BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6782

Title: 1H, 13C, and 15N Chemical Shift Assignments for E. coli protein YbiA

Authors: Ramelot, Theresa; Xiao, Rong; Montelione, Gaetano; Kennedy, Michael

Citation: Ramelot, Theresa; Xiao, Rong; Montelione, Gaetano; Kennedy, Michael. "Solution NMR Structure of E. coli YbiA. Northeast Structural Genomics Target ET24."  The BMRB entry is the only known published source for the data..

Assembly members:
YbiA, polymer, 168 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Eschericia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
YbiA: MPVRAQRIQHVMQDTIINFY STSDDYGDFSNFAAWPIKVD GKTWPTSEHYFQAQKFLDEK YREEIRRVSSPMVAARMGRD RSKPLRKNWESVKEQVMRKA LRAKFEQHAELRALLLATAP AKLVEHTENDAYWGDGGHGK GKNRLGYLLMELREQLAIEK LEHHHHHH

Data sets:
Data typeCount
13C chemical shifts737
15N chemical shifts170
1H chemical shifts1167
coupling constants44

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YbiA1

Entities:

Entity 1, YbiA 168 residues - Formula weight is not available

1   METPROVALARGALAGLNARGILEGLNHIS
2   VALMETGLNASPTHRILEILEASNPHETYR
3   SERTHRSERASPASPTYRGLYASPPHESER
4   ASNPHEALAALATRPPROILELYSVALASP
5   GLYLYSTHRTRPPROTHRSERGLUHISTYR
6   PHEGLNALAGLNLYSPHELEUASPGLULYS
7   TYRARGGLUGLUILEARGARGVALSERSER
8   PROMETVALALAALAARGMETGLYARGASP
9   ARGSERLYSPROLEUARGLYSASNTRPGLU
10   SERVALLYSGLUGLNVALMETARGLYSALA
11   LEUARGALALYSPHEGLUGLNHISALAGLU
12   LEUARGALALEULEULEUALATHRALAPRO
13   ALALYSLEUVALGLUHISTHRGLUASNASP
14   ALATYRTRPGLYASPGLYGLYHISGLYLYS
15   GLYLYSASNARGLEUGLYTYRLEULEUMET
16   GLULEUARGGLUGLNLEUALAILEGLULYS
17   LEUGLUHISHISHISHISHISHIS

Samples:

sample_1: YbiA, [U-15N; U-13C], 1 ± 0.2 mM; MES 20 mM; NaCL 100 mM; DTT 10 mM; NaN3 0.02%; D2O 10%

sample_2: YbiA, [U-15N; U-5% 13C], 1 ± 0.2 mM; MES 20 mM; NaCL 100 mM; DTT 10 mM; NaN3 0.02%; D2O 10%

sample_3: YbiA, [U-15N; U-13C], 1 ± 0.2 mM; MES 20 mM; NaCL 100 mM; DTT 10 mM; NaN3 0.02%; D2O 100%

conditions_1: pH: 6.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 13C NOESY (arom)sample_1isotropicconditions_1
CBCACONHsample_1isotropicconditions_1
CCONHsample_1isotropicconditions_1
CCH-TOCSYsample_1isotropicconditions_1
1H13C HSQC (aliph)sample_1isotropicconditions_1
1H13C HSQC (arom)sample_1isotropicconditions_1
HBCBCGCDCEHEsample_1isotropicconditions_1
HBCBCGCDHDsample_1isotropicconditions_1
HCCONHsample_1isotropicconditions_1
HCCH-COSYsample_1isotropicconditions_1
HCCH-TOCSYsample_1isotropicconditions_1
HNCAsample_1isotropicconditions_1
HNCACBsample_1isotropicconditions_1
HNCOsample_1isotropicconditions_1
HNCOCAsample_1isotropicconditions_1
1H15N HSQC (long)sample_1isotropicconditions_1
1H15N HSQC (NH2 only)sample_1isotropicconditions_1
1H15N HSQC (sw1)sample_1isotropicconditions_1
1H13C HSQC CT (arom)sample_1isotropicconditions_1
3D 13C NOESY (aliph)sample_1isotropicconditions_1
3D 15N NOESYsample_1isotropicconditions_1
1H15N HSQCsample_1isotropicconditions_1
1H13C HSQC (long)sample_2isotropicconditions_1
HNHAsample_2isotropicconditions_1
1H15N HSQCsample_2isotropicconditions_1
1H15N HSQC (15 min)sample_3isotropicconditions_1
1H15N HSQC (22 days)sample_3isotropicconditions_1
3D 13C NOESY (aliph)sample_3isotropicconditions_1
4D 1H13C-1H13C 4D noesysample_3isotropicconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Unity Inova 600 MHz
  • Varian Unity Inova 750 MHz
  • Varian Unity Inova 800 MHz

Related Database Links:

PDB
DBJ BAA35458 BAG76378 BAI24243 BAI29685 BAI34816
EMBL CAQ31299 CAQ89811 CAQ97701 CAR12151 CAU96708
GB AAA53654 AAC73885 AAZ87525 ABB65362 ABV05209
REF NP_415319 WP_001145123 WP_001145126 WP_001145127 WP_001145128
SP P30176 Q323Z6 Q3Z3Y7