data_15113 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution Structure of the Rous Sarcoma Virus Nucleocapsid Protein:muPsi RNA Packaging Signal Complex ; _BMRB_accession_number 15113 _BMRB_flat_file_name bmr15113.str _Entry_type new _Submission_date 2007-01-24 _Accession_date 2007-01-24 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Zhou J. . . 2 Summers M. F. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 2 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 551 "13C chemical shifts" 88 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2007-03-06 update BMRB 'chemical shift reference update' 2007-03-05 original author 'Original release' stop_ save_ ############################# # Citation for this entry # ############################# save_citations _Saveframe_category entry_citation _Citation_full . _Citation_title ; Solution Structure of the Rous Sarcoma Virus Nucleocaspid Protein:muPsi RNA Packaging Signal Complex ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 17070546 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Zhou J. . . 2 Bean R. L. . 3 Vogt V. M. . 4 Summers M. F. . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_volume 365 _Journal_issue 2 _Journal_CSD 0353 _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 453 _Page_last 467 _Year 2007 _Details . loop_ _Keyword 'Rous sarcoma virus' 'ribonucleic acid (RNA)' psi-site 'nucleocapsid (NC) protein' 'UNCG tetraloop' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'NC:muPsi complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label Zn $ZN2 'muPsi RNA' $muPsi_RNA 'NC protein' $Nucleocapsid_(NC)_Protein stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_muPsi_RNA _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common muPsi_RNA _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 75 _Mol_residue_sequence ; CUGCCCUCAUCCGUCUCGCU UAUUCGGGGAGCGGACGAUG ACCCUAGUAGAGGGGGCUGC GGCUUAGGAGGGCAG ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 160 C 2 161 U 3 162 G 4 163 C 5 164 C 6 165 C 7 166 U 8 167 C 9 168 A 10 169 U 11 170 C 12 171 C 13 172 G 14 173 U 15 174 C 16 175 U 17 176 C 18 177 G 19 178 C 20 179 U 21 180 U 22 181 A 23 182 U 24 183 U 25 184 C 26 185 G 27 186 G 28 187 G 29 188 G 30 189 A 31 190 G 32 191 C 33 192 G 34 193 G 35 194 A 36 195 C 37 196 G 38 197 A 39 198 U 40 199 G 41 200 A 42 201 C 43 202 C 44 203 C 45 204 U 46 205 A 47 206 G 48 207 U 49 208 A 50 209 G 51 210 A 52 211 G 53 212 G 54 213 G 55 214 G 56 215 G 57 216 C 58 217 U 59 218 G 60 219 C 61 220 G 62 221 G 63 222 C 64 223 U 65 224 U 66 225 A 67 226 G 68 227 G 69 228 A 70 229 G 71 230 G 72 231 G 73 232 C 74 233 A 75 234 G stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ save_Nucleocapsid_(NC)_Protein _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Nucleocapsid_(NC)_Protein _Molecular_mass . _Mol_thiol_state 'all other bound' _Details . _Residue_count 61 _Mol_residue_sequence ; GRARGLCYTCGSPGHYQAQC PKKRKSGNSRERCQLCNGMG HNAKQCRKRDGNQGQRPGKG L ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 15 GLY 2 16 ARG 3 17 ALA 4 18 ARG 5 19 GLY 6 20 LEU 7 21 CYS 8 22 TYR 9 23 THR 10 24 CYS 11 25 GLY 12 26 SER 13 27 PRO 14 28 GLY 15 29 HIS 16 30 TYR 17 31 GLN 18 32 ALA 19 33 GLN 20 34 CYS 21 35 PRO 22 36 LYS 23 37 LYS 24 38 ARG 25 39 LYS 26 40 SER 27 41 GLY 28 42 ASN 29 43 SER 30 44 ARG 31 45 GLU 32 46 ARG 33 47 CYS 34 48 GLN 35 49 LEU 36 50 CYS 37 51 ASN 38 52 GLY 39 53 MET 40 54 GLY 41 55 HIS 42 56 ASN 43 57 ALA 44 58 LYS 45 59 GLN 46 60 CYS 47 61 ARG 48 62 LYS 49 63 ARG 50 64 ASP 51 65 GLY 52 66 ASN 53 67 GLN 54 68 GLY 55 69 GLN 56 70 ARG 57 71 PRO 58 72 GLY 59 73 LYS 60 74 GLY 61 75 LEU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ ############# # Ligands # ############# save_ZN _Saveframe_category ligand _Mol_type non-polymer _Name_common "ZN (ZINC ION)" _BMRB_code . _PDB_code ZN _Molecular_mass 65.409 _Mol_charge 2 _Mol_paramagnetic . _Mol_aromatic . _Details ; Information obtained from PDB's Chemical Component Dictionary at http://wwpdb-remediation.rutgers.edu/downloads.html Downloaded on Wed Dec 20 05:48:03 2006 ; loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons ZN ZN ZN N 2 . ? stop_ _Mol_thiol_state . _Sequence_homology_query_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $muPsi_RNA . 11886 Viruses . Alpharetrovirus 'Rous sarcoma virus' $Nucleocapsid_(NC)_Protein . 11886 Viruses . Alpharetrovirus 'Rous sarcoma virus' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_type _Vector_name $Nucleocapsid_(NC)_Protein 'recombinant technology' bacteria . . 'BL21 codon plus (DE3)-RIL' Plasmid pGEX-6P-1 $muPsi_RNA 'enzymatic semi-synthesis' . . . . plasmid . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details '1.0 mM unlabeled nucleocapsid protein, 1.0 mM A-selectively-protonated muPsi RNA, 5 mM Tris-d11-HCl buffer, pH 7.0, 5mM NaCl, 0.1 mM ZnCl2, 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $muPsi_RNA 1.0 mM 'G, C, U 2H-enriched' $Nucleocapsid_(NC)_Protein 1.0 mM 'natural abundance' Tris-d11-HCl 5 mM . NaCL 5 mM . ZnCl2 0.1 mM . D2O 100 % . stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details '1.2 mM unlabeled nucleocapsid protein, 1.2 mM G-selectively-protonated muPsi RNA, 5 mM Tris-d11-HCl buffer, pH 7.0, 5mM NaCl, 0.1 mM ZnCl2, 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $muPsi_RNA 1.2 mM 'A, C, U 2H-enriched' $Nucleocapsid_(NC)_Protein 1.2 mM 'natural abundance' Tris-d11-HCl 5 mM . NaCL 5 mM . ZnCl2 0.1 mM . D2O 100 % . stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details '0.8 mM unlabeled nucleocapsid protein, 0.8 mM U-selectively-protonated muPsi RNA, 5 mM Tris-d11-HCl buffer, pH 7.0, 5mM NaCl, 0.1 mM ZnCl2, 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $muPsi_RNA 0.8 mM 'A, G, C 2H-enriched' $Nucleocapsid_(NC)_Protein 0.8 mM 'natural abundance' Tris-d11-HCl 5 mM . NaCL 5 mM . ZnCl2 0.1 mM . D2O 100 % . stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details '0.8 mM unlabeled nucleocapsid protein, 0.8 mM C-selectively-protonated muPsi RNA, 5 mM Tris-d11-HCl buffer, pH 7.0, 5mM NaCl, 0.1 mM ZnCl2, 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $muPsi_RNA 0.8 mM 'A, G, U 2H-enriched' $Nucleocapsid_(NC)_Protein 0.8 mM 'natural abundance' Tris-d11-HCl 5 mM . NaCL 5 mM . ZnCl2 0.1 mM . D2O 100 % . stop_ save_ save_sample_5 _Saveframe_category sample _Sample_type solution _Details '1.2 mM unlabeled nucleocapsid protein, 1.2 mM unlabeled muPsi RNA, 5 mM Tris-d11-HCl buffer, pH 7.0, 5mM NaCl, 0.1 mM ZnCl2, 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $muPsi_RNA 1.2 mM 'natural abundance' $Nucleocapsid_(NC)_Protein 1.2 mM 'natural abundance' Tris-d11-HCl 5 mM . NaCL 5 mM . ZnCl2 0.1 mM . D2O 100 % . stop_ save_ save_sample_6 _Saveframe_category sample _Sample_type solution _Details '1.2 mM 15N, 13C-labeled nucleocapsid protein, 1.2 mM unlabeled muPsi RNA, 5 mM Tris-d11-HCl buffer, pH 7.0; 5mM NaCl, 0.1 mM ZnCl2, 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling 'muPsi RNA' 1.2 mM 'natural abundance' 'Nucleocapsid (NC) Protein' 1.2 mM '[U-98% 13C; U-98% 15N]' Tris-d11-HCl 5 mM . NaCL 5 mM . ZnCl2 0.1 mM . D2O 100 % . stop_ save_ ############################ # Computer software used # ############################ save_XWINNMR _Saveframe_category software _Name XWINNMR _Version . loop_ _Vendor _Address _Electronic_address BRUKER . . stop_ loop_ _Task collection stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, F., Grzesiek. S., Vuister, G.W., Zhu, G., Pfeifer, J., & Bax, A.' . . stop_ loop_ _Task processing stop_ _Details . save_ save_NMRView _Saveframe_category software _Name NMRView _Version 5 loop_ _Vendor _Address _Electronic_address 'Johnson, B.A., Blevins, R.A.' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_CYANA _Saveframe_category software _Name CYANA _Version 2.1 loop_ _Vendor _Address _Electronic_address 'Guntert, P., Mumenthaler, C. & Wuthrich, K.' . . stop_ loop_ _Task refinement stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 800 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DMX _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _Sample_label $sample_1 save_ save_2D_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _Sample_label $sample_2 save_ save_2D_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _Sample_label $sample_3 save_ save_2D_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _Sample_label $sample_4 save_ save_2D_NOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _Sample_label $sample_5 save_ save_3D_13C-separated_NOESY_6 _Saveframe_category NMR_applied_experiment _Experiment_name 3D_13C-separated_NOESY _Sample_label $sample_6 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.0053 . n/a pH 7.0 . pH pressure 1 . atm temperature 308 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS C 13 'methyl protons' ppm 0 external indirect . . . 0.251449530 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1_2 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D NOESY' 3D_13C-separated_NOESY stop_ loop_ _Sample_label $sample_1 $sample_2 $sample_3 $sample_4 $sample_5 $sample_6 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'muPsi RNA' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 160 1 C H1' H 5.276 . 1 2 160 1 C H5 H 6.073 . 1 3 160 1 C H6 H 8.052 . 1 4 161 2 U H1' H 5.670 . 1 5 161 2 U H5 H 5.594 . 1 6 161 2 U H6 H 8.042 . 1 7 162 3 G H1' H 5.898 . 1 8 162 3 G H8 H 7.877 . 1 9 163 4 C H1' H 5.567 . 1 10 163 4 C H5 H 5.327 . 1 11 163 4 C H6 H 7.800 . 1 12 164 5 C H1' H 5.582 . 1 13 164 5 C H3' H 4.437 . 1 14 164 5 C H5 H 5.595 . 1 15 164 5 C H6 H 7.826 . 1 16 165 6 C H1' H 5.584 . 1 17 165 6 C H5 H 5.604 . 1 18 165 6 C H6 H 7.889 . 1 19 166 7 U H1' H 5.661 . 1 20 166 7 U H3' H 4.659 . 1 21 166 7 U H5 H 5.506 . 1 22 166 7 U H6 H 8.029 . 1 23 167 8 C H1' H 6.010 . 1 24 167 8 C H2' H 4.326 . 1 25 167 8 C H3' H 4.817 . 1 26 167 8 C H4' H 4.529 . 1 27 167 8 C H5 H 5.792 . 1 28 167 8 C H6 H 7.940 . 1 29 168 9 A H1' H 6.125 . 1 30 168 9 A H2 H 8.044 . 1 31 168 9 A H2' H 4.835 . 1 32 168 9 A H3' H 5.096 . 1 33 168 9 A H4' H 4.719 . 1 34 168 9 A H5' H 4.405 . 2 35 168 9 A H5'' H 4.306 . 2 36 168 9 A H8 H 8.761 . 1 37 169 10 U H1' H 6.113 . 1 38 169 10 U H2' H 4.585 . 1 39 169 10 U H3' H 4.866 . 1 40 169 10 U H4' H 4.679 . 1 41 169 10 U H5 H 6.073 . 1 42 169 10 U H5' H 4.519 . 2 43 169 10 U H5'' H 4.402 . 2 44 169 10 U H6 H 8.059 . 1 45 170 11 C H1' H 5.707 . 1 46 170 11 C H2' H 4.578 . 1 47 170 11 C H3' H 4.419 . 1 48 170 11 C H4' H 4.504 . 1 49 170 11 C H5 H 6.077 . 1 50 170 11 C H6 H 8.176 . 1 51 171 12 C H1' H 5.593 . 1 52 171 12 C H2' H 4.579 . 1 53 171 12 C H3' H 4.693 . 1 54 171 12 C H5 H 5.784 . 1 55 171 12 C H6 H 8.012 . 1 56 172 13 G H1' H 5.716 . 1 57 172 13 G H2' H 4.521 . 1 58 172 13 G H3' H 4.606 . 1 59 172 13 G H4' H 4.167 . 1 60 172 13 G H8 H 7.528 . 1 61 173 14 U H1' H 5.641 . 1 62 173 14 U H2' H 4.362 . 1 63 173 14 U H3' H 4.578 . 1 64 173 14 U H5 H 5.169 . 1 65 173 14 U H6 H 7.831 . 1 66 174 15 C H1' H 5.997 . 1 67 174 15 C H2' H 4.433 . 1 68 174 15 C H3' H 4.775 . 1 69 174 15 C H4' H 4.502 . 1 70 174 15 C H5 H 5.463 . 1 71 174 15 C H6 H 7.879 . 1 72 175 16 U H1' H 6.083 . 1 73 175 16 U H2' H 4.522 . 1 74 175 16 U H3' H 4.805 . 1 75 175 16 U H4' H 4.574 . 1 76 175 16 U H5 H 5.994 . 1 77 175 16 U H5' H 4.257 . 2 78 175 16 U H5'' H 4.362 . 2 79 175 16 U H6 H 7.973 . 1 80 176 17 C H1' H 5.680 . 1 81 176 17 C H2' H 4.671 . 1 82 176 17 C H3' H 4.302 . 1 83 176 17 C H4' H 4.567 . 1 84 176 17 C H5 H 5.736 . 1 85 176 17 C H5' H 4.437 . 2 86 176 17 C H6 H 7.762 . 1 87 177 18 G H1' H 5.867 . 1 88 177 18 G H2' H 4.733 . 1 89 177 18 G H3' H 4.682 . 1 90 177 18 G H4' H 4.603 . 1 91 177 18 G H8 H 7.868 . 1 92 178 19 C H1' H 5.651 . 1 93 178 19 C H2' H 4.525 . 1 94 178 19 C H3' H 4.663 . 1 95 178 19 C H4' H 4.188 . 1 96 178 19 C H5 H 5.319 . 1 97 178 19 C H6 H 7.778 . 1 98 179 20 U H1' H 5.678 . 1 99 179 20 U H2' H 4.673 . 1 100 179 20 U H3' H 4.518 . 1 101 179 20 U H4' H 4.193 . 1 102 179 20 U H5 H 5.487 . 1 103 179 20 U H6 H 7.885 . 1 104 180 21 U H1' H 5.644 . 1 105 180 21 U H2' H 4.657 . 1 106 180 21 U H3' H 4.522 . 1 107 180 21 U H4' H 4.363 . 1 108 180 21 U H5 H 5.869 . 1 109 180 21 U H6 H 7.948 . 1 110 181 22 A H1' H 5.938 . 1 111 181 22 A H2 H 7.664 . 1 112 181 22 A H2' H 4.220 . 1 113 181 22 A H3' H 4.632 . 1 114 181 22 A H4' H 4.554 . 1 115 181 22 A H5' H 4.352 . 2 116 181 22 A H5'' H 4.498 . 2 117 181 22 A H8 H 8.219 . 1 118 182 23 U H1' H 5.716 . 1 119 182 23 U H2' H 4.285 . 1 120 182 23 U H3' H 4.620 . 1 121 182 23 U H4' H 4.442 . 1 122 182 23 U H5 H 5.586 . 1 123 182 23 U H5' H 4.160 . 2 124 182 23 U H5'' H 4.362 . 2 125 182 23 U H6 H 7.668 . 1 126 183 24 U H1' H 5.923 . 1 127 183 24 U H2' H 4.425 . 1 128 183 24 U H3' H 4.566 . 1 129 183 24 U H4' H 4.410 . 1 130 183 24 U H5 H 5.857 . 1 131 183 24 U H5' H 4.115 . 2 132 183 24 U H5'' H 4.215 . 2 133 183 24 U H6 H 7.837 . 1 134 184 25 C H1' H 5.887 . 1 135 184 25 C H2' H 4.292 . 1 136 184 25 C H3' H 4.572 . 1 137 184 25 C H4' H 4.224 . 1 138 184 25 C H5 H 6.023 . 1 139 184 25 C H5' H 3.943 . 2 140 184 25 C H5'' H 4.017 . 2 141 184 25 C H6 H 7.740 . 1 142 185 26 G H1' H 5.764 . 1 143 185 26 G H2' H 4.775 . 1 144 185 26 G H3' H 4.885 . 1 145 185 26 G H4' H 4.405 . 1 146 185 26 G H5' H 4.120 . 2 147 185 26 G H5'' H 4.187 . 2 148 185 26 G H8 H 7.919 . 1 149 186 27 G H1' H 5.714 . 1 150 186 27 G H2' H 4.858 . 1 151 186 27 G H3' H 4.886 . 1 152 186 27 G H4' H 4.485 . 1 153 186 27 G H5' H 4.199 . 2 154 186 27 G H5'' H 4.287 . 2 155 186 27 G H8 H 7.905 . 1 156 187 28 G H1' H 5.978 . 1 157 187 28 G H2' H 4.693 . 1 158 187 28 G H3' H 4.964 . 1 159 187 28 G H4' H 4.483 . 1 160 187 28 G H5' H 4.332 . 2 161 187 28 G H5'' H 4.393 . 2 162 187 28 G H8 H 8.076 . 1 163 188 29 G H1' H 5.829 . 1 164 188 29 G H2' H 4.881 . 1 165 188 29 G H3' H 4.963 . 1 166 188 29 G H4' H 4.685 . 1 167 188 29 G H5' H 4.350 . 2 168 188 29 G H8 H 8.031 . 1 169 189 30 A H1' H 5.945 . 1 170 189 30 A H2 H 7.636 . 1 171 189 30 A H2' H 4.718 . 1 172 189 30 A H3' H 4.585 . 1 173 189 30 A H4' H 4.249 . 1 174 189 30 A H8 H 7.829 . 1 175 190 31 G H1' H 5.733 . 1 176 190 31 G H2' H 4.513 . 1 177 190 31 G H3' H 4.497 . 1 178 190 31 G H4' H 4.147 . 1 179 190 31 G H8 H 7.256 . 1 180 191 32 C H1' H 5.637 . 1 181 191 32 C H2' H 4.744 . 1 182 191 32 C H3' H 4.513 . 1 183 191 32 C H4' H 4.160 . 1 184 191 32 C H5 H 5.223 . 1 185 191 32 C H6 H 7.564 . 1 186 192 33 G H1' H 6.590 . 1 187 192 33 G H2' H 4.595 . 1 188 192 33 G H3' H 4.481 . 1 189 192 33 G H4' H 4.198 . 1 190 192 33 G H8 H 7.495 . 1 191 193 34 G H1' H 5.731 . 1 192 193 34 G H2' H 4.803 . 1 193 193 34 G H8 H 7.087 . 1 194 194 35 A H1' H 6.070 . 1 195 194 35 A H2 H 7.899 . 1 196 194 35 A H2' H 4.558 . 1 197 194 35 A H3' H 4.779 . 1 198 194 35 A H4' H 4.755 . 1 199 194 35 A H8 H 8.072 . 1 200 195 36 C H1' H 5.434 . 1 201 195 36 C H5 H 5.263 . 1 202 195 36 C H6 H 7.446 . 1 203 196 37 G H1' H 5.873 . 1 204 196 37 G H2' H 4.362 . 1 205 196 37 G H3' H 4.613 . 1 206 196 37 G H4' H 4.180 . 1 207 196 37 G H8 H 7.737 . 1 208 197 38 A H1' H 5.925 . 1 209 197 38 A H2 H 7.964 . 1 210 197 38 A H2' H 4.847 . 1 211 197 38 A H8 H 7.671 . 1 212 198 39 U H1' H 5.606 . 1 213 198 39 U H2' H 3.538 . 1 214 198 39 U H3' H 4.345 . 1 215 198 39 U H4' H 4.219 . 1 216 198 39 U H5 H 6.476 . 1 217 198 39 U H6 H 7.737 . 1 218 199 40 G H1' H 6.238 . 1 219 199 40 G H2' H 4.827 . 1 220 199 40 G H3' H 4.342 . 1 221 199 40 G H4' H 5.106 . 1 222 199 40 G H5' H 4.187 . 2 223 199 40 G H5'' H 4.511 . 2 224 199 40 G H8 H 7.952 . 1 225 200 41 A H1' H 6.320 . 1 226 200 41 A H2 H 8.548 . 1 227 200 41 A H2' H 5.136 . 1 228 200 41 A H3' H 5.092 . 1 229 200 41 A H4' H 4.229 . 1 230 200 41 A H5' H 4.400 . 2 231 200 41 A H5'' H 4.432 . 2 232 200 41 A H8 H 8.790 . 1 233 201 42 C H1' H 6.366 . 1 234 201 42 C H2' H 4.724 . 1 235 201 42 C H3' H 4.566 . 1 236 201 42 C H4' H 4.511 . 1 237 201 42 C H5 H 5.787 . 1 238 201 42 C H6 H 8.156 . 1 239 202 43 C H1' H 5.612 . 1 240 202 43 C H5 H 5.595 . 1 241 202 43 C H6 H 7.914 . 1 242 203 44 C H1' H 5.605 . 1 243 203 44 C H5 H 5.597 . 1 244 203 44 C H6 H 7.930 . 1 245 204 45 U H1' H 5.656 . 1 246 204 45 U H2' H 4.360 . 1 247 204 45 U H3' H 4.653 . 1 248 204 45 U H4' H 4.518 . 1 249 204 45 U H5 H 5.472 . 1 250 204 45 U H5' H 4.189 . 2 251 204 45 U H5'' H 4.185 . 2 252 204 45 U H6 H 7.800 . 1 253 205 46 A H1' H 5.717 . 1 254 205 46 A H2 H 7.977 . 1 255 205 46 A H2' H 4.478 . 1 256 205 46 A H3' H 4.709 . 1 257 205 46 A H4' H 4.186 . 1 258 205 46 A H5' H 4.206 . 2 259 205 46 A H8 H 8.112 . 1 260 206 47 G H1' H 5.434 . 1 261 206 47 G H2' H 4.719 . 1 262 206 47 G H3' H 4.650 . 1 263 206 47 G H4' H 4.194 . 1 264 206 47 G H5' H 4.069 . 2 265 206 47 G H5'' H 4.295 . 2 266 206 47 G H8 H 7.632 . 1 267 207 48 U H1' H 5.881 . 1 268 207 48 U H2' H 4.292 . 1 269 207 48 U H3' H 4.596 . 1 270 207 48 U H4' H 3.970 . 1 271 207 48 U H5 H 5.868 . 1 272 207 48 U H5' H 3.786 . 2 273 207 48 U H5'' H 3.839 . 2 274 207 48 U H6 H 7.697 . 1 275 208 49 A H1' H 5.881 . 1 276 208 49 A H2 H 7.329 . 1 277 208 49 A H2' H 4.836 . 1 278 208 49 A H3' H 4.903 . 1 279 208 49 A H4' H 4.479 . 1 280 208 49 A H5' H 4.268 . 2 281 208 49 A H5'' H 4.241 . 2 282 208 49 A H8 H 8.258 . 1 283 209 50 G H1' H 6.255 . 1 284 209 50 G H2' H 4.980 . 1 285 209 50 G H3' H 4.472 . 1 286 209 50 G H4' H 4.989 . 1 287 209 50 G H8 H 8.062 . 1 288 210 51 A H1' H 6.255 . 1 289 210 51 A H2 H 7.376 . 1 290 210 51 A H2' H 4.705 . 1 291 210 51 A H3' H 4.623 . 1 292 210 51 A H4' H 4.461 . 1 293 210 51 A H5' H 4.341 . 2 294 210 51 A H5'' H 4.282 . 2 295 210 51 A H8 H 8.254 . 1 296 211 52 G H1' H 5.775 . 1 297 211 52 G H8 H 6.796 . 1 298 212 53 G H1' H 5.881 . 1 299 212 53 G H8 H 7.288 . 1 300 213 54 G H1' H 5.942 . 1 301 213 54 G H2' H 4.810 . 1 302 213 54 G H3' H 4.671 . 1 303 213 54 G H5' H 4.528 . 2 304 213 54 G H5'' H 4.596 . 2 305 213 54 G H8 H 7.176 . 1 306 214 55 G H1' H 5.867 . 1 307 214 55 G H2' H 4.720 . 1 308 214 55 G H3' H 4.799 . 1 309 214 55 G H4' H 4.464 . 1 310 214 55 G H5' H 4.167 . 1 311 214 55 G H8 H 7.125 . 1 312 215 56 G H1' H 5.852 . 1 313 215 56 G H2' H 4.801 . 1 314 215 56 G H8 H 7.287 . 1 315 216 57 C H1' H 5.569 . 1 316 216 57 C H2' H 4.513 . 1 317 216 57 C H3' H 4.358 . 1 318 216 57 C H5 H 5.300 . 1 319 216 57 C H6 H 7.465 . 1 320 217 58 U H1' H 5.841 . 1 321 217 58 U H2' H 4.006 . 1 322 217 58 U H3' H 4.793 . 1 323 217 58 U H4' H 4.797 . 1 324 217 58 U H5 H 5.843 . 1 325 217 58 U H6 H 7.976 . 1 326 218 59 G H1' H 5.857 . 1 327 218 59 G H2' H 5.226 . 1 328 218 59 G H3' H 4.180 . 1 329 218 59 G H4' H 4.648 . 1 330 218 59 G H5' H 4.301 . 1 331 218 59 G H8 H 8.356 . 1 332 219 60 C H1' H 6.095 . 1 333 219 60 C H2' H 4.239 . 1 334 219 60 C H3' H 4.629 . 1 335 219 60 C H4' H 3.849 . 1 336 219 60 C H5 H 6.265 . 1 337 219 60 C H5' H 2.856 . 1 338 219 60 C H5'' H 3.739 . 1 339 219 60 C H6 H 7.866 . 1 340 220 61 G H1' H 6.073 . 1 341 220 61 G H2' H 4.896 . 1 342 220 61 G H3' H 5.775 . 1 343 220 61 G H8 H 8.038 . 1 344 221 62 G H1' H 4.904 . 1 345 221 62 G H2' H 4.284 . 1 346 221 62 G H3' H 4.315 . 1 347 221 62 G H4' H 4.439 . 1 348 221 62 G H5' H 4.511 . 1 349 221 62 G H5'' H 4.578 . 1 350 221 62 G H8 H 8.457 . 1 351 222 63 C H1' H 5.567 . 1 352 222 63 C H5 H 5.301 . 1 353 222 63 C H6 H 7.667 . 1 354 223 64 U H1' H 5.791 . 1 355 223 64 U H2' H 4.465 . 1 356 223 64 U H3' H 4.709 . 1 357 223 64 U H4' H 4.105 . 1 358 223 64 U H5 H 5.813 . 1 359 223 64 U H6 H 7.935 . 1 360 224 65 U H1' H 6.001 . 1 361 224 65 U H2' H 4.364 . 1 362 224 65 U H3' H 4.606 . 1 363 224 65 U H4' H 4.523 . 1 364 224 65 U H5 H 6.059 . 1 365 224 65 U H5' H 4.112 . 1 366 224 65 U H5'' H 4.187 . 1 367 224 65 U H6 H 7.915 . 1 368 225 66 A H1' H 6.088 . 1 369 225 66 A H2 H 8.243 . 1 370 225 66 A H2' H 4.662 . 1 371 225 66 A H3' H 4.906 . 1 372 225 66 A H4' H 4.724 . 1 373 225 66 A H5' H 4.227 . 2 374 225 66 A H5'' H 4.245 . 2 375 225 66 A H8 H 8.437 . 1 376 226 67 G H1' H 5.792 . 1 377 226 67 G H8 H 8.071 . 1 378 227 68 G H1' H 5.807 . 1 379 227 68 G H2' H 4.902 . 1 380 227 68 G H3' H 5.002 . 1 381 227 68 G H4' H 4.754 . 1 382 227 68 G H8 H 8.068 . 1 383 228 69 A H1' H 6.206 . 1 384 228 69 A H2 H 7.702 . 1 385 228 69 A H2' H 4.824 . 1 386 228 69 A H3' H 4.812 . 1 387 228 69 A H4' H 4.659 . 1 388 228 69 A H5'' H 4.417 . 2 389 228 69 A H8 H 8.120 . 1 390 229 70 G H1' H 5.797 . 1 391 229 70 G H2' H 4.643 . 1 392 229 70 G H3' H 4.576 . 1 393 229 70 G H4' H 4.198 . 1 394 229 70 G H8 H 7.246 . 1 395 230 71 G H1' H 5.849 . 1 396 230 71 G H8 H 7.231 . 1 397 231 72 G H1' H 5.596 . 1 398 231 72 G H8 H 7.295 . 1 399 232 73 C H1' H 5.596 . 1 400 232 73 C H5 H 5.320 . 1 401 232 73 C H6 H 7.697 . 1 402 233 74 A H1' H 5.981 . 1 403 233 74 A H2 H 7.145 . 1 404 233 74 A H2' H 4.705 . 1 405 233 74 A H3' H 4.602 . 1 406 233 74 A H4' H 4.248 . 1 407 233 74 A H5' H 4.369 . 1 408 233 74 A H8 H 8.062 . 1 409 234 75 G H1' H 5.763 . 1 410 234 75 G H8 H 7.359 . 1 stop_ save_ save_assigned_chem_shift_list_1_3 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D NOESY' 3D_13C-separated_NOESY stop_ loop_ _Sample_label $sample_1 $sample_2 $sample_3 $sample_4 $sample_5 $sample_6 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'NC protein' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 16 2 ARG HA H 4.526 . 1 2 16 2 ARG HB2 H 1.998 . 2 3 16 2 ARG HB3 H 1.864 . 2 4 16 2 ARG HD2 H 3.125 . 2 5 16 2 ARG HD3 H 3.189 . 2 6 16 2 ARG HG2 H 1.709 . 2 7 16 2 ARG HG3 H 1.779 . 2 8 16 2 ARG CA C 53.829 . 1 9 16 2 ARG CB C 29.218 . 1 10 16 2 ARG CD C 40.938 . 1 11 16 2 ARG CG C 24.767 . 1 12 17 3 ALA HA H 4.423 . 1 13 17 3 ALA HB H 1.442 . 1 14 17 3 ALA CA C 50.079 . 1 15 17 3 ALA CB C 17.032 . 1 16 19 5 GLY HA2 H 4.326 . 2 17 19 5 GLY HA3 H 4.009 . 2 18 19 5 GLY CA C 42.344 . 1 19 20 6 LEU HA H 4.671 . 1 20 20 6 LEU HB2 H 1.220 . 2 21 20 6 LEU HB3 H 1.220 . 2 22 20 6 LEU HD1 H 1.954 . 2 23 20 6 LEU HD2 H 0.859 . 2 24 20 6 LEU HG H 1.769 . 1 25 20 6 LEU CA C 51.017 . 1 26 20 6 LEU CB C 40.938 . 1 27 20 6 LEU CD1 C 22.657 . 1 28 20 6 LEU CD2 C 19.608 . 1 29 20 6 LEU CG C 25.533 . 1 30 21 7 CYS HA H 4.984 . 1 31 21 7 CYS HB2 H 3.052 . 1 32 21 7 CYS HB3 H 2.770 . 1 33 21 7 CYS CA C 57.577 . 1 34 21 7 CYS CB C 30.390 . 1 35 22 8 TYR HA H 4.907 . 1 36 22 8 TYR HB2 H 3.375 . 2 37 22 8 TYR HB3 H 3.909 . 2 38 22 8 TYR HD1 H 7.013 . 1 39 22 8 TYR HE1 H 6.450 . 1 40 22 8 TYR CA C 55.507 . 1 41 22 8 TYR CB C 35.781 . 1 42 22 8 TYR CD1 C 128.828 . 1 43 22 8 TYR CE1 C 115.467 . 1 44 23 9 THR HA H 4.530 . 1 45 23 9 THR HB H 4.762 . 1 46 23 9 THR HG2 H 1.347 . 1 47 23 9 THR CA C 63.802 . 1 48 23 9 THR CB C 66.953 . 1 49 23 9 THR CG2 C 21.017 . 1 50 24 10 CYS HA H 5.227 . 1 51 24 10 CYS HB2 H 3.523 . 1 52 24 10 CYS HB3 H 2.645 . 1 53 24 10 CYS CA C 56.639 . 1 54 24 10 CYS CB C 30.855 . 1 55 26 12 SER HA H 4.917 . 1 56 26 12 SER HB2 H 4.114 . 1 57 26 12 SER HB3 H 3.515 . 1 58 26 12 SER CA C 54.532 . 1 59 26 12 SER CB C 62.734 . 1 60 27 13 PRO HA H 4.963 . 1 61 27 13 PRO HB2 H 2.201 . 2 62 27 13 PRO HB3 H 2.136 . 2 63 27 13 PRO HD2 H 4.104 . 2 64 27 13 PRO HD3 H 3.962 . 2 65 27 13 PRO HG2 H 2.025 . 2 66 27 13 PRO HG3 H 2.025 . 2 67 27 13 PRO CA C 60.858 . 1 68 27 13 PRO CB C 29.686 . 1 69 27 13 PRO CD C 48.438 . 1 70 27 13 PRO CG C 24.298 . 1 71 28 14 GLY HA2 H 4.320 . 2 72 28 14 GLY HA3 H 4.010 . 2 73 29 15 HIS HA H 5.352 . 1 74 29 15 HIS HD2 H 7.870 . 1 75 29 15 HIS HE1 H 7.335 . 1 76 29 15 HIS CA C 49.610 . 1 77 29 15 HIS CD2 C 135.860 . 1 78 30 16 TYR HA H 4.623 . 1 79 30 16 TYR HB2 H 3.492 . 2 80 30 16 TYR HB3 H 2.997 . 2 81 30 16 TYR HD1 H 6.652 . 1 82 30 16 TYR HE1 H 6.826 . 1 83 30 16 TYR CA C 58.849 . 1 84 30 16 TYR CB C 38.125 . 1 85 30 16 TYR CD1 C 126.953 . 1 86 30 16 TYR CE1 C 115.467 . 1 87 31 17 GLN HA H 4.511 . 1 88 31 17 GLN HB2 H 2.645 . 2 89 31 17 GLN HB3 H 2.549 . 2 90 31 17 GLN HG2 H 3.072 . 2 91 31 17 GLN HG3 H 2.773 . 2 92 31 17 GLN CA C 57.317 . 1 93 31 17 GLN CB C 26.405 . 1 94 31 17 GLN CG C 30.390 . 1 95 32 18 ALA HA H 4.397 . 1 96 32 18 ALA HB H 1.662 . 1 97 32 18 ALA CA C 52.423 . 1 98 32 18 ALA CB C 16.563 . 1 99 33 19 GLN HA H 4.631 . 1 100 33 19 GLN HG2 H 2.541 . 2 101 33 19 GLN HG3 H 2.385 . 2 102 33 19 GLN CA C 52.189 . 1 103 33 19 GLN CG C 32.265 . 1 104 34 20 CYS HA H 4.556 . 1 105 34 20 CYS HB2 H 2.581 . 1 106 34 20 CYS HB3 H 2.436 . 1 107 34 20 CYS CA C 62.499 . 1 108 34 20 CYS CB C 27.000 . 1 109 36 22 LYS HA H 4.367 . 1 110 36 22 LYS HB2 H 2.109 . 2 111 36 22 LYS HB3 H 1.987 . 2 112 36 22 LYS HD2 H 1.859 . 2 113 36 22 LYS HD3 H 1.838 . 2 114 36 22 LYS HE2 H 3.164 . 2 115 36 22 LYS HE3 H 3.164 . 2 116 36 22 LYS HG2 H 1.628 . 2 117 36 22 LYS HG3 H 1.584 . 2 118 36 22 LYS CA C 55.060 . 1 119 36 22 LYS CB C 29.970 . 1 120 36 22 LYS CD C 52.639 . 1 121 36 22 LYS CE C 39.625 . 1 122 36 22 LYS CG C 22.892 . 1 123 37 23 LYS HA H 4.406 . 1 124 37 23 LYS HB2 H 2.242 . 2 125 37 23 LYS HB3 H 2.110 . 2 126 37 23 LYS HD2 H 1.837 . 2 127 37 23 LYS HD3 H 1.837 . 2 128 37 23 LYS HE2 H 3.215 . 2 129 37 23 LYS HE3 H 3.215 . 2 130 37 23 LYS HG2 H 1.710 . 2 131 37 23 LYS HG3 H 1.710 . 2 132 37 23 LYS CA C 55.467 . 1 133 37 23 LYS CB C 29.452 . 1 134 37 23 LYS CD C 26.639 . 1 135 37 23 LYS CE C 39.766 . 1 136 37 23 LYS CG C 24.610 . 1 137 47 33 CYS HA H 3.695 . 1 138 47 33 CYS HB2 H 2.468 . 1 139 47 33 CYS HB3 H 2.468 . 1 140 47 33 CYS CA C 57.811 . 1 141 47 33 CYS CB C 27.108 . 1 142 48 34 GLN HA H 4.085 . 1 143 48 34 GLN HB2 H 2.239 . 2 144 48 34 GLN HB3 H 1.975 . 2 145 48 34 GLN HG2 H 2.560 . 2 146 48 34 GLN HG3 H 2.541 . 2 147 48 34 GLN CA C 55.702 . 1 148 48 34 GLN CB C 26.874 . 1 149 48 34 GLN CG C 32.514 . 1 150 49 35 LEU HA H 4.478 . 1 151 49 35 LEU HB2 H 1.858 . 2 152 49 35 LEU HB3 H 1.706 . 2 153 49 35 LEU HD1 H 0.997 . 1 154 49 35 LEU HD2 H 0.893 . 1 155 49 35 LEU HG H 1.568 . 1 156 49 35 LEU CA C 54.626 . 1 157 49 35 LEU CB C 39.755 . 1 158 49 35 LEU CD1 C 23.126 . 1 159 49 35 LEU CD2 C 20.782 . 1 160 49 35 LEU CG C 24.766 . 1 161 50 36 CYS HA H 4.589 . 1 162 50 36 CYS HB2 H 2.191 . 1 163 50 36 CYS HB3 H 2.129 . 1 164 50 36 CYS CA C 58.202 . 1 165 50 36 CYS CB C 31.798 . 1 166 51 37 ASN HA H 3.116 . 1 167 51 37 ASN HB2 H 3.276 . 2 168 51 37 ASN HB3 H 2.700 . 2 169 51 37 ASN CA C 53.326 . 1 170 51 37 ASN CB C 37.422 . 1 171 52 38 GLY HA2 H 3.708 . 2 172 52 38 GLY HA3 H 4.287 . 2 173 55 41 HIS HD2 H 6.385 . 1 174 55 41 HIS HE1 H 6.696 . 1 175 55 41 HIS CD2 C 124.843 . 1 176 55 41 HIS CE1 C 136.329 . 1 177 56 42 ASN HA H 4.707 . 1 178 56 42 ASN HB2 H 3.159 . 2 179 56 42 ASN HB3 H 2.63 . 2 180 56 42 ASN CA C 47.970 . 1 181 56 42 ASN CB C 39.230 . 1 182 57 43 ALA HA H 4.386 . 1 183 57 43 ALA HB H 1.417 . 1 184 57 43 ALA CA C 53.126 . 1 185 57 43 ALA CB C 17.010 . 1 186 58 44 LYS HA H 4.018 . 1 187 58 44 LYS HB2 H 1.839 . 2 188 58 44 LYS HB3 H 1.839 . 2 189 58 44 LYS HD2 H 1.663 . 2 190 58 44 LYS HD3 H 1.663 . 2 191 58 44 LYS HE2 H 2.960 . 2 192 58 44 LYS HE3 H 2.960 . 2 193 58 44 LYS HG2 H 1.415 . 2 194 58 44 LYS HG3 H 1.415 . 2 195 58 44 LYS CA C 52.423 . 1 196 58 44 LYS CB C 29.686 . 1 197 58 44 LYS CD C 27.108 . 1 198 58 44 LYS CE C 39.531 . 1 199 58 44 LYS CG C 22.892 . 1 200 60 46 CYS HA H 4.089 . 1 201 60 46 CYS HB2 H 3.344 . 1 202 60 46 CYS HB3 H 2.932 . 1 203 61 47 ARG HA H 4.450 . 1 204 61 47 ARG HB2 H 1.905 . 2 205 62 48 LYS HA H 4.440 . 1 206 62 48 LYS HB2 H 2.003 . 2 207 62 48 LYS HB3 H 2.003 . 2 208 62 48 LYS HD2 H 1.717 . 2 209 62 48 LYS HD3 H 1.717 . 2 210 62 48 LYS HE2 H 3.103 . 2 211 62 48 LYS HE3 H 3.103 . 2 212 62 48 LYS HG2 H 1.507 . 2 213 62 48 LYS HG3 H 1.507 . 2 214 62 48 LYS CA C 53.361 . 1 215 62 48 LYS CB C 31.327 . 1 216 62 48 LYS CD C 25.913 . 1 217 62 48 LYS CE C 40.234 . 1 218 62 48 LYS CG C 23.595 . 1 219 63 49 ARG HA H 4.220 . 1 220 63 49 ARG HB2 H 1.888 . 2 221 63 49 ARG HB3 H 1.847 . 2 222 63 49 ARG HD2 H 3.276 . 2 223 63 49 ARG HD3 H 3.276 . 2 224 63 49 ARG HG2 H 1.719 . 2 225 63 49 ARG HG3 H 1.602 . 2 226 63 49 ARG CA C 54.533 . 1 227 63 49 ARG CB C 28.514 . 1 228 63 49 ARG CD C 41.172 . 1 229 63 49 ARG CG C 24.767 . 1 stop_ save_