BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 7259

Title: The solution structure of the BRCT domain from human polymerase reveals homology with the TdT BRCT domain   PubMed: 17915942

Authors: DeRose, E.; Clarkson, M.; Gilmore, S.; Ramsden, D.; Mueller, G.; London, R.; Lee, A.

Citation: DeRose, E.; Clarkson, M.; Gilmore, S.; Galban, C.; Tripathy, A.; Havener, J.; Mueller, G.; Ramsden, D.; London, R.; Lee, A.. "Solution Structure of Polymerase mu's BRCT Domain Reveals an Element Essential for Its Role in Nonhomologous End Joining."  Biochemistry 46, 12100-12110 (2007).

Assembly members:
DNA polymerase mu (E.C.2.7.7.7), polymer, 105 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DNA polymerase mu (E.C.2.7.7.7): GTPPSTRFPGVAIYLVEPRM GRSRRAFLTGLARSKGFRVL DACSSEATHVVMEETSAEEA VSWQERRMAAAPPGCTPPAL LDISWLTESLGAGQPVPVEC RHRLE

Data sets:
Data typeCount
13C chemical shifts413
15N chemical shifts100
1H chemical shifts651

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA polymerase mu1

Entities:

Entity 1, DNA polymerase mu 105 residues - Formula weight is not available

1   GLYTHRPROPROSERTHRARGPHEPROGLY
2   VALALAILETYRLEUVALGLUPROARGMET
3   GLYARGSERARGARGALAPHELEUTHRGLY
4   LEUALAARGSERLYSGLYPHEARGVALLEU
5   ASPALACYSSERSERGLUALATHRHISVAL
6   VALMETGLUGLUTHRSERALAGLUGLUALA
7   VALSERTRPGLNGLUARGARGMETALAALA
8   ALAPROPROGLYCYSTHRPROPROALALEU
9   LEUASPILESERTRPLEUTHRGLUSERLEU
10   GLYALAGLYGLNPROVALPROVALGLUCYS
11   ARGHISARGLEUGLU

Samples:

sample_1: DNA polymerase mu (E.C.2.7.7.7), [U-13C; U-15N], 1 mM; d-Tris 25 mM; KCl 50 mM; NaN3 0.02%; DTT 5 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 50 mM; pH: 7.9; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available

Software:

VNMR v1.1C, VNMRJ 1.1C - collection

NMRPipe v2003, 2004 - processing

NMRView v5.0.4 - processing

CYANA v2.1 - structure solution

ARIA v2.0a - refinement

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAB14353 BAF84327 BAG57686
EMBL CAB65075
GB AAF26284 AAH26306 AAH49202 AAH62590 AAW65376
REF NP_001271259 NP_001271260 NP_037416 XP_001142751 XP_001142835
SP Q9NP87