BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7276

Title: Complete 1H, 13C and 15N resonance assignments of Blo t 5, a major mite allergen from Blomia tropicalis.   PubMed: 17206470

Authors: Naik, Mandar; Chang, Chi-Fon; Huang, Tai-Huang

Citation: Naik, Mandar; Chang, Chi-Fon; Kuo, I-Chun; Yu, Tsunai; Fang, Pei-Ju; Chua, Kaw-Yan; Huang, Tai-Huang. "Complete 1H, 13C and 15N resonance assignments of Blo t 5, a major mite allergen from Blomia tropicalis."  J. Biomol. NMR 38, 189-189 (2006).

Assembly members:
Blo t 5, polymer, 119 residues, 14035.8 Da.

Natural source:   Common Name: House dust mite   Taxonomy ID: 40697   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: mites and ticks Blomia tropicalis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Blo t 5: GSQEHKPKKDDFRNEFDHLL IEQANHAIEKGEHQLLYLQH QLDELNENKSKELQEKIIRE LDVVCAMIEGAQGALERELK RTDLNILERFNYEEAQTLSK ILLKDLKETEQKVKDIQTQ

Data sets:
Data typeCount
13C chemical shifts595
15N chemical shifts142
1H chemical shifts903

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Blo t 51

Entities:

Entity 1, Blo t 5 119 residues - 14035.8 Da.

-2 GLY, -1 SER, 1 GLN, 2 GLU, ..., 117 GLN

1   GLYSERGLNGLUHISLYSPROLYSLYSASP
2   ASPPHEARGASNGLUPHEASPHISLEULEU
3   ILEGLUGLNALAASNHISALAILEGLULYS
4   GLYGLUHISGLNLEULEUTYRLEUGLNHIS
5   GLNLEUASPGLULEUASNGLUASNLYSSER
6   LYSGLULEUGLNGLULYSILEILEARGGLU
7   LEUASPVALVALCYSALAMETILEGLUGLY
8   ALAGLNGLYALALEUGLUARGGLULEULYS
9   ARGTHRASPLEUASNILELEUGLUARGPHE
10   ASNTYRGLUGLUALAGLNTHRLEUSERLYS
11   ILELEULEULYSASPLEULYSGLUTHRGLU
12   GLNLYSVALLYSASPILEGLNTHRGLN

Samples:

sample_1: Blo t 5, [U-13C; U-15N], 1 ± 0.05 mM; Potassium Phosphate 50 ± 0.01 mM; Sodium Chloride 100 ± 0.01 mM; EDTA 2 ± 0.01 mM; Sodium Azide 0.001 ± 0.0001 %

sample_2: Blo t 5, [U-15N], 1 ± 0.05 mM; Potassium Phosphate 50 ± 0.01 mM; Sodium Chloride 100 ± 0.01 mM; EDTA 2 ± 0.01 mM; Sodium Azide 0.001 ± 0.0001 %

sample_3: Blo t 5 1 ± 0.05 mM; Potassium Phosphate 50 ± 0.01 mM; Sodium Chloride 100 ± 0.01 mM; EDTA 2 ± 0.01 mM; Sodium Azide 0.001 ± 0.0001 %

sample_4: Blo t 5, [U-10% 13C], 0.8 ± 0.05 mM; Potassium Phosphate 50 ± 0.01 mM; Sodium Chloride 100 ± 0.01 mM; EDTA 2 ± 0.01 mM; Sodium Azide 0.001 ± 0.0001 %

sample_5: Blo t 5, [15N]-Lys,Leu, 0.8 ± 0.05 mM; Potassium Phosphate 50 ± 0.01 mM; Sodium Chloride 100 ± 0.01 mM; EDTA 2 ± 0.01 mM; Sodium Azide 0.001 ± 0.0001 %

ph7.5_295K: pH: 7.5; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCsample_2not availableph7.5_295K
13C HSQCsample_1not availableph7.5_295K
HNCasample_1not availableph7.5_295K
HN(CO)Casample_1not availableph7.5_295K
HNCaCbsample_1not availableph7.5_295K
CbCa(CO)NHsample_1not availableph7.5_295K
HNCOsample_1not availableph7.5_295K
HN(Ca)COsample_1not availableph7.5_295K
HCaCOsample_1not availableph7.5_295K
HbHa(CO)NHsample_1not availableph7.5_295K
(H)C(CO)NHsample_1not availableph7.5_295K
H(CCO)NHsample_1not availableph7.5_295K
15N_TocsyHSQCsample_1not availableph7.5_295K
HCCH-Cosysample_1not availableph7.5_295K
CCH-Tocsysample_1not availableph7.5_295K
HCCH-Tocsysample_1not availableph7.5_295K
Cosysample_3not availableph7.5_295K
15N edited NoesyHSQCnot availablenot availableph7.5_295K
13C edited NoesyHSQCnot availablenot availableph7.5_295K
Tocsysample_3not availableph7.5_295K
Noesysample_3not availableph7.5_295K
(Hb)Cb(CgCd)Hdsample_1not availableph7.5_295K
(Hb)Cb(CgCdCe)Hesample_1not availableph7.5_295K
15N HMBCsample_2not availableph7.5_295K
15N HMQCsample_2not availableph7.5_295K
HNHasample_1not availableph7.5_295K
13C CT-HSQCsample_4not availableph7.5_295K

Software:

xwinnmr v3.5, Bruker - NMR data acquisition, processing

SPARKY v3.112, UCSF - NMR data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 500 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 15345
PDB
GB AAD10850 ABH06351 ABH06352 ABH06353 ABH06354
SP O96870