ASN N 654.98 47.02 47 61 SER N 723.27 42.26 48 62 THR N 726.44 57.62 49 63 VAL N 674.59 48.28 50 64 THR N 847.27 57.58 51 67 MET N 760.26 139.14 52 68 THR N 677.71 62.67 53 69 PHE N 746.74 78.50 54 70 THR N 700.19 56.99 55 71 LYS N 690.27 35.16 56 72 THR N 791.95 56.42 57 73 SER N 736.54 69.88 58 74 GLN N 641.83 25.37 59 76 PHE N 652.50 59.43 60 77 GLY N 699.90 40.26 61 78 GLN N 673.27 49.26 62 79 TRP N 692.06 34.33 63 80 ALA N 727.32 46.20 64 81 ASP N 741.33 195.71 65 83 ARG N 627.49 63.20 66 84 ALA N 728.49 27.28 67 85 ASN N 747.57 59.88 68 86 THR N 763.00 58.38 69 87 VAL N 732.95 70.14 70 89 GLY N 668.84 51.72 71 90 LEU N 671.26 41.51 72 91 GLY N 729.44 101.41 73 92 PHE N 738.74 56.44 74 93 ALA N 599.06 52.27 75 94 SER N 757.64 41.69 76 95 GLU N 615.08 119.91 77 98 LEU N 762.27 99.05 78 100 GLN N 723.23 72.10 79 101 PHE N 788.68 50.41 80 102 ALA N 714.57 54.00 81 104 LYS N 744.10 53.19 82 105 PHE N 721.00 42.29 83 107 GLU N 761.85 41.97 84 108 VAL N 706.96 49.23 85 110 GLU N 725.94 33.02 86 111 ALA N 765.03 73.99 87 112 ALA N 719.25 24.01 88 113 ARG N 705.90 34.77 89 115 ALA N 742.19 33.59 90 116 ARG N 636.83 100.07 91 117 GLU N 998.31 34.41 stop_ save_ save_heteronuclear_T2_list_1 _Saveframe_category T2_relaxation _Details 'relaxation times used (in ms): 6, 10, 18, 26, 34, 42, 82, 122, 162, 202, 242' loop_ _Software_label $CcpNmr_Analysis stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 600 _T2_coherence_type Nx _T2_value_units ms _Mol_system_component_name 'HOMER3A EVH1 domain' _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 2 MET N 200.55 14.49 . . 2 3 ALA N 193.19 27.47 . . 3 4 ARG N 187.65 22.57 . . 4 5 GLU N 144.00 7.30 . . 5 6 GLN N 124.12 5.56 . . 6 9 PHE N 82.69 3.39 . . 7 10 SER N 74.40 5.66 . . 8 13 ALA N 81.90 2.27 . . 9 14 HIS N 80.35 3.29 . . 10 15 VAL N 91.39 4.34 . . 11 16 PHE N 81.40 3.08 . . 12 18 ILE N 69.84 2.46 . . 13 19 ASP N 82.51 2.58 . . 14 22 THR N 78.92 7.99 . . 15 23 LYS N 83.36 3.42 . . 16 24 ARG N 85.51 4.13 . . 17 26 TRP N 85.56 5.22 . . 18 27 ILE N 73.70 3.85 . . 19 29 ALA N 87.56 4.23 . . 20 30 GLY N 164.49 7.15 . . 21 32 HIS N 89.57 7.59 . . 22 33 ALA N 75.38 6.49 . . 23 34 LEU N 82.68 5.57 . . 24 35 THR N 76.56 2.65 . . 25 36 VAL N 69.29 4.50 . . 26 37 SER N 81.58 4.64 . . 27 38 TYR N 78.15 3.90 . . 28 39 PHE N 75.60 3.59 . . 29 40 TYR N 81.77 5.00 . . 30 41 ASP N 82.41 3.94 . . 31 42 ALA N 87.09 2.97 . . 32 43 THR N 85.45 2.29 . . 33 44 ARG N 88.59 3.75 . . 34 45 ASN N 83.21 3.80 . . 35 46 VAL N 84.50 9.30 . . 36 47 TYR N 84.66 5.45 . . 37 48 ARG N 80.09 3.62 . . 38 49 ILE N 82.94 5.88 . . 39 50 ILE N 81.09 1.94 . . 40 51 SER N 71.88 4.02 . . 41 52 ILE N 66.14 3.13 . . 42 53 GLY N 80.67 8.35 . . 43 56 LYS N 106.22 3.10 . . 44 57 ALA N 106.07 10.27 . . 45 59 ILE N 74.16 3.57 . . 46 60 ASN N 81.87 3.04 . . 47 61 SER N 86.65 3.50 . . 48 62 THR N 85.00 3.44 . . 49 63 VAL N 83.46 3.19 . . 50 64 THR N 76.68 6.26 . . 51 67 MET N 77.48 2.74 . . 52 68 THR N 80.94 6.75 . . 53 69 PHE N 73.93 2.76 . . 54 70 THR N 67.33 4.40 . . 55 71 LYS N 92.04 4.43 . . 56 72 THR N 51.26 5.36 . . 57 73 SER N 76.04 3.78 . . 58 74 GLN N 119.75 4.19 . . 59 76 PHE N 70.80 3.94 . . 60 77 GLY N 80.41 3.18 . . 61 78 GLN N 82.14 3.54 . . 62 79 TRP N 67.90 6.78 . . 63 80 ALA N 73.28 1.50 . . 64 81 ASP N 80.56 3.22 . . 65 83 ARG N 82.63 8.62 . . 66 84 ALA N 90.97 1.35 . . 67 85 ASN N 86.77 4.72 . . 68 86 THR N 53.60 6.22 . . 69 87 VAL N 82.43 4.41 . . 70 89 GLY N 70.93 6.02 . . 71 90 LEU N 83.15 3.37 . . 72 91 GLY N 73.92 5.67 . . 73 92 PHE N 82.71 4.20 . . 74 93 ALA N 76.16 4.50 . . 75 94 SER N 72.36 6.91 . . 76 95 GLU N 56.17 5.00 . . 77 98 LEU N 75.38 2.78 . . 78 100 GLN N 71.89 1.22 . . 79 101 PHE N 66.54 4.83 . . 80 102 ALA N 70.19 1.57 . . 81 104 LYS N 72.17 2.21 . . 82 105 PHE N 65.04 3.29 . . 83 107 GLU N 73.66 1.35 . . 84 108 VAL N 65.82 3.11 . . 85 110 GLU N 74.53 1.97 . . 86 111 ALA N 73.09 2.79 . . 87 112 ALA N 75.24 3.07 . . 88 113 ARG N 89.32 6.94 . . 89 115 ALA N 89.29 2.09 . . 90 116 ARG N 228.87 23.79 . . 91 117 GLU N 534.63 69.36 . . stop_ save_