BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 7036

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for 3rd SH3 domain of human NCK2 adaptor protein   PubMed: 16752908

Authors: Liu, Jingxian

Citation: Liu, Jingxian; Li, Minfen; Ran, Xiaoyuan; Fan, Jing-song; Song, Jianxing. "Structural Insight into the Binding Diversity between the Human Nck2 SH3 Domains and Proline-Rich Proteins(,)"  Biochemistry 45, 7171-7184 (2006).

Assembly members:
NCK2 3rd SH3, polymer, 61 residues, 6822 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
NCK2 3rd SH3: GSHVVQTLYPFSSVTEEELN FEKGETMEVIEKPENDPEWW KCKNARGQVGLVPKNYVVVL S

Data sets:
Data typeCount
13C chemical shifts177
15N chemical shifts55
1H chemical shifts338
T1 relaxation values54
T2 relaxation values54

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NCK2 3rd SH31

Entities:

Entity 1, NCK2 3rd SH3 61 residues - 6822 Da.

1   GLYSERHISVALVALGLNTHRLEUTYRPRO
2   PHESERSERVALTHRGLUGLUGLULEUASN
3   PHEGLULYSGLYGLUTHRMETGLUVALILE
4   GLULYSPROGLUASNASPPROGLUTRPTRP
5   LYSCYSLYSASNALAARGGLYGLNVALGLY
6   LEUVALPROLYSASNTYRVALVALVALLEU
7   SER

Samples:

sample_1: NCK2 3rd SH3, [U-95% 15N], 2.0 mM; phosphate 20 mM; DTT 10 mM; D2O 10%

sample_2: NCK2 3rd SH3, [U-95% 13C; U-95% 15N], 2.0 mM; phosphate 20 mM; DTT 10 mM; D2O 10%

sample_3: NCK2 3rd SH3, [U-95% 13C; U-95% 15N], 2.0 mM; phosphate 20 mM; DTT 10 mM; D2O 70%

conditions_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availablenot available
HSQC TOCSYsample_1not availablenot available
HSQC NOESYsample_1not availablenot available
HNCACB/CBCANHsample_2not availablenot available
HCCH TOCSY/HCCH NOESYsample_3not availablenot available
T1/T2/HetNOEsample_1not availablenot available

Software:

NMRPipe - raw data processing

NMRView - peak assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAC04831 AAY24332 EPQ18601
PIR B46243
REF XP_006103064