BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7366

Title: Solution Structure of Putative periplasmic protein: Northest Structural Genomics Target StR106

Authors: Zhang, Q.; Liu, G.; Wang, H.; Nwosu, C.; Cunningham, K.; Ma, L.; Xiao, R.; Liu, J.; Baran, M.; Swapna, G.; Thomas, B.; Rost, B.; Montelione, G.; Szypersk, T.

Citation: Zhang, Q.; Liu, G.; Wang, H.; Nwosu, C.; Cunningham, K.; Ma, L.; Xiao, R.; Liu, J.; Baran, M.; Swapna, G.; Thomas, B.; Montelione, G.; Szypersk, T.. "Solution Structure of Putative periplasmic protein: Northest Structural Genomics Target StR106"  To be Published ., .-..

Assembly members:
Putative_periplasmic_protein, polymer, 99 residues, Formula weight is not available

Natural source:   Common Name: Salmonella choleraesuis   Taxonomy ID: 591   Superkingdom: not available   Kingdom: Bacteria   Genus/species: Salmonella Salmonella

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Putative_periplasmic_protein: MKTGYKVMLGALAFVVTNVY AAEIMKKTDFDKVASEYTKI GTISTTGEMSPLDAREDLIK KADEKGADVVVLTSGQTENK IHGTADIYKKKLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts261
15N chemical shifts77
1H chemical shifts536

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Periplasmic protein1

Entities:

Entity 1, Periplasmic protein 99 residues - Formula weight is not available

1   METLYSTHRGLYTYRLYSVALMETLEUGLY
2   ALALEUALAPHEVALVALTHRASNVALTYR
3   ALAALAGLUILEMETLYSLYSTHRASPPHE
4   ASPLYSVALALASERGLUTYRTHRLYSILE
5   GLYTHRILESERTHRTHRGLYGLUMETSER
6   PROLEUASPALAARGGLUASPLEUILELYS
7   LYSALAASPGLULYSGLYALAASPVALVAL
8   VALLEUTHRSERGLYGLNTHRGLUASNLYS
9   ILEHISGLYTHRALAASPILETYRLYSLYS
10   LYSLEUGLUHISHISHISHISHISHIS

Samples:

sample: Periplasmic protein 1.1 mM; Tris 10 mM; NaCl 100 mM; NaN3 0.02%; D2O 10%; H2O 90%

sample_conditions_1: pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
GFT (4,3)D HNNCABCAsampleisotropicsample_conditions_1
GFT (4,3)D CABCA(CO)NHNsampleisotropicsample_conditions_1
GFT (4,3)D HABCAB(CO)NHNsampleisotropicsample_conditions_1
GFT (4,3)D HCCHsampleisotropicsample_conditions_1
SIMULTANEOUS HETERONUCLEAR RESOLVED [1H,1H]-NOESYsampleisotropicsample_conditions_1

Software:

DYANA v1.5, Guntert, P. et al. - data analysis

NMRPipe v2.3, Delaglio, F. et al. - processing

CYANA v2.1, Guntert, P. et al. - structure solution

CNS v1.1, Brunger, A.T. et al. - refinement

XEASY v1.3, Bartels, C., Xia, T.H. - data analysis

AutoStruct v2.0.0, Huang, Y.J. - data analysis

UBNMR v1.0, Shen, Y. - data analysis

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

BMRB 19327
PDB
DBJ BAJ35371 BAP06126
EMBL CAC12674 CAD08821 CAR31935 CAR36277 CAR60410
GB AAL19320 AAO70086 AAV78241 AAX64313 ABX68592
PIR AE0547
REF NP_454961 NP_459361 NP_806226 WP_000848022 WP_000848024