BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7205

Title: 1H, 13C, and 15N Chemical Shift Assignments for Transmembrane Segment of ErbB4   PubMed: 18178548

Authors: Mineev, Konstantin; Bocharov, Eduard; Maslennikov, Innokenty; Goncharuk, Marina; Arseniev, Alexander

Citation: Bocharov, Eduard; Mineev, Konstantin; Volynsky, Pavel; Ermolyuk, Y.; Tkach, E.; Sobol, A.; Chupin, V.; Kirpichnikov, M.; Efremov, R.; Arseniev, Alexander. "Spatial structure of the dimeric transmembrane domain of the growth factor receptor ErbB2 presumably corresponding to the receptor active state"  J. Biol. Chem. 283, 6950-6956 (2008).

Assembly members:
ErbB4 TM, polymer, 44 residues, 4760 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo spaiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
ErbB4 TM: STLPQHARTPLIAAGVIGGL FILVIVGLTFAVYVRRKSIK KKRA

Data sets:
Data typeCount
13C chemical shifts179
15N chemical shifts40
1H chemical shifts316
coupling constants37
T1 relaxation values36
T2 relaxation values34

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ErbB4 TM, chain 11
2ErbB4 TM, chain 22

Entities:

Entity 1, ErbB4 TM, chain 1 44 residues - 4760 Da.

1   SERTHRLEUPROGLNHISALAARGTHRPRO
2   LEUILEALAALAGLYVALILEGLYGLYLEU
3   PHEILELEUVALILEVALGLYLEUTHRPHE
4   ALAVALTYRVALARGARGLYSSERILELYS
5   LYSLYSARGALA

Entity 2, ErbB4 TM, chain 2 44 residues - 4760 Da.

1   SERTHRLEUPROGLNHISALAARGTHRPRO
2   LEUILEALAALAGLYVALILEGLYGLYLEU
3   PHEILELEUVALILEVALGLYLEUTHRPHE
4   ALAVALTYRVALARGARGLYSSERILELYS
5   LYSLYSARGALA

Samples:

sample_1: ErbB4 TM, [U-15N], 2.4 ± 0.3 mM; DMPC, [U-2H], 19 ± 1 mM; DHPC, [U-2H], 77 ± 1 mM

sample_2: ErbB4 TM, [U-15N; U-13C], 2.4 ± 0.3 mM; DMPC, [U-2H], 19 ± 1 mM; DHPC, [U-2H], 77 ± 1 mM

sample_3: ErbB4 TM, [U-15N; U-13C], 0.6 ± 0.3 mM; DMPC, [U-2H], 19 ± 1 mM; DHPC, [U-2H], 77 ± 1 mM; ErbB4 TM, none, 0.6 ± 0.3 mM

conditions_1: pH: 5.0; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCnot availablenot availableconditions_1
1H13C HSQCnot availablenot availableconditions_1
HCcH-TOCSYnot availablenot availableconditions_1
3D 13C-ali NOESYnot availablenot availableconditions_1
3D 15N-NOESYnot availablenot availableconditions_1
3D 15N-TOCSYnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
HN(CO)CAnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
HNHAnot availablenot availableconditions_1
HNHBnot availablenot availableconditions_1
2D NC-chirp-NOESYnot availablenot availableconditions_1
3D C-chirp-NOESYnot availablenot availableconditions_1

Software:

VNMR -

NMR spectrometers:

  • Varian Unity 600 MHz

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