BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6877

Title: NMR assignments of the Human Papillomavirus type 16 E2C DNA-bound form   PubMed: 16716065

Authors: Eliseo, Tommaso; Nadra, Alejandro; Paci, Maurizio; de Prat-Gay, Gonzalo; Cicero, Daniel

Citation: Cicero, Daniel; Nadra, Alejandro; Eliseo, Tommaso; Dellarole, Mariano; Paci, Maurizio; de Prat-Gay, Gonzalo. "Structural and Thermodynamic Basis for the Enhanced Transcriptional Control by the Human Papillomavirus Strain-16 E2 Protein"  Biochemistry 45, 6551-6560 (2006).

Assembly members:
HPV E2C, polymer, 81 residues, 9366 Da.
site35 DNA, polymer, 18 residues, 5500 Da.

Natural source:   Common Name: Human Papillomavirus type 16   Taxonomy ID: 10566   Superkingdom: Viruses   Kingdom: not available   Genus/species: Papillomavirus Human Papillomavirus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
HPV E2C: MTPIVHLKGDANTLKCLRYR FKKHCTLYTAVSSTWHWTGH NVKHKSAIVTLTYDSEWQRD QFLSQVKIPKTITVSTGFMS I
site35 DNA: GTAACCGAAATCGGTTGA

Data sets:
Data typeCount
13C chemical shifts245
15N chemical shifts76
1H chemical shifts343

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HPV E2C chain A1
2HPV E2C chain B1
3site 35 strand A2
4site 35 strand B2

Entities:

Entity 1, HPV E2C chain A 81 residues - 9366 Da.

1   METTHRPROILEVALHISLEULYSGLYASP
2   ALAASNTHRLEULYSCYSLEUARGTYRARG
3   PHELYSLYSHISCYSTHRLEUTYRTHRALA
4   VALSERSERTHRTRPHISTRPTHRGLYHIS
5   ASNVALLYSHISLYSSERALAILEVALTHR
6   LEUTHRTYRASPSERGLUTRPGLNARGASP
7   GLNPHELEUSERGLNVALLYSILEPROLYS
8   THRILETHRVALSERTHRGLYPHEMETSER
9   ILE

Entity 2, site 35 strand A 18 residues - 5500 Da.

1   DGDTDADADCDCDGDADADA
2   DTDCDGDGDTDTDGDA

Samples:

sample_1: HPV E2C, [U-15N], 0.6 mM; site35, none, 0.7 mM; NaCl 250 mM; MgCl2 5 mM; Sodium Phosphate 10 mM; Sodium Acetate 50 mM; DTT 5 mM

sample_2: HPV E2C, [U-15N; U-13C], 0.6 mM; site35, none, 0.7 mM; Sodium Chloride 250 mM; Magnesium Chloride 5 mM; Sodium Phosphate 10 mM; Sodium Acetate 50 mM; DTT 5 mM

conditions_1: pH: 5.6; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_2not availableconditions_1
HNCOsample_2not availableconditions_1
HNCAsample_2not availableconditions_1
HN(CO)CAsample_2not availableconditions_1
CBCA(CO)NHsample_2not availableconditions_1
15N NOESYsample_2not availableconditions_1
13C NOESYsample_1not availableconditions_1

Software:

NMRPipe - Data processing

NMRView v5.0.4 - Data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 5952
PDB
DBJ BAN63221 BAN63229 BAN63253 BAN63261 BAN63269
GB AAA46941 AAD03824 AAD03825 AAD03826 AAD03827
REF NP_041328
SP P03120