BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7396

Title: Solution Structure of ETO-TAFH refined in explicit solvent   PubMed: 17572682

Authors: Wei, Y.; Liu, S.; Lausen, J.; Woodrell, C.; Cho, S.; Biris, N.; Kobayashi, N.; Yokoyama, S.; Werner, M.

Citation: Wei, Y.; Liu, S.; Lausen, J.; Woodrell, C.; Cho, S.; Biris, N.; Kobayashi, N.; Wei, Y.; Yokoyama, S.; Werner, M.. "A TAF4-homology domain from the corepressor ETO is a docking platform for positive and negative regulators of transcription"  Nat. Struct. Mol. Biol. 14, 653-661 (2007).

Assembly members:
Protein_ETO, polymer, 107 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Protein_ETO: GARQLSKLKRFLTTLQQYGN DISPEIGERVRTLVLGLVNS TLTIEEFHSKLQEATNFPLR PFVIPFLKANLPLLQRELLH CARLAKQNPAQYLAQHEQLL LDASTTS

Data sets:
Data typeCount
13C chemical shifts388
15N chemical shifts111
1H chemical shifts802

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ETO1

Entities:

Entity 1, ETO 107 residues - Formula weight is not available

1   GLYALAARGGLNLEUSERLYSLEULYSARG
2   PHELEUTHRTHRLEUGLNGLNTYRGLYASN
3   ASPILESERPROGLUILEGLYGLUARGVAL
4   ARGTHRLEUVALLEUGLYLEUVALASNSER
5   THRLEUTHRILEGLUGLUPHEHISSERLYS
6   LEUGLNGLUALATHRASNPHEPROLEUARG
7   PROPHEVALILEPROPHELEULYSALAASN
8   LEUPROLEULEUGLNARGGLULEULEUHIS
9   CYSALAARGLEUALALYSGLNASNPROALA
10   GLNTYRLEUALAGLNHISGLUGLNLEULEU
11   LEUASPALASERTHRTHRSER

Samples:

sample: ETO 1.1 mM; phosphate buffer 200 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 6.8; pressure: 1 .; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsampleisotropicsample_conditions_1
3D_13C-separated_NOESYsampleisotropicsample_conditions_1

Software:

X-PLOR NIH v2.14, Schwieters, C.D. - refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz
  • Bruker AVANCE 900 MHz

Related Database Links:

BMRB 16467 7147
PDB
DBJ BAA03089 BAA03247 BAA03558 BAA03559 BAA03560
EMBL CAA56311 CAF99400 CAG33073
GB AAB34819 AAB34820 AAC28931 AAC28932 AAC28933
REF NP_001070244 NP_001089065 NP_001102127 NP_001104496 NP_001104497
SP Q06455 Q61909