BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7421

Title: STRUCTURE OF CCP MODULE 7 OF COMPLEMENT FACTOR H- THE AMD NOT AT RISK VARIENT (402Y)   PubMed: 17360715

Authors: Herbert, A.; Deakin, J.; Schmidt, C.; Blaum, B.; Egan, C.; Ferreira, V.; Pangburn, M.; Lyon, M.; Uhrin, D.; Barlow, P.

Citation: Herbert, A.; Deakin, J.; Schmidt, C.; Blaum, B.; Egan, C.; Ferreira, V.; Pangburn, M.; Lyon, M.; Uhrin, D.; Barlow, P.. "Structure Shows Glycosaminoglycan- and Protein-Recognition Site in Factor H is Perturbed by Age-Related Macular Degeneration-Linked single nucleotide polymorphism"  J. Biol. Chem. 282, 18960-18968 (2007).

Assembly members:
COMPLEMENT_FACTOR_H, polymer, 65 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: PICHIA PASTORIS

Entity Sequences (FASTA):
COMPLEMENT_FACTOR_H: EAAGLRKCYFPYLENGYNQN YGRKFVQGKSIDVACHPGYA LPKAQTTVTCMENGWSPTPR CIRVK

Data sets:
Data typeCount
13C chemical shifts282
15N chemical shifts68
1H chemical shifts444

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1COMPLEMENT FACTOR H1

Entities:

Entity 1, COMPLEMENT FACTOR H 65 residues - Formula weight is not available

1   GLUALAALAGLYLEUARGLYSCYSTYRPHE
2   PROTYRLEUGLUASNGLYTYRASNGLNASN
3   TYRGLYARGLYSPHEVALGLNGLYLYSSER
4   ILEASPVALALACYSHISPROGLYTYRALA
5   LEUPROLYSALAGLNTHRTHRVALTHRCYS
6   METGLUASNGLYTRPSERPROTHRPROARG
7   CYSILEARGVALLYS

Samples:

sample: COMPLEMENT FACTOR H mM

sample_conditions_1: ionic strength: 20 mM; pH: 5.2; pressure: 1.0 atm; temperature: 298.0 K

Experiments:

NameSampleSample stateSample conditions
CBCANHsampleisotropicsample_conditions_1
H_CCONH TOCSYsampleisotropicsample_conditions_1
HNCOsampleisotropicsample_conditions_1
15N EDITED TOCSY HSQCsampleisotropicsample_conditions_1
13C EDITED NOESY HSQCsampleisotropicsample_conditions_1
15N EDITED NOESY HSQCsampleisotropicsample_conditions_1
HCCH TOCSYsampleisotropicsample_conditions_1
HBCBCGCDCEHEsampleisotropicsample_conditions_1
HBCBCGCDHDsampleisotropicsample_conditions_1
C_CCONH TOCSYsampleisotropicsample_conditions_1

Software:

CNS v1.1, BRUNGER,ADAMS,CLORE,DELANO,GROS, GROSSE-KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,READ,RICE,SIMONSON,WARREN - refinement

ANALYSIS vANALYSIS - structure solution

NMR spectrometers:

  • BRUKER OTHER 600 MHz

Related Database Links:

BMRB 7420
PDB
EMBL CAA30403 CAB41739
GB AAA52013 AAH37285 AAI10644 ADZ15369 AHH32464
REF NP_001014975 XP_004028137