BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 7340

Title: NMR assignments for a helical diacylglycerol kinase (DAGK), 40 kDa membrane protein from E. coli   PubMed: 15099070

Authors: Kim, Hak Jun; Oxenoid, Kirill; Jacob, Jaison; Sonnichsen, Frank; Sanders, Chuck

Citation: Oxenoid, Kirill; Kim, Hak Jun; Jacob, Jaison; Sonnichsen, Frank; Sanders, Chuck. "NMR assignments for a helical 40 kDa membrane protein"  J. Am. Chem. Soc. 126, 5048-5049 (2004).

Assembly members:
DAGK, polymer, 122 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DAGK: MANNTTGFTRIIKAAGYSWK GLRAAWINEAAFRQEGVAVL LAVVIACWLDVDACTRVLLI SSVMLVMIVELLNSAIEAVV DRIGSEYHELSGRAKDMGSA AVLIAIIDAVITWCILLWSH FG

Data sets:
Data typeCount
13C chemical shifts252
15N chemical shifts86
1H chemical shifts86

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DAGK monomer 11
2DAGK monomer 21
3DAGK monomer 31

Entities:

Entity 1, DAGK monomer 1 122 residues - Formula weight is not available

1   METALAASNASNTHRTHRGLYPHETHRARG
2   ILEILELYSALAALAGLYTYRSERTRPLYS
3   GLYLEUARGALAALATRPILEASNGLUALA
4   ALAPHEARGGLNGLUGLYVALALAVALLEU
5   LEUALAVALVALILEALACYSTRPLEUASP
6   VALASPALACYSTHRARGVALLEULEUILE
7   SERSERVALMETLEUVALMETILEVALGLU
8   LEULEUASNSERALAILEGLUALAVALVAL
9   ASPARGILEGLYSERGLUTYRHISGLULEU
10   SERGLYARGALALYSASPMETGLYSERALA
11   ALAVALLEUILEALAILEILEASPALAVAL
12   ILETHRTRPCYSILELEULEUTRPSERHIS
13   PHEGLY

Samples:

sample_1: DAGK, [U-13C; U-15N; U-2H], 3 mM

sample_conditions_1: pH: 6.5; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

NMRPipe, F Delaglio, S Grzesiek, GW Vuister, G Zhu, J Pfeifer and A Bax - processing

NMRView, B Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 15019 19754
PDB
DBJ BAB38448 BAE78044 BAG79858 BAI28303 BAI33480
EMBL CAQ34391 CAR01021 CAR20569 CAV01327 CBG37235
GB AAA24394 AAC43136 AAC77012 AAG59241 AAN45585
REF NP_290676 NP_313052 NP_418466 NP_709878 NP_756865
SP P0ABN1 P0ABN2 P0ABN3 P0ABN4