BMRB Entry 6738

Title:
Solution structure of Sep15 from Drosophila melanogaster
Deposition date:
2005-07-21
Original release date:
2005-12-28
Authors:
Ferguson, A.; Labunskyy, V.; Fomenko, D.; Arac, D.; Chelliah, Y.; Amezcua, C.; Rizo, J.; Gladyshev, V.; Deisenhofer, J.
Citation:

Citation: Ferguson, A.; Labunskyy, V.; Fomenko, D.; Arac, D.; Chelliah, Y.; Amezcua, C.; Rizo, J.; Gladyshev, V.; Deisenhofer, J.. "NMR structures of the selenoproteins Sep15 and SelM reveal redox activity of new thioredoxin-like family."  J. Biol. Chem. ., .-. (2005).
PubMed: 16319061

Assembly members:

Assembly members:
Selenoprotein Sep15, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Drosophila melanogaster   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts809
13C chemical shifts496
15N chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Selenoprotein Sep151

Entities:

Entity 1, Selenoprotein Sep15 126 residues - Formula weight is not available

1   METALASERHISHISHISHISHISHISLEU
2   ASPGLNGLNPROALAALAGLNARGTHRTYR
3   ALALYSALAILELEUGLUVALCYSTHRCYS
4   LYSPHEARGALATYRPROGLNILEGLNALA
5   PHEILEGLNSERGLYARGPROALALYSPHE
6   PROASNLEUGLNILELYSTYRVALARGGLY
7   LEUASPPROVALVALLYSLEULEUASPALA
8   SERGLYLYSVALGLNGLUTHRLEUSERILE
9   THRLYSTRPASNTHRASPTHRVALGLUGLU
10   PHEPHEGLUTHRHISLEUALALYSASPGLY
11   ALAGLYLYSASNSERTYRSERVALVALGLU
12   ASPALAASPGLYASPASPASPGLUASPTYR
13   LEUARGTHRASNARGILE

Samples:

sample_1: Selenoprotein Sep15, [U-15N; U-13C], 1 mM; phosphate buffer 50 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6; temperature: 298 K; ionic strength: 50 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablesample_cond_1
3D 13C-separated NOESYsample_1not availablesample_cond_1
2D NOESYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v1, Delaglio - processing

NMRView v5.2.2, Johnson - data analysis

ARIA v2.0, Nilges - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

REF XP_002094982 XP_002076553 XP_002030922 NP_649000
GenBank EDX15740 EDW94694 EDW41908 AAM51078 AAF49314
EMBL CAL26591 CAL26585 CAL26581 CAL26580 CAL26579
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks