BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6999

Title: KorA   PubMed: 16944275

Authors: Rajasekar, Karthik; Bingle, Lewis; Thomas, Christopher; Hyde, Eva

Citation: Rajasekar, Karthik; Bingle, Lewis; Thomas, Christopher; Hyde, Eva. "1H, 13C and 15N assignments of the KorA global transcriptional repressor protein from the low copy number IncP-1 plasmid, RK2"  J. Biomol. NMR 36, 71-71 (2006).

Assembly members:
KorA, polymer, 101 residues, 11303 Da.

Natural source:   Common Name: Klebsiella aerogenes   Taxonomy ID: 28451   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Klebsiella aerogenes

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
KorA: MKKRLTESQFQEAIQGLEVG QQTIEIARGVLVDGKPQATF ATSLGLTRGAVSQAVHRVWA AFEDKNLPEGYARVTAVLPE HQAYIVRKWEADAKKKQETK R

Data sets:
Data typeCount
13C chemical shifts419
15N chemical shifts107
1H chemical shifts587

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KorA monomer, chain 11
2KorA monomer, chain 21

Entities:

Entity 1, KorA monomer, chain 1 101 residues - 11303 Da.

1   METLYSLYSARGLEUTHRGLUSERGLNPHE
2   GLNGLUALAILEGLNGLYLEUGLUVALGLY
3   GLNGLNTHRILEGLUILEALAARGGLYVAL
4   LEUVALASPGLYLYSPROGLNALATHRPHE
5   ALATHRSERLEUGLYLEUTHRARGGLYALA
6   VALSERGLNALAVALHISARGVALTRPALA
7   ALAPHEGLUASPLYSASNLEUPROGLUGLY
8   TYRALAARGVALTHRALAVALLEUPROGLU
9   HISGLNALATYRILEVALARGLYSTRPGLU
10   ALAASPALALYSLYSLYSGLNGLUTHRLYS
11   ARG

Samples:

sample_1: KorA, [U-13C; U-15N], 1 mM; Phosphate Buffer 25 mM; EDTA 0.1 mM

conditions_1: pH: 6.2; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HB(CB)HA(CA)CONHsample_1not availableconditions_1
HB(CB)HA(CA)NHsample_1not availableconditions_1
HC(C)H-TOCSYsample_1not availableconditions_1
TOCSY-15N HSQCsample_1not availableconditions_1
(H)CC(CO)NH-TOCSYsample_1not availableconditions_1
H(CC)(CO)NH-TOCSYsample_1not availableconditions_1
2D 1H-1H TOCSYsample_1not availableconditions_1
(HB) CB (CG CD) HDsample_1not availableconditions_1
(HB) CB (CG CD CE) HEsample_1not availableconditions_1

Software:

CCPN v1.0.8 -

NMRPipe -

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian Unity Plus 600 MHz

Related Database Links:

PDB
DBJ BAI47895 BAI47938 BAJ06625
EMBL CAA27596 CAG30914 CAK12746
GB AAK73380 AAO47408 AAO47415 AAO47422 AAO47428
PIR RGECKK
REF WP_011205830 YP_006941392 YP_006941474 YP_006941560 YP_009065236
SP P03052
TPE CAJ85740