BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6712

Title: Solution structure of the two N-terminal CCP modules of C4b-binding protein (C4BP) alpha-chain   PubMed: 16330538

Deposition date: 2005-06-30 Original release date: 2007-02-05

Authors: Jenkins, Huw; Mark, Linda; Ball, Graeme; Lindahl, Gunnar; Uhrin, Dusan; Blom, Anna; Barlow, Paul

Citation: Jenkins, Huw; Mark, Linda; Ball, Graeme; Persson, Jenny; Lindahl, Gunnar; Uhrin, Dusan; Blom, Anna; Barlow, Paul. "Human C4b-binding protein, structural basis for interaction with Streptococcal M protein, a major bacterial virulence factor"  J. Biol. Chem. 281, 3690-3697 (2006).

Assembly members:
C4BP12, polymer, 133 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
C4BP12: MNCGPPPTLSFAAPMDITLT ETRFKTGTTLKYTCLPGYVR SHSTQTLTCNSDGEWVYNTF CIYKRCRHPGELRNGQVEIK TDLSFGSQIEFSCSEGFFLI GSTTSRCEVQDRGVGWSHPL PQCEILEHHHHHH

Data sets:
Data typeCount
13C chemical shifts546
15N chemical shifts132
1H chemical shifts848

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1C4BP121

Entities:

Entity 1, C4BP12 133 residues - Formula weight is not available

1   METASNCYSGLYPROPROPROTHRLEUSER
2   PHEALAALAPROMETASPILETHRLEUTHR
3   GLUTHRARGPHELYSTHRGLYTHRTHRLEU
4   LYSTYRTHRCYSLEUPROGLYTYRVALARG
5   SERHISSERTHRGLNTHRLEUTHRCYSASN
6   SERASPGLYGLUTRPVALTYRASNTHRPHE
7   CYSILETYRLYSARGCYSARGHISPROGLY
8   GLULEUARGASNGLYGLNVALGLUILELYS
9   THRASPLEUSERPHEGLYSERGLNILEGLU
10   PHESERCYSSERGLUGLYPHEPHELEUILE
11   GLYSERTHRTHRSERARGCYSGLUVALGLN
12   ASPARGGLYVALGLYTRPSERHISPROLEU
13   PROGLNCYSGLUILELEUGLUHISHISHIS
14   HISHISHIS

Samples:

sample_1: C4BP12, [U-13C; U-15N], 2.5 mM

conditions_1: pH: 4.5; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 15N-HSQCnot availablenot availablenot available
2D 13C-HSQCnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D CBCANHnot availablenot availablenot available
3D HBHA(CO)NHnot availablenot availablenot available
3D HBHANHnot availablenot availablenot available
3D H(CC)CONHnot availablenot availablenot available
3D (H)C(CC)ONHnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HNCACOnot availablenot availablenot available
3D HCCH-TOCSYnot availablenot availablenot available
2D CG(CB)Hnot availablenot availablenot available
2D CG(CD)Hnot availablenot availablenot available
2D CG(Caro)H-TOCSYnot availablenot availablenot available
3D 13C-NOESYnot availablenot availablenot available
3D 15N-NOESYnot availablenot availablenot available
3D 15N-TOCSYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker AVANCE 800 MHz
  • Bruker AVANCE 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts