BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 7035

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for 2nd SH3 domain of human NCK2 adaptor protein   PubMed: 16752908

Authors: Liu, Jingxian

Citation: Liu, Jingxian; Li, Minfen; Ran, Xiaoyuan; Fan, Jing-song; Song, Jianxing. "Structural Insight into the Binding Diversity between the Human Nck2 SH3 Domains and Proline-Rich Proteins"  Biochemistry 45, 7171-7184 (2006).

Assembly members:
NCK2 2nd SH3, polymer, 57 residues, 6559 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
NCK2 2nd SH3: IPAFVKFAYVAEREDELSLV KGSRVTVMEKCSDGWWRGSY NGQIGWFPSNYVLEEVD

Data sets:
Data typeCount
13C chemical shifts99
15N chemical shifts53
1H chemical shifts311
T1 relaxation values63
T2 relaxation values63

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NCK2 2nd SH31

Entities:

Entity 1, NCK2 2nd SH3 57 residues - 6559 Da.

1   ILEPROALAPHEVALLYSPHEALATYRVAL
2   ALAGLUARGGLUASPGLULEUSERLEUVAL
3   LYSGLYSERARGVALTHRVALMETGLULYS
4   CYSSERASPGLYTRPTRPARGGLYSERTYR
5   ASNGLYGLNILEGLYTRPPHEPROSERASN
6   TYRVALLEUGLUGLUVALASP

Samples:

sample_1: NCK2 2nd SH3, [U-95% 15N], 1.4 ± 0.1 mM; phosphate 20 ± 2 mM; DTT 10 ± 2 mM; D2O 10 ± 2 %

sample_2: NCK2 2nd SH3, [U-95% 13C; U-95% 15N], 1.4 ± 0.1 mM; phosphate 20 ± 2 mM; DTT 10 ± 2 mM; D2O 70 ± 2 %

sample_3: NCK2 2nd SH3, [U-95% 13C; U-95% 15N], 1.4 ± 0.1 mM; phosphate 20 ± 2 mM; DTT 10 ± 2 mM; D2O 10 ± 2 %

conditions_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCnot availablenot availableconditions_1
HSQC-TOCSYsample_1not availableconditions_1
HSQC-NOESYsample_1not availableconditions_1
HNCACB/CBCANHsample_3not availableconditions_1
HCCH TOCSY/HCCH NOESYnot availablenot availableconditions_1
T1/T2/HetNOEnot availablenot availableconditions_1

Software:

NMRPipe - raw data processing

NMRView - peak assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAI47129
GB AAC04831 AAC80284 AAH00103 AAH07195 AAY24332
REF NP_001004720 NP_003572 XP_001109452 XP_001109500 XP_001488099
SP O43639