BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 7147

Title: The acute myeloid leukemia fusion protein AML1-ETO targets E-proteins via a PAH-like TAFH domain   PubMed: 16803958

Authors: Plevin, M.; Zhang, J.; Guo, C.; Roeder, R.; Ikura, M.

Citation: Plevin, M.; Zhang, J.; Guo, C.; Roeder, R.; Ikura, M.. "The acute myeloid leukemia fusion protein AML1-ETO targets E proteins via a paired amphipathic helix-like TBP-associated factor homology domain"  Proc. Natl. Acad. Sci. U.S.A. 103, 10242-10247 (2006).

Assembly members:
Core-binding factor, ML1-ETO, polymer, 105 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Core-binding factor, ML1-ETO: GSARQLSKLKRFLTTLQQFG NDISPEIGERVRTLVLGLVN STLTIEEFHSKLQEATNFPL RPFVIPFLKANLPLLQRELL HAARLAKQNPAQYLAQHEQL LLDAS

Data sets:
Data typeCount
13C chemical shifts326
15N chemical shifts96
1H chemical shifts768

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Core-binding factor, ML1-ETO1

Entities:

Entity 1, Core-binding factor, ML1-ETO 105 residues - Formula weight is not available

1   GLYSERALAARGGLNLEUSERLYSLEULYS
2   ARGPHELEUTHRTHRLEUGLNGLNPHEGLY
3   ASNASPILESERPROGLUILEGLYGLUARG
4   VALARGTHRLEUVALLEUGLYLEUVALASN
5   SERTHRLEUTHRILEGLUGLUPHEHISSER
6   LYSLEUGLNGLUALATHRASNPHEPROLEU
7   ARGPROPHEVALILEPROPHELEULYSALA
8   ASNLEUPROLEULEUGLNARGGLULEULEU
9   HISALAALAARGLEUALALYSGLNASNPRO
10   ALAGLNTYRLEUALAGLNHISGLUGLNLEU
11   LEULEUASPALASER

Samples:

sample_1: Core-binding factor, ML1-ETO, [U-15N; U-13C], 0.7 mM; sodium phosphate 20 mM; NaCl 50 mM; PMSF 1 mM; AEBSF 1 mM; sodium azide 0.25 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 70 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1not availablesample_cond_1
4D 13C/15N-separated NOESYsample_1not availablesample_cond_1

Software:

CYANA - refinement, structure solution

NMR spectrometers:

  • Varian UNITYplus 800 MHz

Related Database Links:

BMRB 16467 7396
PDB
DBJ BAA03089 BAA03247 BAA03558 BAA03559 BAA03560
EMBL CAA56311 CAF99400 CAG33073
GB AAB34819 AAB34820 AAC28931 AAC28932 AAC28933
REF NP_001070244 NP_001089065 NP_001101923 NP_001102127 NP_001104496
SP O75081 Q06455 Q61909