Content for NMR-STAR saveframe, "heteronuclear_T2_list_E_State"
save_heteronuclear_T2_list_E_State
_Heteronucl_T2_list.Sf_category heteronucl_T2_relaxation
_Heteronucl_T2_list.Sf_framecode heteronuclear_T2_list_E_State
_Heteronucl_T2_list.Entry_ID 26519
_Heteronucl_T2_list.ID 2
_Heteronucl_T2_list.Sample_condition_list_ID 1
_Heteronucl_T2_list.Sample_condition_list_label $sample_conditions_1
_Heteronucl_T2_list.Temp_calibration_method 'no calibration applied'
_Heteronucl_T2_list.Temp_control_method 'no temperature control applied'
_Heteronucl_T2_list.Spectrometer_frequency_1H 500
_Heteronucl_T2_list.T2_coherence_type S(+,-)
_Heteronucl_T2_list.T2_val_units s-1
_Heteronucl_T2_list.Rex_units .
_Heteronucl_T2_list.Details
;
Excited State
Values of obtained from fits of all CEST profiles recorded on the
Abp1p-Ark1p system, 1C, 11.7T to a global two-site exchange processes. Values of (kex,pE)
were fixed to (130 s-1, 2.38%) and profiles for all of the residues fit simultaneously.
The following six residues showed clear evidence for a second state (well resolved dips for both ground and excited states in
profiles):
15
31
32
33
34
37
;
_Heteronucl_T2_list.Text_data_format .
_Heteronucl_T2_list.Text_data .
loop_
_Heteronucl_T2_experiment.Experiment_ID
_Heteronucl_T2_experiment.Experiment_name
_Heteronucl_T2_experiment.Sample_ID
_Heteronucl_T2_experiment.Sample_label
_Heteronucl_T2_experiment.Sample_state
_Heteronucl_T2_experiment.Entry_ID
_Heteronucl_T2_experiment.Heteronucl_T2_list_ID
1 '2D 1H-15N HSQC 1' . . . 26519 2
2 '2D 1H-15N HSQC 2' . . . 26519 2
3 '2D 1H-15N HMQC 1' . . . 26519 2
4 '2D 1H-15N HMQC 2' . . . 26519 2
stop_
loop_
_T2.ID
_T2.Assembly_atom_ID
_T2.Entity_assembly_ID
_T2.Entity_ID
_T2.Comp_index_ID
_T2.Seq_ID
_T2.Comp_ID
_T2.Atom_ID
_T2.Atom_type
_T2.Atom_isotope_number
_T2.T2_val
_T2.T2_val_err
_T2.Rex_val
_T2.Rex_err
_T2.Resonance_ID
_T2.Auth_entity_assembly_ID
_T2.Auth_seq_ID
_T2.Auth_comp_ID
_T2.Auth_atom_ID
_T2.Entry_ID
_T2.Heteronucl_T2_list_ID
1 . 1 1 1 1 ALA N N 15 0.7 10.2 . . . . . . . 26519 2
2 . 1 1 3 3 TRP N N 15 4.8 14.5 . . . . . . . 26519 2
3 . 1 1 4 4 ALA N N 15 2.6 15.5 . . . . . . . 26519 2
4 . 1 1 5 5 THR N N 15 16.4 11.8 . . . . . . . 26519 2
5 . 1 1 6 6 ALA N N 15 94.7 28.5 . . . . . . . 26519 2
6 . 1 1 7 7 GLU N N 15 12.3 60.4 . . . . . . . 26519 2
7 . 1 1 8 8 TYR N N 15 5.7 8.5 . . . . . . . 26519 2
8 . 1 1 9 9 ASP N N 15 0.1 8.8 . . . . . . . 26519 2
9 . 1 1 10 10 TYR N N 15 19.4 9.5 . . . . . . . 26519 2
10 . 1 1 11 11 ASP N N 15 3.8 8.7 . . . . . . . 26519 2
11 . 1 1 12 12 ALA N N 15 2.4 13.4 . . . . . . . 26519 2
12 . 1 1 13 13 ALA N N 15 10.9 9.8 . . . . . . . 26519 2
13 . 1 1 14 14 GLU N N 15 3.2 8.1 . . . . . . . 26519 2
14 . 1 1 15 15 ASP N N 15 13.4 3.6 . . . . . . . 26519 2
15 . 1 1 16 16 ASN N N 15 18.6 7.7 . . . . . . . 26519 2
16 . 1 1 17 17 GLU N N 15 7.1 4.5 . . . . . . . 26519 2
17 . 1 1 18 18 LEU N N 15 1.3 12.2 . . . . . . . 26519 2
18 . 1 1 19 19 THR N N 15 4.8 7.6 . . . . . . . 26519 2
19 . 1 1 20 20 PHE N N 15 17.1 28.3 . . . . . . . 26519 2
20 . 1 1 21 21 VAL N N 15 2.9 24.9 . . . . . . . 26519 2
21 . 1 1 22 22 GLU N N 15 58.4 21.5 . . . . . . . 26519 2
22 . 1 1 23 23 ASN N N 15 15.2 15.7 . . . . . . . 26519 2
23 . 1 1 24 24 ASP N N 15 7.4 16.3 . . . . . . . 26519 2
24 . 1 1 25 25 LYS N N 15 2.6 14.8 . . . . . . . 26519 2
25 . 1 1 27 27 ILE N N 15 39.7 38.3 . . . . . . . 26519 2
26 . 1 1 28 28 ASN N N 15 47.8 28.9 . . . . . . . 26519 2
27 . 1 1 29 29 ILE N N 15 22.7 110 . . . . . . . 26519 2
28 . 1 1 30 30 GLU N N 15 3.0 7.7 . . . . . . . 26519 2
29 . 1 1 31 31 PHE N N 15 6.3 4.2 . . . . . . . 26519 2
30 . 1 1 32 32 VAL N N 15 19.9 5.2 . . . . . . . 26519 2
31 . 1 1 33 33 ASP N N 15 13.1 6.9 . . . . . . . 26519 2
32 . 1 1 34 34 ASP N N 15 27.7 4.4 . . . . . . . 26519 2
33 . 1 1 35 35 ASP N N 15 22.2 6.0 . . . . . . . 26519 2
34 . 1 1 36 36 TRP N N 15 12.8 8.0 . . . . . . . 26519 2
35 . 1 1 37 37 TRP N N 15 4.0 8.8 . . . . . . . 26519 2
36 . 1 1 38 38 LEU N N 15 2.5 19.4 . . . . . . . 26519 2
37 . 1 1 39 39 GLY N N 15 18.1 13.8 . . . . . . . 26519 2
38 . 1 1 40 40 GLU N N 15 0.8 5.5 . . . . . . . 26519 2
39 . 1 1 41 41 LEU N N 15 2.4 13.3 . . . . . . . 26519 2
40 . 1 1 42 42 GLU N N 15 0.2 10.1 . . . . . . . 26519 2
41 . 1 1 43 43 LYS N N 15 18.4 33.0 . . . . . . . 26519 2
42 . 1 1 44 44 ASP N N 15 1.5 11.2 . . . . . . . 26519 2
43 . 1 1 45 45 GLY N N 15 0.6 18.4 . . . . . . . 26519 2
44 . 1 1 46 46 SER N N 15 25.1 20.2 . . . . . . . 26519 2
45 . 1 1 47 47 LYS N N 15 66.9 20.6 . . . . . . . 26519 2
46 . 1 1 48 48 GLY N N 15 23.8 31.6 . . . . . . . 26519 2
47 . 1 1 49 49 LEU N N 15 15.9 8.5 . . . . . . . 26519 2
48 . 1 1 50 50 PHE N N 15 1.2 27.9 . . . . . . . 26519 2
49 . 1 1 52 52 SER N N 15 44.7 9.1 . . . . . . . 26519 2
50 . 1 1 53 53 ASN N N 15 0.4 10.1 . . . . . . . 26519 2
51 . 1 1 54 54 TYR N N 15 5.9 26.8 . . . . . . . 26519 2
52 . 1 1 55 55 VAL N N 15 176.8 26.0 . . . . . . . 26519 2
53 . 1 1 56 56 SER N N 15 35.4 20.0 . . . . . . . 26519 2
54 . 1 1 57 57 LEU N N 15 39.7 19.0 . . . . . . . 26519 2
55 . 1 1 58 58 GLY N N 15 2.2 18.0 . . . . . . . 26519 2
56 . 1 1 59 59 ASN N N 15 0.5 14.9 . . . . . . . 26519 2
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