BMRB Entry 6985

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the human Hexim1 TBD
Deposition date:
2006-02-14
Original release date:
2006-09-07
Authors:
Dames, Sonja; Schoenichen, Andre; Grzesiek, Stephan; Geyer, Matthias
Citation:

Citation: Dames, Sonja; Schoenichen, Andre; Grzesiek, Stephan; Geyer, Matthias. "NMR assignment of the Cyclin T-binding domain of human Hexim 1"  J. Biomol. NMR 36, 39-39 (2006).
PubMed: 16917728

Assembly members:

Assembly members:
Hexim1 TBD, polymer, 107 residues, 12661 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: Novagen

Data sets:
Data typeCount
13C chemical shifts447
15N chemical shifts112
1H chemical shifts740

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11
2subunit 21

Entities:

Entity 1, subunit 1 107 residues - 12661 Da.

cloning artefact: N-terminal GA mutation A4 (= A256 in full length) instead of G

1   GLYALAMETALAGLYASPGLYSERGLUPHE
2   LEUGLNARGASPPHESERGLUTHRTYRGLU
3   ARGTYRHISTHRGLUSERLEUGLNASNMET
4   SERLYSGLNGLULEUILELYSGLUTYRLEU
5   GLULEUGLULYSCYSLEUSERARGMETGLU
6   ASPGLUASNASNARGLEUARGLEUGLUSER
7   LYSARGLEUGLYGLYASPASPALAARGVAL
8   ARGGLULEUGLULEUGLULEUASPARGLEU
9   ARGALAGLUASNLEUGLNLEULEUTHRGLU
10   ASNGLULEUHISARGGLNGLNGLUARGALA
11   PROLEUSERLYSPHEGLYASP

Samples:

sample_1: Hexim1 TBD, [U-90% 15N], 1.2 mM; potassium phosphate 20 mM; sodium chloride 50 mM; TCEP-HCl 10 mM; DTE 1 mM; H2O 95%; D2O 5%

sample_2: Hexim1 TBD, [U-95% 13C; U-90% 15N], 0.6 mM; potassium phosphate 20 mM; sodium chloride 50 mM; TCEP-HCl 10 mM; DTE 1 mM; H2O 95%; D2O 5%

sample_3: Hexim1 TBD, [U-95% 13C; U-90% 15N], 0.6 mM; potassium phosphate 20 mM; sodium chloride 50 mM; TCEP-HCl 10 mM; DTE 1 mM; D2O 100%

sample_4: Hexim1 TBD, [U-10% 13C], 0.6 mM; potassium phosphate 20 mM; sodium chloride 50 mM; TCEP-HCl 10 mM; DTE 1 mM; D2O 100%

sample_5: Hexim1 TBD, [U-95% 13C; U-90% 15N], 1 mM; potassium phosphate 20 mM; sodium chloride 50 mM; TCEP-HCl 10 mM; DTE 1 mM; H2O 95%; D2O 5%

sample_6: Hexim1 TBD, [U-95% 13C; U-90% 15N], 0.6 mM; potassium phosphate 20 mM; sodium chloride 50 mM; TCEP-HCl 10 mM; DTE 1 mM; PF1 phage 16 mg/ml; H2O 95%; D2O 5%

conditions_1: pH: 7.2; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions

Software:

NMRPipe, F. Delaglio - data processing

NMRView v5.0.4, B. Johnson - resonance assignment

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAA36166 BAG36332 BAG51211 BAG74079
GB AAH06460 ABM84198 ABM87157 ABM87602 AIC55820
REF NP_001181605 NP_006451 XP_001488620 XP_002919799 XP_003270766
SP O94992
AlphaFold O94992

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks