BMRB Entry 7216

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for yeast triosephosphate isomerase, TIM
Deposition date:
2006-07-06
Original release date:
2006-08-12
Authors:
Loria, J.; Wang, Chunyu; Massi, Francesca; Rance, Mark; Palmer, Arthur
Citation:

Citation: Loria, J.; Wang, Chunyu; Massi, Francesca; Rance, Mark; Palmer, Arthur. "Backbone 1H, 13C, and 15N Chemical Shift Assignments for yeast triosephosphate isomerase, TIM"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
Triosephosphate isomerase, polymer, 248 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts599
15N chemical shifts180
1H chemical shifts180

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Triosephosphate isomerase, chain 11
2Triosephosphate isomerase, chain 21

Entities:

Entity 1, Triosephosphate isomerase, chain 1 248 residues - Formula weight is not available

1   METALAARGTHRPHEPHEVALGLYGLYASN
2   PHELYSLEUASNGLYSERLYSGLNSERILE
3   LYSGLUILEVALGLUARGLEUASNTHRALA
4   SERILEPROGLUASNVALGLUVALVALILE
5   CYSPROPROALATHRTYRLEUASPTYRSER
6   VALSERLEUVALLYSLYSPROGLNVALTHR
7   VALGLYALAGLNASNALATYRLEULYSALA
8   SERGLYALAPHETHRGLYGLUASNSERVAL
9   ASPGLNILELYSASPVALGLYALALYSTYR
10   VALILELEUGLYHISSERGLUARGARGSER
11   TYRPHEHISGLUASPASPLYSPHEILEALA
12   ASPLYSTHRLYSPHEALALEUGLYGLNGLY
13   VALGLYVALILELEUCYSILEGLYGLUTHR
14   LEUGLUGLULYSLYSALAGLYLYSTHRLEU
15   ASPVALVALGLUARGGLNLEUASNALAVAL
16   LEUGLUGLUVALLYSASPPHETHRASNVAL
17   VALVALALATYRGLUPROVALTRPALAILE
18   GLYTHRGLYLEUALAALATHRPROGLUASP
19   ALAGLNASPILEHISALASERILEARGLYS
20   PHELEUALASERLYSLEUGLYASPLYSALA
21   ALASERGLULEUARGILELEUTYRGLYGLY
22   SERALAASNGLYSERASNALAVALTHRPHE
23   LYSASPLYSALAASPVALASPGLYPHELEU
24   VALGLYGLYALASERLEULYSPROGLUPHE
25   VALASPILEILEASNSERARGASN

Samples:

sample_1: Triosephosphate isomerase, [U-99% 13C; U-99% 15N; U-80% 2H], 0.9 mM; Sodium acetate, [U-2H], 10 mM; DTT 1 mM; Sodium azide 0.02%

conditions_1: pH: 5.9; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N TROSYsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HN(CO)CACBsample_1not availableconditions_1

Software:

SPARKY -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 16565 16566 17122
PDB
DBJ GAA22287
EMBL CAA89080 CAY78558
GB AAA88757 AAS55980 AHY75046 EDN60396 EDV08247
REF NP_010335
SP P00942
TPG DAA11897
AlphaFold P00942

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks