data_15441 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone chemical shift assignments of dynein intermediate chain IC74 residues 84-143 ; _BMRB_accession_number 15441 _BMRB_flat_file_name bmr15441.str _Entry_type original _Submission_date 2007-08-23 _Accession_date 2007-08-23 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Benison Gregory C. . 2 Barbar Elisar J. . 3 Berkholz Donald S. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 heteronucl_NOE 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 55 "13C chemical shifts" 111 "15N chemical shifts" 55 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2008-07-03 original author . stop_ _Original_release_date 2008-07-03 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Protein Assignments without Peak Lists Using Higher-Order Spectra' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 17919953 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Benison Gregory C. . 2 Berkholz Donald S. . 3 Barbar Elisar J. . stop_ _Journal_abbreviation 'J. Magn. Reson.' _Journal_volume 189 _Journal_issue 2 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 173 _Page_last 181 _Year 2007 _Details . loop_ _Keyword burrow-owl dynein 'higher-order spectrum' 'intermediate chain' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name IC74 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label IC74 $IC74 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_IC74 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common IC74 _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 75 _Mol_residue_sequence ; SHHHHHHSMANSAGSSNTQT PDASLQATVNGQSGGKKQPL NLSVYNVQATNIPPKETLVY TKQTQTTSTGGGNGD ; loop_ _Residue_seq_code _Residue_label 1 SER 2 HIS 3 HIS 4 HIS 5 HIS 6 HIS 7 HIS 8 SER 9 MET 10 ALA 11 ASN 12 SER 13 ALA 14 GLY 15 SER 16 SER 17 ASN 18 THR 19 GLN 20 THR 21 PRO 22 ASP 23 ALA 24 SER 25 LEU 26 GLN 27 ALA 28 THR 29 VAL 30 ASN 31 GLY 32 GLN 33 SER 34 GLY 35 GLY 36 LYS 37 LYS 38 GLN 39 PRO 40 LEU 41 ASN 42 LEU 43 SER 44 VAL 45 TYR 46 ASN 47 VAL 48 GLN 49 ALA 50 THR 51 ASN 52 ILE 53 PRO 54 PRO 55 LYS 56 GLU 57 THR 58 LEU 59 VAL 60 TYR 61 THR 62 LYS 63 GLN 64 THR 65 GLN 66 THR 67 THR 68 SER 69 THR 70 GLY 71 GLY 72 GLY 73 ASN 74 GLY 75 ASP stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-31 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value GB AAC70933 "cytoplasmic dynein intermediate chain isoform DIC1a [Drosophila melanogaster]" 80.00 634 100.00 100.00 1.18e-30 GB AAC70934 "cytoplasmic dynein intermediate chain isoform DIC1b [Drosophila melanogaster]" 80.00 627 100.00 100.00 1.20e-30 GB AAC70935 "cytoplasmic dynein intermediate chain isoform DIC1c [Drosophila melanogaster]" 80.00 626 100.00 100.00 1.11e-30 GB AAC70938 "cytoplasmic dynein intermediate chain isoform DIC2c [Drosophila melanogaster]" 80.00 650 100.00 100.00 1.21e-30 GB AAC70939 "cytoplasmic dynein intermediate chain isoform DIC3a [Drosophila melanogaster]" 80.00 636 100.00 100.00 1.25e-30 REF NP_001285486 "short wing, isoform N [Drosophila melanogaster]" 80.00 634 100.00 100.00 1.21e-30 REF NP_001285487 "short wing, isoform O [Drosophila melanogaster]" 80.00 643 100.00 100.00 1.12e-30 REF NP_477070 "short wing, isoform G [Drosophila melanogaster]" 80.00 636 100.00 100.00 1.20e-30 REF NP_477072 "short wing, isoform I [Drosophila melanogaster]" 80.00 627 100.00 100.00 1.00e-30 REF NP_477074 "short wing, isoform A [Drosophila melanogaster]" 80.00 634 100.00 100.00 1.21e-30 SP Q24246 "RecName: Full=Cytoplasmic dynein 1 intermediate chain; Short=DH IC; AltName: Full=Dynein intermediate chain, cytosolic; AltName" 80.00 663 100.00 100.00 1.35e-30 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $IC74 'fruit fly' 7227 Eukaryota Metazoa Drosophila melanogaster stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $IC74 'recombinant technology' 'E. coli' Escherichia coli . PET15d stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $IC74 1 mM '[U-100% 13C; U-100% 15N]' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' 'potassium phosphate' 50 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_xwinnmr _Saveframe_category software _Name xwinnmr _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ save_burrow-owl _Saveframe_category software _Name Burrow-Owl _Version . loop_ _Vendor _Address _Electronic_address 'Benison, Berkholz, and Barbar' ; Biochemistry & Biophysics Oregon State University Corvallis, OR 97331 ; www.burrow-owl.org stop_ loop_ _Task 'chemical shift assignment' 'data analysis' stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_1H-15N_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ save_2D_1H-15N_hetnoe_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N hetnoe' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.25 . M pH 6.5 . pH pressure 1 . atm temperature 293 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio _Indirect_shift_ratio_citation_label _Correction_value_citation_label DSS C 13 'methyl protons' ppm 0.00 . indirect . . . 0.251449530 $entry_citation $entry_citation DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 $entry_citation $entry_citation DSS N 15 'methyl protons' ppm 0.00 . indirect . . . 0.101329118 $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D CBCA(CO)NH' '3D HNCACB' '3D 1H-15N NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name IC74 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 84 16 SER H H 8.46 0.02 1 2 84 16 SER CA C 58.41 0.05 1 3 84 16 SER CB C 63.90 0.05 1 4 84 16 SER N N 117.04 0.05 1 5 85 17 ASN H H 8.51 0.02 1 6 85 17 ASN CA C 53.39 0.05 1 7 85 17 ASN CB C 38.80 0.05 1 8 85 17 ASN N N 120.86 0.05 1 9 86 18 THR H H 8.16 0.02 1 10 86 18 THR CA C 62.17 0.05 1 11 86 18 THR CB C 69.70 0.05 1 12 86 18 THR N N 114.28 0.05 1 13 87 19 GLN H H 8.50 0.02 1 14 87 19 GLN CA C 55.90 0.05 1 15 87 19 GLN CB C 29.24 0.05 1 16 87 19 GLN N N 123.01 0.05 1 17 88 20 THR H H 8.32 0.02 1 18 88 20 THR CA C 60.00 0.05 1 19 88 20 THR CB C 69.61 0.05 1 20 88 20 THR N N 118.22 0.05 1 21 89 21 PRO CA C 63.27 0.05 1 22 89 21 PRO CB C 32.06 0.05 1 23 90 22 ASP H H 8.42 0.02 1 24 90 22 ASP CA C 54.24 0.05 1 25 90 22 ASP CB C 41.06 0.05 1 26 90 22 ASP N N 120.68 0.05 1 27 91 23 ALA H H 8.43 0.02 1 28 91 23 ALA CA C 53.23 0.05 1 29 91 23 ALA CB C 19.04 0.05 1 30 91 23 ALA N N 125.89 0.05 1 31 92 24 SER H H 8.41 0.02 1 32 92 24 SER CA C 59.19 0.05 1 33 92 24 SER CB C 63.27 0.05 1 34 92 24 SER N N 114.73 0.05 1 35 93 25 LEU H H 8.00 0.02 1 36 93 25 LEU CA C 55.43 0.05 1 37 93 25 LEU CB C 42.25 0.05 1 38 93 25 LEU N N 123.36 0.05 1 39 94 26 GLN H H 8.17 0.02 1 40 94 26 GLN CA C 55.74 0.05 1 41 94 26 GLN CB C 29.24 0.05 1 42 94 26 GLN N N 120.26 0.05 1 43 95 27 ALA H H 8.34 0.02 1 44 95 27 ALA CA C 52.71 0.05 1 45 95 27 ALA CB C 19.22 0.05 1 46 95 27 ALA N N 125.62 0.05 1 47 96 28 THR H H 8.14 0.02 1 48 96 28 THR CA C 61.72 0.05 1 49 96 28 THR CB C 69.77 0.05 1 50 96 28 THR N N 112.84 0.05 1 51 97 29 VAL H H 8.21 0.02 1 52 97 29 VAL CA C 62.48 0.05 1 53 97 29 VAL CB C 32.73 0.05 1 54 97 29 VAL N N 122.24 0.05 1 55 98 30 ASN H H 8.56 0.02 1 56 98 30 ASN CA C 53.61 0.05 1 57 98 30 ASN CB C 38.86 0.05 1 58 98 30 ASN N N 121.99 0.05 1 59 99 31 GLY H H 8.39 0.02 1 60 99 31 GLY CA C 45.45 0.05 1 61 99 31 GLY N N 109.15 0.05 1 62 100 32 GLN H H 8.27 0.02 1 63 100 32 GLN CA C 55.83 0.05 1 64 100 32 GLN CB C 29.45 0.05 1 65 100 32 GLN N N 119.71 0.05 1 66 101 33 SER H H 8.62 0.02 1 67 101 33 SER CA C 58.63 0.05 1 68 101 33 SER CB C 63.81 0.05 1 69 101 33 SER N N 117.77 0.05 1 70 102 34 GLY H H 8.52 0.02 1 71 102 34 GLY CA C 45.61 0.05 1 72 102 34 GLY N N 111.06 0.05 1 73 103 35 GLY H H 8.32 0.02 1 74 103 35 GLY CA C 45.30 0.05 1 75 103 35 GLY N N 108.69 0.05 1 76 104 36 LYS H H 8.22 0.02 1 77 104 36 LYS CA C 56.28 0.05 1 78 104 36 LYS CB C 33.22 0.05 1 79 104 36 LYS N N 120.86 0.05 1 80 105 37 LYS H H 8.46 0.02 1 81 105 37 LYS CA C 55.96 0.05 1 82 105 37 LYS CB C 29.29 0.05 1 83 105 37 LYS N N 123.15 0.05 1 84 106 38 GLN H H 8.88 0.02 1 85 106 38 GLN CA C 55.81 0.05 1 86 106 38 GLN CB C 29.14 0.05 1 87 106 38 GLN N N 120.71 0.05 1 88 107 39 PRO CA C 63.03 0.05 1 89 107 39 PRO CB C 31.99 0.05 1 90 108 40 LEU H H 8.39 0.02 1 91 108 40 LEU CA C 55.23 0.05 1 92 108 40 LEU CB C 42.50 0.05 1 93 108 40 LEU N N 122.34 0.05 1 94 109 41 ASN H H 8.47 0.02 1 95 109 41 ASN CA C 52.92 0.05 1 96 109 41 ASN CB C 38.59 0.05 1 97 109 41 ASN N N 119.41 0.05 1 98 110 42 LEU H H 8.27 0.02 1 99 110 42 LEU CA C 55.37 0.05 1 100 110 42 LEU CB C 42.10 0.05 1 101 110 42 LEU N N 122.91 0.05 1 102 111 43 SER H H 8.34 0.02 1 103 111 43 SER CA C 58.63 0.05 1 104 111 43 SER CB C 63.57 0.05 1 105 111 43 SER N N 116.52 0.05 1 106 112 44 VAL H H 7.92 0.02 1 107 112 44 VAL CA C 62.44 0.05 1 108 112 44 VAL CB C 32.62 0.05 1 109 112 44 VAL N N 120.57 0.05 1 110 113 45 TYR H H 8.11 0.02 1 111 113 45 TYR CA C 58.01 0.05 1 112 113 45 TYR CB C 38.74 0.05 1 113 113 45 TYR N N 122.78 0.05 1 114 114 46 ASN H H 8.26 0.02 1 115 114 46 ASN CA C 53.07 0.05 1 116 114 46 ASN CB C 39.06 0.05 1 117 114 46 ASN N N 121.03 0.05 1 118 115 47 VAL H H 8.05 0.02 1 119 115 47 VAL CA C 62.47 0.05 1 120 115 47 VAL CB C 32.79 0.05 1 121 115 47 VAL N N 120.81 0.05 1 122 116 48 GLN H H 8.42 0.02 1 123 116 48 GLN CA C 55.94 0.05 1 124 116 48 GLN CB C 29.03 0.05 1 125 116 48 GLN N N 123.64 0.05 1 126 117 49 ALA H H 8.23 0.02 1 127 117 49 ALA CA C 52.76 0.05 1 128 117 49 ALA CB C 19.32 0.05 1 129 117 49 ALA N N 124.52 0.05 1 130 118 50 THR H H 8.13 0.02 1 131 118 50 THR CA C 62.15 0.05 1 132 118 50 THR CB C 69.63 0.05 1 133 118 50 THR N N 113.59 0.05 1 134 119 51 ASN H H 8.43 0.02 1 135 119 51 ASN CA C 53.23 0.05 1 136 119 51 ASN CB C 38.74 0.05 1 137 119 51 ASN N N 121.01 0.05 1 138 120 52 ILE H H 8.11 0.02 1 139 120 52 ILE CA C 58.33 0.05 1 140 120 52 ILE CB C 38.59 0.05 1 141 120 52 ILE N N 123.23 0.05 1 142 122 54 PRO CA C 62.49 0.05 1 143 122 54 PRO CB C 31.90 0.05 1 144 123 55 LYS H H 8.45 0.02 1 145 123 55 LYS CA C 56.53 0.05 1 146 123 55 LYS CB C 32.84 0.05 1 147 123 55 LYS N N 121.85 0.05 1 148 124 56 GLU H H 8.49 0.02 1 149 124 56 GLU CA C 56.37 0.05 1 150 124 56 GLU CB C 29.86 0.05 1 151 124 56 GLU N N 122.15 0.05 1 152 125 57 THR H H 8.26 0.02 1 153 125 57 THR CA C 62.17 0.05 1 154 125 57 THR CB C 69.70 0.05 1 155 125 57 THR N N 116.21 0.05 1 156 126 58 LEU H H 8.25 0.02 1 157 126 58 LEU CA C 55.12 0.05 1 158 126 58 LEU CB C 42.10 0.05 1 159 126 58 LEU N N 125.44 0.05 1 160 127 59 VAL H H 8.03 0.02 1 161 127 59 VAL CA C 62.17 0.05 1 162 127 59 VAL CB C 32.53 0.05 1 163 127 59 VAL N N 121.08 0.05 1 164 128 60 TYR H H 8.35 0.02 1 165 128 60 TYR CA C 57.94 0.05 1 166 128 60 TYR CB C 38.96 0.05 1 167 128 60 TYR N N 124.52 0.05 1 168 129 61 THR H H 8.08 0.02 1 169 129 61 THR CA C 61.61 0.05 1 170 129 61 THR CB C 69.93 0.05 1 171 129 61 THR N N 116.83 0.05 1 172 130 62 LYS H H 8.31 0.02 1 173 130 62 LYS CA C 56.75 0.05 1 174 130 62 LYS CB C 33.07 0.05 1 175 130 62 LYS N N 123.90 0.05 1 176 131 63 GLN H H 8.56 0.02 1 177 131 63 GLN CA C 56.75 0.05 1 178 131 63 GLN CB C 30.23 0.05 1 179 131 63 GLN N N 121.91 0.05 1 180 132 64 THR H H 8.26 0.02 1 181 132 64 THR CA C 61.93 0.05 1 182 132 64 THR CB C 69.77 0.05 1 183 132 64 THR N N 115.96 0.05 1 184 133 65 GLN H H 8.43 0.02 1 185 133 65 GLN CA C 56.12 0.05 1 186 133 65 GLN CB C 29.61 0.05 1 187 133 65 GLN N N 122.78 0.05 1 188 134 66 THR H H 8.37 0.02 1 189 134 66 THR CA C 61.93 0.05 1 190 134 66 THR CB C 69.77 0.05 1 191 134 66 THR N N 115.98 0.05 1 192 135 67 THR CA C 61.54 0.05 1 193 135 67 THR CB C 69.78 0.05 1 194 136 68 SER H H 8.49 0.02 1 195 136 68 SER CA C 58.48 0.05 1 196 136 68 SER CB C 63.62 0.05 1 197 136 68 SER N N 118.29 0.05 1 198 137 69 THR H H 8.32 0.02 1 199 137 69 THR CA C 61.86 0.05 1 200 137 69 THR CB C 69.86 0.05 1 201 137 69 THR N N 115.56 0.05 1 202 138 70 GLY H H 8.47 0.02 1 203 138 70 GLY CA C 45.45 0.05 1 204 138 70 GLY N N 111.26 0.05 1 205 139 71 GLY H H 8.39 0.02 1 206 139 71 GLY CA C 44.92 0.05 1 207 139 71 GLY N N 109.03 0.05 1 208 140 72 GLY H H 8.42 0.02 1 209 140 72 GLY CA C 45.08 0.05 1 210 140 72 GLY N N 109.05 0.05 1 211 141 73 ASN H H 8.50 0.02 1 212 141 73 ASN CA C 53.14 0.05 1 213 141 73 ASN CB C 39.18 0.05 1 214 141 73 ASN N N 118.52 0.05 1 215 142 74 GLY H H 8.47 0.02 1 216 142 74 GLY CA C 45.14 0.05 1 217 142 74 GLY N N 110.02 0.05 1 218 143 75 ASP H H 7.96 0.02 1 219 143 75 ASP CA C 55.81 0.05 1 220 143 75 ASP CB C 42.00 0.05 1 221 143 75 ASP N N 125.84 0.05 1 stop_ save_ save_heteronuclear_noe_list_1 _Saveframe_category heteronuclear_NOE _Details . loop_ _Experiment_label '2D 1H-15N hetnoe' stop_ loop_ _Sample_label . stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 600 _Mol_system_component_name IC74 _Atom_one_atom_name H _Atom_two_atom_name N _Heteronuclear_NOE_value_type 'relative intensities' _NOE_reference_value 1 _NOE_reference_description . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Heteronuclear_NOE_value _Heteronuclear_NOE_value_error 16 SER -7.07 -0.67 17 ASN -7.02 -0.60 18 THR -4.92 -0.39 19 GLN -6.79 -0.35 20 THR -4.36 -0.33 22 ASP -5.49 -0.39 23 ALA -9.02 -0.41 24 SER -1.43 -0.11 25 LEU -0.68 -0.04 26 GLN -1.55 -0.11 27 ALA -2.34 -0.18 28 THR -3.01 -0.25 29 VAL -0.15 -0.01 30 ASN 0.01 0.00 31 GLY -29.19 -1.25 32 GLN -0.23 -0.02 33 SER -0.26 -0.04 34 GLY -6.73 -0.48 35 GLY -5.89 -0.52 36 LYS -1.65 -0.11 37 LYS -8.63 -0.27 38 GLN 0.07 0.01 40 LEU -3.29 -0.25 41 ASN -1.80 -0.14 42 LEU 0.05 0.00 43 SER -3.96 -0.20 44 VAL 1.86 0.13 45 TYR -2.41 -0.19 46 ASN -0.95 -0.06 47 VAL -3.42 -0.23 48 GLN -2.81 -0.21 49 ALA 0.15 0.01 50 THR -2.70 -0.19 51 ASN -2.68 -0.20 52 ILE -3.52 -0.22 55 LYS -1.92 -0.12 56 GLU -1.45 -0.07 57 THR -4.60 -0.13 58 LEU 0.51 0.04 59 VAL -3.12 -0.13 60 TYR -1.74 -0.12 61 THR -2.21 -0.17 62 LYS -0.00 -0.00 63 GLN 0.72 0.03 64 THR -1.92 -0.09 65 GLN -6.96 -0.50 66 THR -4.84 -0.48 68 SER -7.59 -0.69 69 THR -9.13 -1.01 70 GLY -15.26 -1.12 71 GLY -30.59 -1.59 72 GLY -40.07 -1.73 73 ASN -27.46 -1.57 74 GLY -26.98 -2.79 75 ASP -27.86 -1.79 stop_ save_