BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6724

Title: Nuclear Magnetic Resonance Structure-Based Epitope Mapping and Modulation of Dust Mite Group 13 Allergen as a Hypoallergen

Deposition date: 2005-07-03 Original release date: 2006-04-05

Authors: Chan, Siew Leong; Mok, Yu-Keung

Citation: Chan, Siew Leong; Ong, Seow Theng; Ong, Su Yin; Chew, Fook Tim; Mok, Yu Keung. "Nuclear Magnetic Resonance Structure-Based Epitope Mapping and Modulation of Dust Mite Group 13 Allergen as a Hypoallergen"  J. Immunol. 176, 4852-4860 (2006).

Assembly members:
Der f 13, polymer, 131 residues, Formula weight is not available

Natural source:   Common Name: House Dust Mites   Taxonomy ID: 6954   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Dermatophagoides farinae

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Der f 13: MASIEGKYKLEKSEKFDEFL DKLGVGFMVKTAAKTLKPTF EVAIENDQYIFRSLSTFKNT EAKFKLGEEFEEDRADGKRV KTVIQKEGDNKFVQTQFGDK EVKIIREFNGDEVVVTASCD GVTSVRTYKRI

Data sets:
Data typeCount
13C chemical shifts397
15N chemical shifts130
1H chemical shifts741

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Fatty_acid binding protein1

Entities:

Entity 1, Fatty_acid binding protein 131 residues - Formula weight is not available

1   METALASERILEGLUGLYLYSTYRLYSLEU
2   GLULYSSERGLULYSPHEASPGLUPHELEU
3   ASPLYSLEUGLYVALGLYPHEMETVALLYS
4   THRALAALALYSTHRLEULYSPROTHRPHE
5   GLUVALALAILEGLUASNASPGLNTYRILE
6   PHEARGSERLEUSERTHRPHELYSASNTHR
7   GLUALALYSPHELYSLEUGLYGLUGLUPHE
8   GLUGLUASPARGALAASPGLYLYSARGVAL
9   LYSTHRVALILEGLNLYSGLUGLYASPASN
10   LYSPHEVALGLNTHRGLNPHEGLYASPLYS
11   GLUVALLYSILEILEARGGLUPHEASNGLY
12   ASPGLUVALVALVALTHRALASERCYSASP
13   GLYVALTHRSERVALARGTHRTYRLYSARG
14   ILE

Samples:

sample_1: Der f 13, [U-15N], 1 ± 0 mM; acetate buffer 50 mM; D2O 10%

sample_2: Der f 13, [U-13C], 1 ± 0 mM; acetate buffer 50 mM; D2O 10%

sample_3: Der f 13, [U-13C; U-15N], 1 ± 0 mM; acetate buffer 50 mM; D2O 10%

conditions_1: pH: 4.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HCCH-TOCSYsample_2not availableconditions_1
HCCCONHsample_3not availableconditions_1
CBCACONHsample_3not availableconditions_1
HNCACBsample_3not availableconditions_1
1H15N_HSQCsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
GB AAP35078 AIO08854

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts