BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7288

Title: 1H, 13C, 15N Chemical Shift Assignments and 15N Relaxation Data for Transmembrane Domain of BNIP3   PubMed: 17412696

Authors: Pustovalova, Julia; Bocharov, Eduard; Maslennikov, Innokenty; Ermolyuk, Yaroslav; Goncharuk, Marina; Arseniev, Alexander

Citation: Bocharov, Eduard; Pustovalova, Yulia; Pavlov, Konstantin; Volynsky, Pavel; Goncharuk, Marina; Ermolyuk, Yaroslav; Karpunin, Dmitry; Schulga, Alexey; Kirpichnikov, Michail; Efremov, Roman; Maslennikov, Innokenty; Arseniev, Alexander. "Unique dimeric structure of BNip3 transmembrane domain suggests membrane permeabilization as a cell death trigger."  J. Biol. Chem. 282, 16256-16266 (2007).

Assembly members:
BNIP3 TM, polymer, 45 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
BNIP3 TM: RNTSVMKKGGIFSAEFLKVF LPSLLLSHLLAIGLGIYIGR RLTTS

Data sets:
Data typeCount
13C chemical shifts114
15N chemical shifts45
1H chemical shifts323
heteronuclear NOE values38
H exchange rates44
T1 relaxation values38
T2 relaxation values38

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer 11
2monomer 21

Entities:

Entity 1, monomer 1 45 residues - Formula weight is not available

1   ARGASNTHRSERVALMETLYSLYSGLYGLY
2   ILEPHESERALAGLUPHELEULYSVALPHE
3   LEUPROSERLEULEULEUSERHISLEULEU
4   ALAILEGLYLEUGLYILETYRILEGLYARG
5   ARGLEUTHRTHRSER

Samples:

cn_labelled: BNIP3 TM, [U-95% 13C; U-95% 15N], 1.38 ± 0.02 mM; DHPC 44.13 ± 0.02 mM; DMPC 11.03 ± 0.02 mM; Acetic buffer 20 mM; NaN3 1.5 mM; EDTA 1 mM

n_labelled: BNIP3 TM, [U-95% 15N], 1.38 ± 0.02 mM; DHPC 44.13 ± 0.02 mM; DMPC 11.03 ± 0.02 mM; Acetic buffer 20 mM; NaN3 1.5 mM; EDTA 1 mM

heterodimer: BNIP3 TM, [U-95% 13C; U-95% 15N], 0.69 ± 0.02 mM; DHPC 44.13 ± 0.02 mM; DMPC 11.03 ± 0.02 mM; Acetic buffer 20 mM; NaN3 1.5 mM; EDTA 1 mM

conditions_1: pH: 5.10; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCnot availablenot availableconditions_1
3D 15N-NOESYnot availablenot availableconditions_1
1H13C HSQCnot availablenot availableconditions_1
3D 13C-NOESYnot availablenot availableconditions_1
HCCH-TOCSYnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian UNITY 600 MHz

Related Database Links:

BMRB 16012
PDB
DBJ BAB29214 BAC36072 BAD96346 BAE31356 BAG58159
EMBL CAX36485
GB AAC00022 AAC16738 AAD02922 AAF98317 AAH21989
REF NP_001015859 NP_001069834 NP_001083178 NP_004043 NP_033890
SP O55003 Q12983 Q32KN2
TPG DAA14630