BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6696

Title: G83N TPR domain of Ppp5   PubMed: 16531226

Authors: Cliff, Matthew; Harris, Richard; Barford, David; Ladbury, John; Williams, Mark

Citation: Cliff, Matthew; Harris, Richard; Barford, David; Ladbury, John; Williams, Mark. "Conformational Diversity in the TPR Domain-Mediated Interaction of Protein Phosphatase 5 with Hsp90"  Structure 14, 415-426 (2006).

Assembly members:
tetratricopeptide domain from Ppp5, polymer, 140 residues, 16179 Da.
tetratricopeptide domain from Ppp5, polymer, 6 residues, 621 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
tetratricopeptide domain from Ppp5: MSGHHHHHHTDPPADGALKR AEELKTQANDYFKAKDYENA IKFYSQAIELNPSNAIYYGN RSLAYLRTECYGYALNDATR AIELDKKYIKGYYRRAASNM ALGKFRAALRDYETVVKVKP HDKDAKMKYQECNKIVKQKA
tetratricopeptide domain from Ppp5: XMEEVD

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts563
15N chemical shifts138
1H chemical shifts933

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PP5-TPR1
2Hsp90 Pentapeptide2

Entities:

Entity 1, PP5-TPR 140 residues - 16179 Da.

Residues 19 - 147 of Ppp5 with G83N mutation and N-terminal His-tag.

1   METSERGLYHISHISHISHISHISHISTHR
2   ASPPROPROALAASPGLYALALEULYSARG
3   ALAGLUGLULEULYSTHRGLNALAASNASP
4   TYRPHELYSALALYSASPTYRGLUASNALA
5   ILELYSPHETYRSERGLNALAILEGLULEU
6   ASNPROSERASNALAILETYRTYRGLYASN
7   ARGSERLEUALATYRLEUARGTHRGLUCYS
8   TYRGLYTYRALALEUASNASPALATHRARG
9   ALAILEGLULEUASPLYSLYSTYRILELYS
10   GLYTYRTYRARGARGALAALASERASNMET
11   ALALEUGLYLYSPHEARGALAALALEUARG
12   ASPTYRGLUTHRVALVALLYSVALLYSPRO
13   HISASPLYSASPALALYSMETLYSTYRGLN
14   GLUCYSASNLYSILEVALLYSGLNLYSALA

Entity 2, Hsp90 Pentapeptide 6 residues - 621 Da.

1   ACEMETGLUGLUVALASP

Samples:

sample_1: tetratricopeptide domain from Ppp5, [U-13C; U-15N], 0.75 ± 0.4 mM; Hsp90 pentapeptide 0.75 ± 0.4 mM; MES 50 ± 0.2 mM; DTT 5 ± 0.1 mM

conditions_1: ionic strength: 56 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15NHSQCsample_1not availableconditions_1
1H13CHSQCsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
CBCACONHsample_1not availableconditions_1
HNHBsample_1not availableconditions_1
1H15NTOWNY HSQCsample_1not availableconditions_1
1H15NNOESY HSQCsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
1H13CNOESY HSQCsample_1not availableconditions_1
HCCH-TOCSY-HSQCsample_1not availableconditions_1

Software:

ANSIG v3.1 -

NMRPipe -

NMR spectrometers:

  • Varian Unity Inova 800 MHz
  • Varian Unity Inova 600 MHz
  • Varian Unity Plus 500 MHz

Related Database Links:

PDB
DBJ BAD96942 BAE90444 BAG35563 BAI45682
EMBL CAA61595 CAH91828
GB AAB60384 AAD22669 AAH00750 AAH01831 AAH01970
REF NP_001127468 NP_001179178 NP_006238 XP_001111749 XP_001167704
SP P53041