BMRB Entry 7190

Title:
The structure and function of a novel two-site calcium-binding fragment of calmodulin
Deposition date:
2006-06-22
Original release date:
2007-09-24
Authors:
Lakowski, Ted; Lee, Greg; Reid, Ronald; McIntosh, Lawrence
Citation:

Citation: Lakowski, Ted; Lee, Greg; Okon, M.; Reid, Ronald; McIntosh, Lawrence. "Calcium-induced folding of a fragment of calmodulin composed of EF-hands 2 and 3"  Protein Sci. 16, 1119-1132 (2007).
PubMed: 17473011

Assembly members:

Assembly members:
CaM2/3, polymer, 68 residues, 7701 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET28a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts283
15N chemical shifts70
1H chemical shifts449
heteronuclear NOE values64
T1 relaxation values64
T2 relaxation values64

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CaM2/31
2CALCIUM (II) ION, 12
3CALCIUM (II) ION, 22

Entities:

Entity 1, CaM2/3 68 residues - 7701 Da.

1   ALAGLULEUGLNASPMETILEASNGLUVAL
2   ASPALAASPGLYASNGLYTHRILEASPPHE
3   PROGLUPHELEUTHRMETMETALAARGLYS
4   METLYSASPTHRASPSERGLUGLUGLUILE
5   ARGGLUALAPHEARGVALPHEASPLYSASP
6   GLYASNGLYTYRILESERALAALAGLULEU
7   ARGHISVALMETTHRASNLEUGLY

Entity 2, CALCIUM (II) ION, 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: CaM2/3, [U-13C; U-15N], 0.5 mM; Tris d11, 11 D, 20 mM; CaCl2 10 mM; KCl 50 mM

sample_2: CaM2/3, [U-15N], 0.2 mM; Tris d11, 11 D, 20 mM; CaCl2 10 mM; KCl 50 mM

conditions_1: pH*: 7.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N-1H NOESY-HSQCnot availablenot availableconditions_1
SIMULTANEOUS 1H-13C/15N-1Hnot availablenot availableconditions_1
NOESY-HSQCnot availablenot availableconditions_1
AROMATIC 1H-13C-1Hnot availablenot availableconditions_1
NOESY-HSQCnot availablenot availableconditions_1
METHYL 1H-13C/15N-1H NOESY-HSQCnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Unity 500 MHz
  • Varian Inova 600 MHz

Related Database Links:

BMRB 11440 16994 19376 25344 6789 7016 7017
PDB
DBJ BAC39089 BAF45809 BAI66109 BAI66110 BAI66111
EMBL CAB51566 CAB65357 CCJ31183
GB AAB87698 AAC23998 AAC23999 AAH07965 AAH10730
REF XP_001687795 XP_001869425 XP_002404770 XP_004322571 XP_004610315
TPG DAA24989 DAA24990

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks