BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6880

Title: 1H, 13C and 15N chemical shift assignments of Schistocerca gregaria chymotrypsin inhibitor at pH=6.0   PubMed: 16623717

Authors: Gaspari, Zoltan; Szenthe, Borbala; Patthy, Andras; Westler, William; Graf, Laszlo; Perczel, Andras

Citation: Gaspari, Zoltan; Szenthe, Borbala; Patthy, Andras; Westler, William; Graf, Laszlo; Perczel, Andras. "Local binding with globally distributed changes in a small protease inhibitor upon enzyme binding"  FEBS J. 273, 1831-1842 (2006).

Assembly members:
Schistocerca gregaria chymotrypsin inhibitor, polymer, 35 residues, Formula weight is not available

Natural source:   Common Name: desert locust   Taxonomy ID: 7010   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Schistocerca Gregaria

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Schistocerca gregaria chymotrypsin inhibitor: EVTCEPGTTFKDKCNTCRCG SDGKSAACTLKACPQ

Data sets:
Data typeCount
15N chemical shifts23
1H chemical shifts91
T1 relaxation values23
T2 relaxation values23

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1sgci1

Entities:

Entity 1, sgci 35 residues - Formula weight is not available

1   GLUVALTHRCYSGLUPROGLYTHRTHRPHE
2   LYSASPLYSCYSASNTHRCYSARGCYSGLY
3   SERASPGLYLYSSERALAALACYSTHRLEU
4   LYSALACYSPROGLN

Samples:

sample_1: Schistocerca gregaria chymotrypsin inhibitor, [U-15N], 1.5 mM

ex-cond_1: pH: 6.0; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
3D TOCSY-HSQCnot availablenot availablenot available
3D NOESY-HSQCnot availablenot availablenot available
15N T1not availablenot availablenot available
15N T2not availablenot availablenot available
1H,15N NOEnot availablenot availablenot available

Software:

XEASY v1.3.6, ETH Zurich - peak assignment

NMR spectrometers:

  • Bruker DRX 500 MHz

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