BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6723

Title: NMR structure of the heme chaperone CcmE reveals a novel functional motif   PubMed: 12429096

Deposition date: 2005-07-01 Original release date: 2005-10-14

Authors: Enggist, Elisabeth; Thony-Meyer, Linda; Guntert, Peter; Pervushin, Konstantin

Citation: Enggist, Elisabeth; Thony-Meyer, Linda; Guntert, Peter; Pervushin, Konstantin. "NMR structure of the heme chaperone CcmE reveals a novel functional motif"  Structure (Cambridge, MA, U. S.) 10, 1551-1557 (2002).

Assembly members:
Escherichia coli apo-CcmE, polymer, 136 residues, 15325 Da.

Natural source:   Common Name: Escherichia coli   Taxonomy ID: not available   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Escherichia coli apo-CcmE: LRSNIDLFYTPGEILYGKRE TQQMPEVGQRLRVGGMVMPG SVQRDPNSLKVTFTIYDAEG SVDVSYEGILPDLFREGQGV VVQGELEKGNHILAKEVLAK HDENYTPPEVEKAMEANHRR PASVYKDPASHHHHHH

Data sets:
Data typeCount
1H chemical shifts877
13C chemical shifts369
15N chemical shifts126

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apo-CcmE1

Entities:

Entity 1, apo-CcmE 136 residues - 15325 Da.

1   LEUARGSERASNILEASPLEUPHETYRTHR
2   PROGLYGLUILELEUTYRGLYLYSARGGLU
3   THRGLNGLNMETPROGLUVALGLYGLNARG
4   LEUARGVALGLYGLYMETVALMETPROGLY
5   SERVALGLNARGASPPROASNSERLEULYS
6   VALTHRPHETHRILETYRASPALAGLUGLY
7   SERVALASPVALSERTYRGLUGLYILELEU
8   PROASPLEUPHEARGGLUGLYGLNGLYVAL
9   VALVALGLNGLYGLULEUGLULYSGLYASN
10   HISILELEUALALYSGLUVALLEUALALYS
11   HISASPGLUASNTYRTHRPROPROGLUVAL
12   GLULYSALAMETGLUALAASNHISARGARG
13   PROALASERVALTYRLYSASPPROALASER
14   HISHISHISHISHISHIS

Samples:

sample_1: Escherichia coli apo-CcmE, [U-98% 13C; U-98% 15N], 1.1 mM; sodium phosphate 50 mM; sodium chloride 300 mM

Condition_1: pH: 7.2; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D [15N,1H]-TROSYsample_1not availableCondition_1
3D TROSY HNCAsample_1not availableCondition_1
HNCACBsample_1not availableCondition_1
CBCA(CO)NHsample_1not availableCondition_1
HCCH-TOCSYsample_1not availableCondition_1
15N-edited TOCSYsample_1not availableCondition_1
15N-edited NOESY (tau = 60 ms)sample_1not availableCondition_1
13C-edited NOESY (tau = 60 ms)sample_1not availableCondition_1
13Caromatic-edited NOESY (tau = 60 ms)sample_1not availableCondition_1
2D NOESY (tau = 60 ms)sample_1not availableCondition_1
2D TOCSYsample_1not availableCondition_1
1D variant of [15N,13C]-double-half-filter experimentsample_1not availableCondition_1

Software:

xEASY v1.3.13 - data analysis

PROSA - data processing

CANDID - automated NOESY cross peaks assignment

DYANA -

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAB36509 BAE76660 BAG77989 BAI26335 BAI31443
EMBL CAP76699 CAQ32600 CAQ89788 CAQ99124 CAR03625
GB AAA16389 AAC75257 AAG57332 AAN43800 AAN81188
REF NP_288777 NP_311113 NP_416701 NP_708093 NP_754620
SP A1AD51 A7ZP19 A8A259 B1IY75 B1LKU3

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts