BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7033

Title: GSPT1/eRF3a(PAM2-2)-PABC complex

Authors: Osawa, Masanori; Shimada, Ichio

Citation: Osawa, Masanori; Shimada, Ichio. "1H Assigned Chemical Shifts for eRF3a, and 1H, 13C, and 15N Assigned Chemical Shifts for PABC"  J. Biol. Chem. ., .-..

Assembly members:
PAM2-2, polymer, 22 residues, Formula weight is not available
PABC domain, polymer, 83 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: not available   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculas

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
PAM2-2: AKPFVPNVHAAEFVPSFLRG PA
PABC domain: GQEPLTASMLASAPPQEQKQ MLGERLFPLIQAMHPTLAGK ITGMLLEIDNSELLHMLESP ESLRSKVDEAVAVLQAHQAK EAA

Data sets:
Data typeCount
13C chemical shifts249
15N chemical shifts81
1H chemical shifts729

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PAM2-21
2PABC2

Entities:

Entity 1, PAM2-2 22 residues - Formula weight is not available

1   ALALYSPROPHEVALPROASNVALHISALA
2   ALAGLUPHEVALPROSERPHELEUARGGLY
3   PROALA

Entity 2, PABC 83 residues - Formula weight is not available

1   GLYGLNGLUPROLEUTHRALASERMETLEU
2   ALASERALAPROPROGLNGLUGLNLYSGLN
3   METLEUGLYGLUARGLEUPHEPROLEUILE
4   GLNALAMETHISPROTHRLEUALAGLYLYS
5   ILETHRGLYMETLEULEUGLUILEASPASN
6   SERGLULEULEUHISMETLEUGLUSERPRO
7   GLUSERLEUARGSERLYSVALASPGLUALA
8   VALALAVALLEUGLNALAHISGLNALALYS
9   GLUALAALA

Samples:

sample_1: PAM2-1, unlabeled, 1 mM; PABC domain, [U-13C; U-15N], 1 mM; NaCl 150 mM

conditions_1: ionic strength: 0.15 M; pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
15N-1H HSQCsample_1not availableconditions_1
13C-1H HSQCsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
C(CO)NHsample_1not availableconditions_1
H(CCO)NHsample_1not availableconditions_1
HBHA(CBCACO)NHsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
15N-edited TOCSY HSQCsample_1not availableconditions_1
15N-edited NOESY HSQCsample_1not availableconditions_1
13C,15N-filterd NOESYsample_1not availableconditions_1
13C,15N-filterd TOCSYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAA91612 BAA92160 BAE22025 BAJ20641 BAC32110 BAC40951 BAC56450 BAE21553 BAE30919
EMBL CAB91089 CAG31442 CAA40721 CAA46522 CAA68428 CAA88401 CAB96752
GB AAH09503 AAH31640 AAH36077 AAH79092 AAI34652 AAA60936 AAD08718 AAH03870 AAH04587 AAH11207
REF NP_001003978 NP_001091540 NP_001123478 NP_001123480 NP_001129149 NP_001005051 NP_001026768 NP_001080204 NP_001085069 NP_001126097
SP Q8IYD1 Q8R050 P11940 P20965 P29341 P61286 Q5R8F7
TPG DAA15601 DAA12894 DAA22579
BMRB 10019 4915