Sequence for Human DHFR:

             1   VAL     2   GLY     3   SER     4   LEU     5   ASN 
             6   CYS     7   ILE     8   VAL     9   ALA    10   VAL 
            11   SER    12   GLN    13   ASN    14   MET    15   GLY 
            16   ILE    17   GLY    18   LYS    19   ASN    20   GLY 
            21   ASP    22   LEU    23   PRO    24   TRP    25   PRO 
            26   PRO    27   LEU    28   ARG    29   ASN    30   GLU 
            31   PHE    32   ARG    33   TYR    34   PHE    35   GLN 
            36   ARG    37   MET    38   THR    39   THR    40   THR 
            41   SER    42   SER    43   VAL    44   GLU    45   GLY 
            46   LYS    47   GLN    48   ASN    49   LEU    50   VAL 
            51   ILE    52   MET    53   GLY    54   LYS    55   LYS 
            56   THR    57   TRP    58   PHE    59   SER    60   ILE 
            61   PRO    62   GLU    63   LYS    64   ASN    65   ARG 
            66   PRO    67   LEU    68   LYS    69   GLY    70   ARG 
            71   ILE    72   ASN    73   LEU    74   VAL    75   LEU 
            76   SER    77   ARG    78   GLU    79   LEU    80   LYS 
            81   GLU    82   PRO    83   PRO    84   GLN    85   GLY 
            86   ALA    87   HIS    88   PHE    89   LEU    90   SER 
            91   ARG    92   SER    93   LEU    94   ASP    95   ASP 
            96   ALA    97   LEU    98   LYS    99   LEU   100   THR 
           101   GLU   102   GLN   103   PRO   104   GLU   105   LEU 
           106   ALA   107   ASN   108   LYS   109   VAL   110   ASP 
           111   MET   112   VAL   113   TRP   114   ILE   115   VAL 
           116   GLY   117   GLY   118   SER   119   SER   120   VAL 
           121   TYR   122   LYS   123   GLU   124   ALA   125   MET 
           126   ASN   127   HIS   128   PRO   129   GLY   130   HIS 
           131   LEU   132   LYS   133   LEU   134   PHE   135   VAL 
           136   THR   137   ARG   138   ILE   139   MET   140   GLN 
           141   ASP   142   PHE   143   GLU   144   SER   145   ASP 
           146   THR   147   PHE   148   PHE   149   PRO   150   GLU 
           151   ILE   152   ASP   153   LEU   154   GLU   155   LYS 
           156   TYR   157   LYS   158   LEU   159   LEU   160   PRO 
           161   GLU   162   TYR   163   PRO   164   GLY   165   VAL 
           166   LEU   167   SER   168   ASP   169   VAL   170   GLN 
           171   GLU   172   GLU   173   LYS   174   GLY   175   ILE 
           176   LYS   177   TYR   178   LYS   179   PHE   180   GLU 
           181   VAL   182   TYR   183   GLU   184   LYS   185   ASN 
           186   ASP 
      
   Formula Weight for Human DHFR:  21322 Da
   
   
   
Human           VGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSI 60
Chicken         VRSLNSIVAVCQNMGIGKDGNLPWPPLRNEYKYFQRMTSTSHVEGKQNAVIMGKKTWFSI 60
Drosophila      MLRFNLIVAVCENFGIGIRGDLPWR-IKSELKYFSRTTKRTSDPTKQNAVVMGRKTYFGV 59
E. coli         --MISLIAALAVDRVIGMENAMPWN-LPADLAWFKRNTLN-------KPVIMGRHTWESI 50
                   :. *.*:. :  **  . :**  :  :  :*.* *         : *:**::*: .:

Human     	PEKNRPLKGRINLVLSRELKEP--PQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGS 118
Chicken   	PEKNRPLKDRINIVLSRELKEA--PKGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGT 118
Drosophila	PESKRPLPDRLNIVLSTTLQESDLPKG-VLLCPNLETAMKILEE---QNEVENIWIVGGS 115
E. coli   	G---RPLPGRKNIILSSQPGTD----DRVTWVKSVDEAIAACGD------VPEIMVIGGG 97
                    *** .* *::**          .      .:: *:    .      *  : ::** 

Human     	SVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYK 178
Chicken   	AVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADIQEEDGIQYK 178
Drosophila	GVYEEAMASPRCHRLYITKIMQKFDCDTFFPAIP-DSFREVAPDSDMPLGVQEENGIKFE 174
E. coli   	RVYEQFLPK--AQKLYLTHIDAEVEGDTHFPDYEPDDWESVFS---EFHDADAQNSHSYC 152
                 **:  :      :*::*:*  ..: **.**    ..:. :        . : :.. .: 

Human     	FEVYEKND--- 186
Chicken   	FEVYQKSVLAQ 189
Drosophila	YKILEKHS--- 182
E. coli   	FEILERR---- 159
                ::: ::