BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6926

Title: Chemical Shift Assignments of Thylakoid Soluble Phosphoprotein of 9 kDa   PubMed: 17176085

Authors: Song, Jikui; Carlberg, Inger; Lee, Min; Markley, John

Citation: Song, Jikui; Lee, Min; Carlberg, Inger; Vener, A.; Markley, John. "Micelle-induced folding of spinach thylakoid soluble phosphoprotein of 9 kDa and its functional implications"  Biochemistry 45, 15633-15643 (2006).

Assembly members:
TSP9, polymer, 84 residues, Formula weight is not available

Natural source:   Common Name: spinach   Taxonomy ID: 3562   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Spinacia oleracea

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
TSP9: SAAKGTAETKQEKSFVDWLL GKITKEDQFYETDPILRGGD VKSSGSTSGKKGGTTSGKKG TVSIPSKKKNGNGGVFGGLF AKKD

Data sets:
Data typeCount
13C chemical shifts313
15N chemical shifts87
1H chemical shifts403

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1TSP91

Entities:

Entity 1, TSP9 84 residues - Formula weight is not available

1   SERALAALALYSGLYTHRALAGLUTHRLYS
2   GLNGLULYSSERPHEVALASPTRPLEULEU
3   GLYLYSILETHRLYSGLUASPGLNPHETYR
4   GLUTHRASPPROILELEUARGGLYGLYASP
5   VALLYSSERSERGLYSERTHRSERGLYLYS
6   LYSGLYGLYTHRTHRSERGLYLYSLYSGLY
7   THRVALSERILEPROSERLYSLYSLYSASN
8   GLYASNGLYGLYVALPHEGLYGLYLEUPHE
9   ALALYSLYSASP

Samples:

sample_1: TSP9, [U-15N; U-13C], 0.5 ± 0.1 mM; HEPES 10 ± 1 mM; NaCl 100 ± 5 mM; SDS 75 ± 5 mM

conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
1H,13C-HSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCACONHsample_1not availableconditions_1
CCONHsample_1not availableconditions_1
HCCONHsample_1not availableconditions_1
HCCHTOCSYsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HBACONHsample_1not availableconditions_1
3D 15N edited 1H,1H NOESYsample_1not availableconditions_1
3D 13C edited 1H,1H NOESYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

PDB
EMBL CAD45559