BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6929

Title: 1H, 13C, and 15N Chemical Shift Assignments for Nitrosomonas Europaea Protein Ne2328: Northeast Structural Genomics Consortium target NeT3

Authors: Eletsky, Alexandre; Liu, Gaohua; Yee, Adelinda; Arrowsmith, Cheryl; Szyperski, Thomas

Citation: Eletsky, Alexandre; Liu, Gaohua; Yee, Adelinda; Arrowsmith, Cheryl; Szyperski, Thomas. "Solution NMR Structure of Nitrosomonas Europaea Protein Ne2328"  Proteins: Struct. Funct. Genet. ., .-..

Assembly members:
NeT3, polymer, 107 residues, Formula weight is not available

Natural source:   Common Name: Nitrosomonas europea   Taxonomy ID: 915   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Nitrosomonas europea

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
NeT3: MGTSHHHHHHSSGRENLYFQ GHMNNQVEPRKLVVYGREGC HLCEEMIASLRVLQKKSWFE LEVINIDGNEHLTRLYNDRV PVLFAVNEDKELCHYFLDSD VIGAYLS

Data sets:
Data typeCount
13C chemical shifts342
15N chemical shifts79
1H chemical shifts586

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NeT3 monomer1

Entities:

Entity 1, NeT3 monomer 107 residues - Formula weight is not available

1   METGLYTHRSERHISHISHISHISHISHIS
2   SERSERGLYARGGLUASNLEUTYRPHEGLN
3   GLYHISMETASNASNGLNVALGLUPROARG
4   LYSLEUVALVALTYRGLYARGGLUGLYCYS
5   HISLEUCYSGLUGLUMETILEALASERLEU
6   ARGVALLEUGLNLYSLYSSERTRPPHEGLU
7   LEUGLUVALILEASNILEASPGLYASNGLU
8   HISLEUTHRARGLEUTYRASNASPARGVAL
9   PROVALLEUPHEALAVALASNGLUASPLYS
10   GLULEUCYSHISTYRPHELEUASPSERASP
11   VALILEGLYALATYRLEUSER

Samples:

sample_1: NeT3, [U-95% 13C; U-90% 15N], 0.6 – 1.0 mM; MOPS buffer 10 mM; NaCl 450 mM; DTT 10 mM; benzamidine 1 mM; NaN3 0.01%

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1isotropicconditions_1
1H13C_ct-HSQC_alisample_1isotropicconditions_1
1H13C_ct-HSQC_arosample_1isotropicconditions_1
1H13C_HSQC_alisample_1isotropicconditions_1
1H13C_HSQC_arosample_1isotropicconditions_1
HNCOsample_1not availableconditions_1
GFT (4,3)D HNNCABCAsample_1not availableconditions_1
GFT (4,3)D CABCA(CO)NHNsample_1not availableconditions_1
GFT (4,3)D HABCAB(CO)NHNsample_1not availableconditions_1
GFT (4,3)D HCCHsample_1not availableconditions_1
Simultaneous 13C-, 15N-resolved [1H, 1H] NOESYsample_1not availableconditions_1
Aromatic 13C-resolved [1H, 1H] NOESYsample_1not availableconditions_1

Software:

PROSA v6.0.2, Peter Guentert - data processing

NMRPipe v2.3, Delaglio - data processing

CARA v1.5, Rochus Keller - data analysis

CYANA v2.1, Peter Guentert - structure solution

CNS v1.1, Brunger - structure refinement

VNMR v6.1C, Varian Inc. - data collection

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 750 MHz

Related Database Links:

PDB
EMBL CAD86240
REF NP_842325 WP_011112812