BMRB Entry 6963

Title:
1H Chemical Shift Assignments for the Second Kunitz domain of human WFIKKN1, Pro61 in cis- or trans- conformation
Deposition date:
2006-01-30
Original release date:
2006-06-28
Authors:
Liepinsh, Edvards; Otting, Gottfried
Citation:

Citation: Liepinsh, Edvards; Nagy, Alinda; Trexler, Maria; Patthy, Laszlo; Otting, Gottfried. "Second Kunitz-type protease inhibitor domain of the human WFIKKN1 protein"  J. Biomol. NMR 35, 73-78 (2006).
PubMed: 16791741

Assembly members:

Assembly members:
second Kunitz domain, polymer, 70 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts820

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Second Kunitz domain of human WFIKKN1, cis-conformer1
2Second Kunitz domain of human WFIKKN1, trans-conformer1

Entities:

Entity 1, Second Kunitz domain of human WFIKKN1, cis-conformer 70 residues - Formula weight is not available

Asp8 in our numbering corresponds to Asp157 in the GB entry AF422194. Val62 in our numbering corresponds to Val411 in the GB entry AF422194. The first 7 and last 8 residues of our construct were not part of the natural amino acid sequence.

1   GLUALAGLUALAGLUPHETHRASPALACYS
2   VALLEUPROALAVALGLNGLYPROCYSARG
3   GLYTRPGLUPROARGTRPALATYRSERPRO
4   LEULEUGLNGLNCYSHISPROPHEVALTYR
5   GLYGLYCYSGLUGLYASNGLYASNASNPHE
6   HISSERARGGLUSERCYSGLUASPALACYS
7   PROVALVALASPHISHISHISHISHISHIS

Samples:

sample_1: second Kunitz domain, none, 1.1 ± 0.1 mM

conditions_1: pH: 4.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1not availableconditions_1
TOCSYsample_1not availableconditions_1
DQF-COSYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DMX-600 600 MHz
  • Varian Inova 800 800 MHz