BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7124

Title: Solution Structure of MID1 B-box2 domain: A defining domain in TRIM/RBCC proteins reveals possible versatility in zinc-coordination   PubMed: 16529770

Authors: Matts, Jessica; Short, Kieran; Simmons, Brandi; Singireddy, Suryaparkash; Zou, Julie; Cox, Timothy; Massiah, Michael

Citation: Massiah, Michael; Simmons, Brandi; Short, Kieran; Cox, Timothy. "Solution structure of the RBCC/TRIM B-box1 domain of human MID1: B-box with a RING."  J. Mol. Biol. 358, 532-545 (2006).

Assembly members:
B-box2, polymer, 47 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
B-box2: SHIRGLMCLEHEDEKVNMYC VTDDQLICALCKLVGRHRDH QVAALSE

Data sets:
Data typeCount
13C chemical shifts124
15N chemical shifts47
1H chemical shifts282

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1B-box2 monomer1
2ZINC (II) ION, 12

Entities:

Entity 1, B-box2 monomer 47 residues - Formula weight is not available

1   SERHISILEARGGLYLEUMETCYSLEUGLU
2   HISGLUASPGLULYSVALASNMETTYRCYS
3   VALTHRASPASPGLNLEUILECYSALALEU
4   CYSLYSLEUVALGLYARGHISARGASPHIS
5   GLNVALALAALALEUSERGLU

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

Sample_1: B-box2 0.75 mM; ZN mM; H2O 90%; D2O 10%

conditions_1: ionic strength: 100 mM; pH: 7.5; pressure: 1 atm; temperature: 294 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCSample_1not availableconditions_1
HNCASample_1not availableconditions_1
HNCACBSample_1not availableconditions_1
15N/15N_HSQC-NOESY-HSQCSample_1not availableconditions_1
1H15N_NOESY-HSQCSample_1not availableconditions_1
1H13C_NOESY-HSQCSample_1not availableconditions_1
1H13C_HCCH-TOCSYSample_1not availableconditions_1

Software:

NMRPipe vn/a, NIH - data processing

SPARKY v3.110, UCSF - data analysis

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

PDB
DBJ BAE22636 BAF83904 BAG37559
EMBL CAA74018 CAA75113
GB AAB83986 AAB99951 AAC32998 AAC32999 AAC33000
REF NP_000372 NP_001092094 NP_001179751 NP_001180206 NP_001180207
SP O15344 O70583 P82457 P82458
TPG DAA12559 DAA12560