BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7127

Title: Fibronectin 2F3 chemical shift assignments   PubMed: 17464288

Authors: Vakonakis, Ioannis; Campbell, Iain

Citation: Vakonakis, Ioannis; Staunton, David; Rooney, Luke; Campbell, Iain. "Interdomain association in fibronectin: insight into cryptic sites and fibrillogenesis."  EMBO J. 26, 2575-2583 (2007).

Assembly members:
2F3, polymer, 95 residues, 9833.7 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
2F3: GPLGSHLVATSESVTEITAS SFVVSWVSASDTVSGFRVEY ELSEEGDEPQYLDLPSTATS VNIPDLLPGRKYIVNVYQIS EDGEQSLILSTSQTT

Data sets:
Data typeCount
13C chemical shifts318
15N chemical shifts97
1H chemical shifts645

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
12F31

Entities:

Entity 1, 2F3 95 residues - 9833.7 Da.

The 2F3 sequence corresponds to that of the second type III domain of fibronectin with an additional 6 non-canonical amino-terminal residues from the expression system

1   GLYPROLEUGLYSERHISLEUVALALATHR
2   SERGLUSERVALTHRGLUILETHRALASER
3   SERPHEVALVALSERTRPVALSERALASER
4   ASPTHRVALSERGLYPHEARGVALGLUTYR
5   GLULEUSERGLUGLUGLYASPGLUPROGLN
6   TYRLEUASPLEUPROSERTHRALATHRSER
7   VALASNILEPROASPLEULEUPROGLYARG
8   LYSTYRILEVALASNVALTYRGLNILESER
9   GLUASPGLYGLUGLNSERLEUILELEUSER
10   THRSERGLNTHRTHR

Samples:

sample_1: 2F3, [U-15N], 2 ± 0.2 mM; NaCl 20 mM; NaPi 20 mM; D2O 5%; H2O 95%

sample_2: 2F3, [U-13C; U-15N], 1.5 ± 0.1 mM; NaCl 20 mM; NaPi 20 mM; D2O 5%; H2O 95%

conditions_1: ionic strength: 160 mM; pH: 7.0; pressure: 1 atm; temperature: 303 K

conditions_2: ionic strength: 160 mM; pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCsample_1not availableconditions_1
CBCA(CO)NHsample_2not availableconditions_1
CBCANHsample_2not availableconditions_1
C(CO)NHsample_2not availableconditions_1
H(CCO)NHsample_2not availableconditions_1
HC(C)H-COSYsample_2not availableconditions_2
HCHC 4D NOESYsample_2not availableconditions_2
HBHA(CO)NHsample_2not availableconditions_1
HNHAsample_1not availableconditions_1

Software:

Omega Spectrometer Operating Software vBeta 6.0.3b2, Bruker - Data collection

NMRPipe v2.3 Rev 2005.319.11.22, NIH - Data processing

PIPP v4.3.7, NIH - Data analysis

NMR spectrometers:

  • home built . 600 MHz
  • home built . 500 MHz

Related Database Links:

BMRB 7128
PDB