BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7022

Title: Letter to the Editor: 1H, 13C and 15N backbone resonance assignments of DUF589 domain from human HSPC144 protein   PubMed: 16865415

Deposition date: 2006-03-15 Original release date: 2006-08-07

Authors: Shi, Yanhong; Song, Aixin; Chang, Yonggang; Hu, Hongyu; Lin, Donghai

Citation: Shi, Yan-hong; Song, Ai-xin; Chang, Yong-gang; Hu, Hong-yu; Lin, Dong-hai. "1H, 13C and 15N backbone resonance assignments of the DUF589 domain from human HSPC144 protein."  J. Biomol. NMR 36, 57-57 (2006).

Assembly members:
DUF589, polymer, 176 residues, 20734 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
DUF589: MSHWLMKSEPESRLEKGVDV KFSIEDLKAQPKQTTCWDGV RNYQARNFLRAMKLGEEAFF YHSNCKEPGIAGLMKIVKEA YPDHTQFEKNNPHYDPSSKE DNPKWSMVDVQFVRMMKRFI PLAELKSYHQAHKATGGPLK NMVLFTRQRLSIQPLTQEEF DFVLSLEELEHHHHHH

Data sets:
Data typeCount
13C chemical shifts606
15N chemical shifts151
1H chemical shifts871

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DUF589 monomer1

Entities:

Entity 1, DUF589 monomer 176 residues - 20734 Da.

1   METSERHISTRPLEUMETLYSSERGLUPRO
2   GLUSERARGLEUGLULYSGLYVALASPVAL
3   LYSPHESERILEGLUASPLEULYSALAGLN
4   PROLYSGLNTHRTHRCYSTRPASPGLYVAL
5   ARGASNTYRGLNALAARGASNPHELEUARG
6   ALAMETLYSLEUGLYGLUGLUALAPHEPHE
7   TYRHISSERASNCYSLYSGLUPROGLYILE
8   ALAGLYLEUMETLYSILEVALLYSGLUALA
9   TYRPROASPHISTHRGLNPHEGLULYSASN
10   ASNPROHISTYRASPPROSERSERLYSGLU
11   ASPASNPROLYSTRPSERMETVALASPVAL
12   GLNPHEVALARGMETMETLYSARGPHEILE
13   PROLEUALAGLULEULYSSERTYRHISGLN
14   ALAHISLYSALATHRGLYGLYPROLEULYS
15   ASNMETVALLEUPHETHRARGGLNARGLEU
16   SERILEGLNPROLEUTHRGLNGLUGLUPHE
17   ASPPHEVALLEUSERLEUGLUGLULEUGLU
18   HISHISHISHISHISHIS

Samples:

sample_1: DUF589 1 mM; Na2HPO4 15 mM; NaH2PO4 15 mM; NaCl 100 mM; DTT 5 mM; NaN3 0.05%

sample_2: DUF589, [U-13C; U-15N], 1 mM; Na2HPO4 15 mM; NaH2PO4 15 mM; NaCl 100 mM; DTT 5 mM; NaN3 0.05%; H20 100%

sample_3: DUF589, [U-13C; U-15N], 1 mM; Na2HPO4 15 mM; NaH2PO4 15 mM; NaCl 100 mM; DTT 5 mM; NaN3 0.05%; D20 100%

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N-HSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(C0)NHsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HBHA(CO)NHsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
HCCH-COSYsample_1not availableconditions_1

Software:

SPARKY 3, T. D. Goddard and D. G. Kneller -

NMR spectrometers:

  • Varian UNITY INOVA 600 MHz

Related Database Links:

PDB
GB AAF29108 AAG14949 AAG43118 AAH06978 AAH93074
REF NP_001032381 NP_001032382 NP_054893 NP_954994 NP_954995
SP Q9P016

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts