BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6911

Title: 13C, 15N and 1H assignment of the PDZ1 domain of hMAGI-1 using QUASI   PubMed: 16636753

Deposition date: 2005-12-01 Original release date: 2006-05-02

Authors: Charbonnier, Sebastian; Coutouly, Marie-Aude; Kieffer, Bruno; Trave, Gilles; Atkinson, R.

Citation: Charbonnier, Sebastian; Coutouly, Marie-Aude; Kieffer, Bruno; Trave, Gilles; Atkinson, R.. "13C, 15N and 1H Resonance Assignment of the PDZ1 domain of MAGI-1 using QUASI"  J. Biomol. NMR 36, 33-33 (2006).

Assembly members:
MAGI PDZ1, polymer, 129 residues, 13855.8 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
MAGI PDZ1: GAMGKPFFTRNPSELKGKFI HTKLRKSSRGFGFTVVGGDE PDEFLQIKSLVLDGPAALDG KMETGDVIVSVNDTCVLGHT HAQVVKIFQSIPIGASVDLE LCRGYPLPFDPDDPNTSLVT SVAILDKEP

Data sets:
Data typeCount
13C chemical shifts369
15N chemical shifts104
1H chemical shifts765

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MAGI-1 PDZ11

Entities:

Entity 1, MAGI-1 PDZ1 129 residues - 13855.8 Da.

1   GLYALAMETGLYLYSPROPHEPHETHRARG
2   ASNPROSERGLULEULYSGLYLYSPHEILE
3   HISTHRLYSLEUARGLYSSERSERARGGLY
4   PHEGLYPHETHRVALVALGLYGLYASPGLU
5   PROASPGLUPHELEUGLNILELYSSERLEU
6   VALLEUASPGLYPROALAALALEUASPGLY
7   LYSMETGLUTHRGLYASPVALILEVALSER
8   VALASNASPTHRCYSVALLEUGLYHISTHR
9   HISALAGLNVALVALLYSILEPHEGLNSER
10   ILEPROILEGLYALASERVALASPLEUGLU
11   LEUCYSARGGLYTYRPROLEUPROPHEASP
12   PROASPASPPROASNTHRSERLEUVALTHR
13   SERVALALAILELEUASPLYSGLUPRO

Samples:

sample_1: MAGI-1 PDZ1, [U-100% 13C; U-100% 15N], 0.125 ± 0.01 mM; Tris-HCl 25 ± 0.1 mM; NaCl 50 ± 0.1 mM

conditions_1: pH: 6.8; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
15N-edited_NOESYsample_1not availableconditions_1
15N-edited_TOCSYsample_1not availableconditions_1
1H13C_HSQCsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HN(CO)CACBsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
HCCH-COSYsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
13C-edited_NOESYsample_1not availableconditions_1

Software:

xwinnmr v2.6, Bruker - acquisition

NMRPipe, NIH - processing

CARA, ETH - analysis

QUASI, UMR7104 -

NMR spectrometers:

  • Bruker DRX Avance 600 MHz

Related Database Links:

BMRB 16558 16559
PDB
REF XP_006108887

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts