BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6920

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Mid1 BBox 1   PubMed: 16529770

Authors: Massiah, Michael; Simmons, Brandi; Short, Keiran; Cox, Tim

Citation: Massiah, Michael; Simmons, Brandi; Short, Keiran; Cox, Tim. "Solution Structure of the RBCC/TRIM B-box1 Domain of Human MID1: B-box with a RING"  J. Mol. Biol. 358, 532-545 (2006).

Assembly members:
B-box 1 type 1, polymer, 78 residues, 8620 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
B-box 1 type 1: QKASVSGPNSPSETRRERAF DANTMTSAEKVLCQFCDQDP AQDAVKTCVTCEVSYCDECL KATHPNKKPFTGHRLIEP

Data sets:
Data typeCount
13C chemical shifts174
15N chemical shifts73
1H chemical shifts421

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Midline-1 B-box Type 11
2ZINC (II) ION, 12
3ZINC (II) ION, 22

Entities:

Entity 1, Midline-1 B-box Type 1 78 residues - 8620 Da.

Sequence corresponds to Gln87 to Pro164 in Midline1

1   GLNLYSALASERVALSERGLYPROASNSER
2   PROSERGLUTHRARGARGGLUARGALAPHE
3   ASPALAASNTHRMETTHRSERALAGLULYS
4   VALLEUCYSGLNPHECYSASPGLNASPPRO
5   ALAGLNASPALAVALLYSTHRCYSVALTHR
6   CYSGLUVALSERTYRCYSASPGLUCYSLEU
7   LYSALATHRHISPROASNLYSLYSPROPHE
8   THRGLYHISARGLEUILEGLUPRO

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: B-box 1 type 1, [U-13C; U-15N], 0.75 ± 0.1 mM; ZINC (II) ION mM; Tris 50 mM; NaCl 300 mM

conditions_1: pH: 7.5; temperature: 294 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCACONHsample_1not availableconditions_1
HMQC-NOE-HSQCsample_1not availableconditions_1
15N-HSQC-NOESYsample_1not availableconditions_1
13C-HSQC-NOESYsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz

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