BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6988

Title: 1H, 13C, and 15N Chemical Shift Assignments for HGB1-UBA   PubMed: 16731964

Authors: Chang, Y.G.; Song, A.X.; Gao, Y.G.; Shi, Y.H.; Lin, X.J.; Cao, X.T.; Lin, D.H.; Hu, H.Y.

Citation: Chang, Y.G.; Song, A.X.; Gao, Y.G.; Shi, Y.H.; Lin, X.J.; Cao, X.T.; Lin, D.H.; Hu, H.Y.. "Solution structure of the ubiquitin-associated domain of human BMSC-UbP and its complex with ubiquitin"  Protein Sci. 15, 1248-1259 (2006).

Assembly members:
HGB1-UBA, polymer, 108 residues, 12000 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
HGB1-UBA: MHHHHHHQYKLALNGKTLKG ETTTEAVDAATAEKVFKQYA NDNGVDGEWTYDDATKTFTV TEGSQWQPQLQQLRDMGIQD DELSLRALQATGGDIQAALE LIFAGGAP

Data sets:
Data typeCount
13C chemical shifts397
15N chemical shifts103
1H chemical shifts639

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HGB1-UBA monomer1

Entities:

Entity 1, HGB1-UBA monomer 108 residues - 12000 Da.

1   METHISHISHISHISHISHISGLNTYRLYS
2   LEUALALEUASNGLYLYSTHRLEULYSGLY
3   GLUTHRTHRTHRGLUALAVALASPALAALA
4   THRALAGLULYSVALPHELYSGLNTYRALA
5   ASNASPASNGLYVALASPGLYGLUTRPTHR
6   TYRASPASPALATHRLYSTHRPHETHRVAL
7   THRGLUGLYSERGLNTRPGLNPROGLNLEU
8   GLNGLNLEUARGASPMETGLYILEGLNASP
9   ASPGLULEUSERLEUARGALALEUGLNALA
10   THRGLYGLYASPILEGLNALAALALEUGLU
11   LEUILEPHEALAGLYGLYALAPRO

Samples:

sample_1: HGB1-UBA, [U-13C; U-15N], 1 mM; PBS 200 mM; NaCl 100 mM

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1

Software:

SPARKY -

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

BMRB 15156 15380 16627 16670 16873 16882 17810 18397 5569 7280
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