BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6957

Title: Solution structure of the SCAN homodimer from MZF-1/ZNF42   PubMed: 16950398

Authors: Volkman, B.; Peterson, F.; Sander, T.; Waltner, J.

Citation: Peterson, F.; Hayes, P.; Waltner, J.; Heisner, A.; Jensen, D.; Sander, T.; Volkman, B.. "Structure of the SCAN domain from the tumor suppressor protein MZF1"  J. Mol. Biol. 363, 137-147 (2006).

Assembly members:
SCAN, polymer, 94 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
SCAN: GSDPGPEAARLRFRCFHYEE ATGPQEALAQLRELCRQWLR PEVRSKEQMLELLVLEQFLG ALPPEIQARVQGQRPGSPEE AAALVDGLRREPGG

Data sets:
Data typeCount
13C chemical shifts398
15N chemical shifts93
1H chemical shifts653

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SCAN subunit 11
2SCAN subunit 21

Entities:

Entity 1, SCAN subunit 1 94 residues - Formula weight is not available

1   GLYSERASPPROGLYPROGLUALAALAARG
2   LEUARGPHEARGCYSPHEHISTYRGLUGLU
3   ALATHRGLYPROGLNGLUALALEUALAGLN
4   LEUARGGLULEUCYSARGGLNTRPLEUARG
5   PROGLUVALARGSERLYSGLUGLNMETLEU
6   GLULEULEUVALLEUGLUGLNPHELEUGLY
7   ALALEUPROPROGLUILEGLNALAARGVAL
8   GLNGLYGLNARGPROGLYSERPROGLUGLU
9   ALAALAALALEUVALASPGLYLEUARGARG
10   GLUPROGLYGLY

Samples:

sample_1: SCAN, [U-15N; U-13C], 2 mM; sodium phosphate 20 mM; NaCl 50 mM; H2O 90%; D2O 10%

sample_2: SCAN, [U-15N; U-13C], 1.7 mM; sodium phosphate 20 mM; NaCl 50 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 99 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablesample_cond_1
3D 13C-separated NOESYnot availablenot availablesample_cond_1
3D 13C-separated NOESY (AROMATIC)not availablenot availablesample_cond_1
3D 13C-F1-filtered-13C-F3-separated NOESYnot availablenot availablesample_cond_1

Software:

X-PLOR NIH v2.9.3. - refinement

xwinnmr v3.5 -

NMRPipe v2004 -

SPSCAN v1.1.0 -

XEASY v1.3 -

GARANT v2.1 -

CYANA v2.1 -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAD92718
GB AAD55809 AAD55810 AAF80465 AAH07777 AAH53316
REF NP_001253962 NP_003413 NP_932172 XP_001099728 XP_001099833
SP P28698