BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7112

Title: 1H, 13C, and 15N NMR Assignment of the Master-Rep Protein Nuclease Domain (2-95) from the Faba Bean Necrotic Yellows Virus   PubMed: 17043745

Authors: Vega-Rocha, Susana; Campos-Olivas, Ramon

Citation: Vega-Rocha, Susana; Gronenborn, A.; Gronenborn, B.; Campos-Olivas, R.. "1H, 13C, and 15N NMR assignment of the master Rep protein nuclease domain from the Nanovirus FBNYV"  J. Biomol. NMR 38, 169-169 (2007).

Assembly members:
FBNYV_M-Rep_(2-95), polymer, 94 residues, 10825.3 Da.

Natural source:   Common Name: Faba Bean Necrotic Yellows Virus   Taxonomy ID: 59817   Superkingdom: Viruses   Kingdom: not available   Genus/species: Nanovirus Faba Bean Necrotic Yellows Virus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
FBNYV_M-Rep_(2-95): ARQVICWCFTLNNPLSPLSL HDSMKYLVYQTEQGEAGNIH FQGYIEMKKRTSLAGMKKLI PGAHFEKRRGTQGEARAYSM KEDTRLEGPWEYGE

Data sets:
Data typeCount
13C chemical shifts415
15N chemical shifts99
1H chemical shifts665
coupling constants70
heteronuclear NOE values59

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FBNYV_M-Rep_(2-95)1

Entities:

Entity 1, FBNYV_M-Rep_(2-95) 94 residues - 10825.3 Da.

1   ALAARGGLNVALILECYSTRPCYSPHETHR
2   LEUASNASNPROLEUSERPROLEUSERLEU
3   HISASPSERMETLYSTYRLEUVALTYRGLN
4   THRGLUGLNGLYGLUALAGLYASNILEHIS
5   PHEGLNGLYTYRILEGLUMETLYSLYSARG
6   THRSERLEUALAGLYMETLYSLYSLEUILE
7   PROGLYALAHISPHEGLULYSARGARGGLY
8   THRGLNGLYGLUALAARGALATYRSERMET
9   LYSGLUASPTHRARGLEUGLUGLYPROTRP
10   GLUTYRGLYGLU

Samples:

sample_1: (2-95) M-Rep FBNYV, [U-10% 13C; U-15N], 0.5 mM; Sodium phosphate 20 mM; NaCl 600 mM; DTT 1 mM; D2O 8%

sample_2: (2-95) M-Rep FBNYV, [U-13C; U-15N], 0.5 mM; Sodium phosphate 20 mM; NaCl 600 mM; DTT 1 mM; D2O 8%

sample_3: 2-95 Rep FBNYV, [U-13C; U-15N], 0.5 mM; Sodium phosphate 20 mM; NaCl 600 mM; DTT 1 mM; D2O 100%

conditions_1: ionic strength: 1.264 M; pH: 6.6; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H15N_HSQCnot availablenot availableconditions_1
2D 1H15N_HSQC long rangenot availablenot availableconditions_1
2D 1H13C_HSQC/HMQCnot availablenot availableconditions_1
2D HNCOCG aromnot availablenot availableconditions_1
2D HNCO aromnot availablenot availableconditions_1
3D HNCOnot availablenot availableconditions_1
3D HNCACBnot availablenot availableconditions_1
3D CBCA (CO) NHnot availablenot availableconditions_1
3D HNHAnot availablenot availableconditions_1
3D HNHBnot availablenot availableconditions_1
3D 15N edited TOCSYnot availablenot availableconditions_1
3D 15N edited NOESYnot availablenot availableconditions_1
3D HCACOnot availablenot availableconditions_1
3D CC (CO) NHnot availablenot availableconditions_1
HCCH TOCSYnot availablenot availableconditions_1
4D 15N, 13C NOESYnot availablenot availableconditions_1
4D 13C, 13C NOESYnot availablenot availableconditions_1

Software:

NMRView v5.0.20 -

NMRPipe v97.027.12.56 -

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz

Related Database Links:

BMRB 7218
PDB
EMBL CAA72209 CAB44020
GB ACX50503 AHC72237 AHC72255 AHC72263
REF NP_619567
SP O39828 Q9WIJ5