BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7192

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Bacillus Subilis YqaI Dimer

Authors: Ramelot, Theresa; Kennedy, Michael

Citation: Ramelot, Theresa; Kennedy, Michael. "NMR structure of YqaI from Bacillus sbutilis"  The BMRB entry is the only known published source for the data..

Assembly members:
YqaI, polymer, 72 residues, Formula weight is not available

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
YqaI: MVENPMVINNWHDKLTETDV QIDFYGDEVTPVDDYVIDGG EIILRENLERYLREQLGFEF KNAQLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts306
15N chemical shifts75
1H chemical shifts482

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YqaI, chain 11
2YqaI, chain 21

Entities:

Entity 1, YqaI, chain 1 72 residues - Formula weight is not available

C-term: LEHHHHHH

1   METVALGLUASNPROMETVALILEASNASN
2   TRPHISASPLYSLEUTHRGLUTHRASPVAL
3   GLNILEASPPHETYRGLYASPGLUVALTHR
4   PROVALASPASPTYRVALILEASPGLYGLY
5   GLUILEILELEUARGGLUASNLEUGLUARG
6   TYRLEUARGGLUGLNLEUGLYPHEGLUPHE
7   LYSASNALAGLNLEUGLUHISHISHISHIS
8   HISHIS

Samples:

sample_1: YqaI, [U-100% 13C; U-100% 15N], 1 mM; MES 20 mM; NaCL 100 mM; DTT 10 mM

conditions_1: ionic strength: 100 mM; pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N NOESYsample_1not availableconditions_1
13C NOESY (aliph)sample_1not availableconditions_1
4D NOESY CC HSQCsample_1not availableconditions_1
15N NOESY in D2Osample_1not availableconditions_1
13C NOESY in D2O (aliph)sample_1not availableconditions_1
13C NOESY (arom)sample_1not availableconditions_1
13C NOESY in D2O (arom)sample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 750 MHz

Related Database Links:

PDB
DBJ BAA06923 BAA12384 BAM58696
EMBL CAB14571 CEI57855 CEJ78277
GB ADV93390 AGG62031 AHA78516 AIC41086 AIC45318
REF NP_390507 WP_003229905 WP_021480131 YP_004204417 YP_007534614
SP P45906