BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6783

Title: Backbone/Sidechain assignments for RKIP.   PubMed: 16456706

Deposition date: 2005-08-18 Original release date: 2006-03-10

Authors: McElheny, Dan; Clark, Matthew; Wojcik, John; Kurutz, Josh; Rosner, Marsha; Koide, Shohei

Citation: Clark, Matthew; McElheny, Dan; Wojcik, John; Kurutz, Josh; Rosner, Marsha; Koide, Shohei. "NMR Assignment of Rat Raf Kinase Inhibitor Protein"  J. Biomol. NMR 36, 4-4 (2006).

Assembly members:
Raf Kinase Inhibitor Protein, polymer, 194 residues, Formula weight is not available

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Raf Kinase Inhibitor Protein: GSPGIRLMAADISQWAGPLS LQEVDEPPQHALRVDYGGVT VDELGKVLTPTQVMNRPSSI SWDGLDPGKLYTLVLTDPDA PSRKDPKFREWHHFLVVNMK GNDISSGTVLSEYVGSGPPK DTGLHRYVWLVYEQEQPLNC DEPILSNKSGDNRGKFKVES FRKKYHLGAPVAGTCFQAEW DDSVPKLHDQLAGK

Data sets:
Data typeCount
13C chemical shifts724
15N chemical shifts170
1H chemical shifts909

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RKIP1

Entities:

Entity 1, RKIP 194 residues - Formula weight is not available

1   GLYSERPROGLYILEARGLEUMETALAALA
2   ASPILESERGLNTRPALAGLYPROLEUSER
3   LEUGLNGLUVALASPGLUPROPROGLNHIS
4   ALALEUARGVALASPTYRGLYGLYVALTHR
5   VALASPGLULEUGLYLYSVALLEUTHRPRO
6   THRGLNVALMETASNARGPROSERSERILE
7   SERTRPASPGLYLEUASPPROGLYLYSLEU
8   TYRTHRLEUVALLEUTHRASPPROASPALA
9   PROSERARGLYSASPPROLYSPHEARGGLU
10   TRPHISHISPHELEUVALVALASNMETLYS
11   GLYASNASPILESERSERGLYTHRVALLEU
12   SERGLUTYRVALGLYSERGLYPROPROLYS
13   ASPTHRGLYLEUHISARGTYRVALTRPLEU
14   VALTYRGLUGLNGLUGLNPROLEUASNCYS
15   ASPGLUPROILELEUSERASNLYSSERGLY
16   ASPASNARGGLYLYSPHELYSVALGLUSER
17   PHEARGLYSLYSTYRHISLEUGLYALAPRO
18   VALALAGLYTHRCYSPHEGLNALAGLUTRP
19   ASPASPSERVALPROLYSLEUHISASPGLN
20   LEUALAGLYLYS

Samples:

sample_1: Raf Kinase Inhibitor Protein, [U-13C; U-15N], 0.7 mM; Tris HCl buffer, [U-2H], 50 mM; NaCl 100 mM; D2O 7%

conditions_1: ionic strength: 120 mM; pH: 7.3; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
C(CCO)NHnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available
HA(CO)NHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

PDB
EMBL CAA50708 CAA53032
GB AAH63171 EDM13831
PRF 2117380A 2207216A
REF NP_058932
SP P31044

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts