BMRB Entry 7249

Title:
Complete 1H, 13C, and 15N Chemical Shift Assignments for the 15.5K Protein
Deposition date:
2006-08-02
Original release date:
2006-12-04
Authors:
Soss, Sarah; Flynn, Peter
Citation:

Citation: Soss, Sarah; Flynn, Peter. "NMR assignment of the human spliceosomeal 15.5K protein"  J. Biomol. NMR 38, 175-175 (2007).
PubMed: 17122951

Assembly members:

Assembly members:
His-15.5K, polymer, 144 residues, 16000 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET11a

Data sets:
Data typeCount
13C chemical shifts557
15N chemical shifts130
1H chemical shifts915

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1His-15.5K1

Entities:

Entity 1, His-15.5K 144 residues - 16000 Da.

1   METGLYHISHISHISHISHISHISSERSER
2   GLYILEGLUGLUGLYARGMETTHRGLUALA
3   ASPVALASNPROLYSALATYRPROLEUALA
4   ASPALAHISLEUTHRLYSLYSLEULEUASP
5   LEUVALGLNGLNSERCYSASNTYRLYSGLN
6   LEUARGLYSGLYALAASNGLUALATHRLYS
7   THRLEUASNARGGLYILESERGLUPHEILE
8   VALMETALAALAASPALAGLUPROLEUGLU
9   ILEILELEUHISLEUPROLEULEUCYSGLU
10   ASPLYSASNVALPROTYRVALPHEVALARG
11   SERLYSGLNALALEUGLYARGALACYSGLY
12   VALSERARGPROVALILEALACYSSERVAL
13   THRILELYSGLUGLYSERGLNLEULYSGLN
14   GLNILEGLNSERILEGLNGLNSERILEGLU
15   ARGLEULEUVAL

Samples:

sample_2: His-15.5K, [U-98% 13C; U-98% 15N], 0.9 ± 0.1 mM; sodium phosphate 50 ± 5 mM; sodium chloride 200 ± 20 mM; sodium azide 0.1 ± 0.1 w/w %

sample_1: His-15.5K, [U-98% 13C; U-98% 15N], 1.6 ± 0.2 mM; sodium phosphate 100 ± 10 mM; sodium chloride 100 ± 10 mM; sodium azide 0.1 ± 0.1 w/w %

conditions_2: pH: 6.0; temperature: 297 K

conditions_1: pH: 6.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HNCACBsample_2not availableconditions_2
CBCA_CO_NHsample_2not availableconditions_2
HNCAsample_2not availableconditions_2
HN_CO_CAsample_2not availableconditions_2
HCCH-COSYsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
HBHA_CCO_NHsample_1not availableconditions_1
C_CO_NHsample_1not availableconditions_1
HNCOsample_2not availableconditions_2
1H15N_HSQCsample_1not availableconditions_1
1H13C_HSQCsample_1not availableconditions_1

Software:

FELIX v97.0 - raw data processing

SPARKY - peak picking and assignment

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 600 MHz

Related Database Links:

BMRB 15445
PDB
DBJ BAA23363 BAB23329 BAE01699 BAE22349 BAE26537
EMBL CAG30417 CAG46526 CAK54562 CAK54861
GB AAC72945 AAF06959 AAH05358 AAH19282 AAH26755
PRF 2210268A
REF NP_001003796 NP_001070472 NP_001177155 NP_001177156 NP_001271884
SP P55769 P55770 Q3B8S0 Q4R5C6 Q9D0T1
TPG DAA29093
AlphaFold Q4R5C6 P55769 P55770 Q3B8S0 Q9D0T1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks