BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7191

Title: 1H, 13C, and 15N Chemical Shift Assignments for Pseudomonas aeruginosa Hypothetical Protein RPA1041: Northeast Structural Genomics Consortium target Pat90

Authors: Eletsky, Alexander; Atreya, Hanudatta; Liu, Gaohua; Sukumaran, Dinesh; Garcia, Maite; Yee, Adelinda; Arrowsmith, Cheryl; Szyperski, Thomas

Citation: Eletsky, Alexander; Atreya, Hanudatta; Liu, Gaohua; Sukumaran, Dinesh; Garcia, Maite; Yee, Adelinda; Arrowsmith, Cheryl; Szyperski, Thomas. "Solution NMR Structure of Pseudomonas aeruginosa Hypothetical Protein RPA1041"  Proteins: Struct. Funct. Genet. ., .-..

Assembly members:
pat90, polymer, 97 residues, Formula weight is not available

Natural source:   Common Name: P. aeruginosa   Taxonomy ID: 287   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
pat90: MGSSHHHHHHSSGRENLYFQ GHLRELLRTNDAVLLSAVGA LLDGADIGHLVLDQNMSILE GSLGVIPRRVLVHEDDLAGA RRLLTDAGLAHELRSDD

Data sets:
Data typeCount
13C chemical shifts337
15N chemical shifts86
1H chemical shifts570

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pat90 monomer1

Entities:

Entity 1, pat90 monomer 97 residues - Formula weight is not available

Contains 22-residue N-terminal His-tag MGSSHHHHHHSSGRENLYFQGH

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYARGGLUASNLEUTYRPHEGLN
3   GLYHISLEUARGGLULEULEUARGTHRASN
4   ASPALAVALLEULEUSERALAVALGLYALA
5   LEULEUASPGLYALAASPILEGLYHISLEU
6   VALLEUASPGLNASNMETSERILELEUGLU
7   GLYSERLEUGLYVALILEPROARGARGVAL
8   LEUVALHISGLUASPASPLEUALAGLYALA
9   ARGARGLEULEUTHRASPALAGLYLEUALA
10   HISGLULEUARGSERASPASP

Samples:

NC: pat90, [U-100% 13C; U-100% 15N], 0.5 mM; MOPS 10 mM; NaCl 450 mM; NaN3 0.01 % w/w; ZnSO4 10 mM; DTT 10 mM; benzamidine 1 mM; inhibitor cocktail 1

NC7: pat90, [U-7% 13C; U-100% 15N], 0.5 mM; MOPS 10 mM; NaCl 450 mM; NaN3 0.01 % w/w; ZnSO4 10 mM; DTT 10 mM; benzamidine 1 mM; inhibitor cocktail 1

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H15N_HSQCNCisotropicconditions_1
2D 1H13C_HSQC (ali)NCisotropicconditions_1
2D 1H13C_HSQC (aro)NCisotropicconditions_1
2D CT-1H13C_HSQC (ali)NCisotropicconditions_1
2D CT-1H13C_HSQC (aro)not availablenot availableconditions_1
3D HNCONCisotropicconditions_1
(4,3)D GFT CABCA(CO)NHNNCisotropicconditions_1
3D HAB(CO)NHNCisotropicconditions_1
(4,3)D GFT HNNCABCANCisotropicconditions_1
(4,3)D GFT HCCH-COSY (ali)NCisotropicconditions_1
3D (H)CCH-COSY (ali)NCisotropicconditions_1
3D 15N-, 13Cali-, 13Caro-resolved NOESYNCisotropicconditions_1
2D 28ms CT-1H13C_HSQC (methyl)not availablenot availableconditions_1
2D 56ms CT-1H13C_HSQC (methyl)not availablenot availableconditions_1
3D (H)CCH-TOCSY (ali)NCisotropicconditions_1

Software:

VNMR v6.1C, Varian, Inc. - NMR data acqusition

XEASY v1.3.13, Bartels et al. - Spectral analysis

CARA v1.5.3, Keller and Wuthrich - Spectral analysis

PROSA v6.0, Guntert - NMR data processing

NMR spectrometers:

  • Varian, Inc INOVA 600 MHz
  • Varian, Inc INOVA 750 MHz

Related Database Links:

PDB
EMBL CAE26484
GB ACE99772
REF NP_946392 WP_011156574 WP_012494775 YP_001990248