BMRB Entry 7208

Title:
1H, 13C, 15N Chemical shift assignment for Glycophorine A in bicelles and micelles
Deposition date:
2006-07-04
Original release date:
2007-01-09
Authors:
Mineev, Konstantin; Bocharov, Eduard; Maslennikov, Innokenty; Goncharuk, Marina; Arseniev, Alexander
Citation:

Citation: Mineev, Konstantin; Bocharov, Eduard; Maslennikov, Innokenty; Goncharuk, Marina; Arseniev, Alexander. "Structure and dynamics of glycophorin A TM segment in micelles and bicelles"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
GpATM, polymer, 38 residues, 4235 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GpATM: RVQLAHHFSEPEITLIIFGV MAGVIGTILLISYGIRRL

Data sets:
Data typeCount
13C chemical shifts268
15N chemical shifts71
1H chemical shifts567
coupling constants84
T1 relaxation values64
T2 relaxation values64

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1dimer of Glycophorin A transmembrane segments A1
2dimer of Glycophorin A transmembrane segments B1

Entities:

Entity 1, dimer of Glycophorin A transmembrane segments A 38 residues - 4235 Da.

1   ARGVALGLNLEUALAHISHISPHESERGLU
2   PROGLUILETHRLEUILEILEPHEGLYVAL
3   METALAGLYVALILEGLYTHRILELEULEU
4   ILESERTYRGLYILEARGARGLEU

Samples:

bic_hetero: GpATM, [U-15N; U-13C], 1.0 ± 0.2 mM; DMPC, [U-2H], 16.0 ± 1.0 mM; GpATM 1.0 ± 0.2 mM; DHPC, [U-2H], 64.0 ± 1.0 mM

mic_N15: GpATM, [U-15N], 2.0 ± 0.2 mM; Dodecylphosphocholine, [U-2H], 60.0 ± 2.0 mM

mic_N1513C: GpATM, [U-15N; U-13C], 2.0 ± 0.2 mM; Dodecylphosphocholine, [U-2H], 60.0 ± 2.0 mM

bic_15N13C: GpATM, [U-15N; U-13C], 2.0 ± 0.2 mM; DMPC, [U-2H], 16.0 ± 1.0 mM; DHPC, [U-2H], 64.0 ± 1.0 mM

bic_15N: GpATM, [U-15N], 2.0 ± 0.2 mM; DMPC, [U-2H], 16.0 ± 1.0 mM; DHPC, [U-2H], 64.0 ± 1.0 mM

mic_hetero: GpATM, [U-15N; U-13C], 1.0 ± 0.2 mM; Dodecylphosphocholine, [2H], 60.0 ± 2.0 mM; GpATM 1.0 ± 0.2 mM

micelles: pH: 6.0; temperature: 313 K

bicelles: pH: 5.4; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions

Software:

VNMR -

NMR spectrometers:

  • Varian Unity 600 MHz

Related Database Links:

PDB
DBJ BAF83250
EMBL CAA30843 CAA36095 CAC83868 CAC83869 CAC83870
GB AAA18258 AAA52624 AAA52768 AAA88044 AAA88051
PRF 0412211A 1311302A 1314320A 1605205A
REF NP_002090
SP P02724
AlphaFold P02724

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks