BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6927

Title: Spinophilin PDZ domain   PubMed: 16607467

Authors: Kelker, Matthew; Peti, Wolfgang

Citation: Kelker, Matthew; Peti, Wolfgang. "NMR assignment of the Spinophilin PDZ domain (493-602)"  J. Biomol. NMR 36, 24-24 (2006).

Assembly members:
spinophilin, polymer, 113 residues, 11668 Da.

Natural source:   Common Name: rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
spinophilin: GHMELFPVELEKDSEGLGIS IIGMGAGADMGLEKLGIFVK TVTEGGAAHRDGRIQVNDLL VEVDGTSLVGVTQSFAASVL RNTKGRVRFMIGRERPGEQS EVAQLIQQTLEQE

Data sets:
Data typeCount
13C chemical shifts447
15N chemical shifts110
1H chemical shifts755

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1spinophlin PDZ domain1

Entities:

Entity 1, spinophlin PDZ domain 113 residues - 11668 Da.

1   GLYHISMETGLULEUPHEPROVALGLULEU
2   GLULYSASPSERGLUGLYLEUGLYILESER
3   ILEILEGLYMETGLYALAGLYALAASPMET
4   GLYLEUGLULYSLEUGLYILEPHEVALLYS
5   THRVALTHRGLUGLYGLYALAALAHISARG
6   ASPGLYARGILEGLNVALASNASPLEULEU
7   VALGLUVALASPGLYTHRSERLEUVALGLY
8   VALTHRGLNSERPHEALAALASERVALLEU
9   ARGASNTHRLYSGLYARGVALARGPHEMET
10   ILEGLYARGGLUARGPROGLYGLUGLNSER
11   GLUVALALAGLNLEUILEGLNGLNTHRLEU
12   GLUGLNGLU

Samples:

sample_1: spinophilin 2.0 mM; Na phohphate buffer 20 mM; NaCl 50 mM; NaN3 0.02%

conditions_1: ionic strength: 50 mM; pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCACONHsample_1not availableconditions_1
HCCH_TOCSYsample_1not availableconditions_1
HNCOsample_1not availableconditions_1

Software:

CARA v1.3 - chemical shift assignment

NMR spectrometers:

  • Bruker AvanceII 500 MHz

Related Database Links:

PDB
DBJ BAG51442
EMBL CAC37685 CAD28455
GB AAB72005 AAC05183 AAH16162 AAH29672 AAI60878
REF NP_115984 NP_445926 NP_758465 XP_003131641 XP_003281307
SP O35274 Q6R891 Q96SB3