BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6972

Title: NMR assignment of the a' domain of thermophilic fungal protein disulfide isomerase   PubMed: 16819589

Authors: Nakano, Michiko; Murakami, Chiho; Yamaguchi, Yoshiki; Sasakawa, Hiroaki; Harada, Takushi; Kurimoto, Eiji; Asami, Osamu; Kajino, Tsutomu; Kato, Koichi

Citation: Nakano, Michiko; Murakami, Chiho; Yamaguchi, Yoshiki; Sasakawa, Hiroaki; Harada, Takushi; Kurimoto, Eiji; Asami, Osamu; Kajino, Tsutomu; Kato, Koichi. "NMR Assignments of the b' and a' Domains of Thermophilic Fungal Protein Disulfide Isomerase"  J. Biomol. NMR 36, 44-44 (2006).

Assembly members:
PDI-a', polymer, 121 residues, Formula weight is not available

Natural source:   Common Name: thermophilic fungus   Taxonomy ID: 34413   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Humicola insolens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
PDI-a': GPLGSEGPVTVVVAKNYNEI VLDDTKDVLIEFYAPWCGHC KALAPKYEELGALYAKSEFK DRVVIAKVDATANDVPDEIQ GFPTIKLYPAGAKGQPVTYS GSRTVEDLIKFIAENGKYKA A

Data sets:
Data typeCount
13C chemical shifts422
15N chemical shifts115
1H chemical shifts769

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDI-a'1

Entities:

Entity 1, PDI-a' 121 residues - Formula weight is not available

1   GLYPROLEUGLYSERGLUGLYPROVALTHR
2   VALVALVALALALYSASNTYRASNGLUILE
3   VALLEUASPASPTHRLYSASPVALLEUILE
4   GLUPHETYRALAPROTRPCYSGLYHISCYS
5   LYSALALEUALAPROLYSTYRGLUGLULEU
6   GLYALALEUTYRALALYSSERGLUPHELYS
7   ASPARGVALVALILEALALYSVALASPALA
8   THRALAASNASPVALPROASPGLUILEGLN
9   GLYPHEPROTHRILELYSLEUTYRPROALA
10   GLYALALYSGLYGLNPROVALTHRTYRSER
11   GLYSERARGTHRVALGLUASPLEUILELYS
12   PHEILEALAGLUASNGLYLYSTYRLYSALA
13   ALA

Samples:

sample_1: PDI-a', [U-13C; U-15N], 1 mM

conditions_1: pH: 6.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableconditions_1
1H13C HSQCsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
CBCANHsample_1not availableconditions_1
HBHA(CO)NHsample_1not availableconditions_1
15N-edited TOCSYsample_1not availableconditions_1
H(CC)(CO)NHsample_1not availableconditions_1
C(CO)NHsample_1not availableconditions_1
HCCH-COSYsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
1H-1H TOCSYsample_1not availableconditions_1
1H-1H NOESYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker Avance 600 MHz
  • Bruker DMX 500 MHz

Related Database Links:

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