BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6644

Title: 1H, 13C, and 15N NMR assignments for AlgH, a putative transcriptional regulator from Pseudomonas aeruginosa   PubMed: 16222564

Authors: Cowley, Aaron; Bieber Urbauer, Ramona; Urbauer, Jeffrey

Citation: Cowley, Aaron; Bieber Urbauer, Ramona; Urbauer, Jeffrey. "1H, 13C, and 15N NMR Assignments for AlgH, a Putative Transcriptional Regulator from Pseudomonas Aeruginosa"  J. Biomol. NMR 33, 74-74 (2005).

Assembly members:
AlgH, polymer, 189 residues, 21339.08 Da.

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
AlgH: MKQSSPTYLKHHFLIAMPHM ADPNFAQTVTYLVEHNEQGA MGLVINRPSGLNLAEVLEQL KPDALPPARCQHIDIYNGGP VQTDRGFVLHPSGLSYQSTL ELGELAMSTSQDVLFAIAAG TGPEKSLISLGYAGWEAGQL EAELSDNAWLTCPADPAILF DLPPEERLSAAAARLGVNLS LLTAQAGHA

Data sets:
Data typeCount
13C chemical shifts804
15N chemical shifts193
1H chemical shifts1282

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1AlgH1

Entities:

Entity 1, AlgH 189 residues - 21339.08 Da.

1   METLYSGLNSERSERPROTHRTYRLEULYS
2   HISHISPHELEUILEALAMETPROHISMET
3   ALAASPPROASNPHEALAGLNTHRVALTHR
4   TYRLEUVALGLUHISASNGLUGLNGLYALA
5   METGLYLEUVALILEASNARGPROSERGLY
6   LEUASNLEUALAGLUVALLEUGLUGLNLEU
7   LYSPROASPALALEUPROPROALAARGCYS
8   GLNHISILEASPILETYRASNGLYGLYPRO
9   VALGLNTHRASPARGGLYPHEVALLEUHIS
10   PROSERGLYLEUSERTYRGLNSERTHRLEU
11   GLULEUGLYGLULEUALAMETSERTHRSER
12   GLNASPVALLEUPHEALAILEALAALAGLY
13   THRGLYPROGLULYSSERLEUILESERLEU
14   GLYTYRALAGLYTRPGLUALAGLYGLNLEU
15   GLUALAGLULEUSERASPASNALATRPLEU
16   THRCYSPROALAASPPROALAILELEUPHE
17   ASPLEUPROPROGLUGLUARGLEUSERALA
18   ALAALAALAARGLEUGLYVALASNLEUSER
19   LEULEUTHRALAGLNALAGLYHISALA

Samples:

sample_1: AlgH, [U-99% 13C; U-99% 15N], 1.3 mM; potassium phosphate 10 mM; KCl 10 mM; DTT 5 mM; D2O 8%; H2O 92%; Sodium azide 0.02%

conditions_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available
H(CCO)NH TOCSYnot availablenot availablenot available
C(CO)NH TOCSYnot availablenot availablenot available
1H13C HSQCnot availablenot availablenot available
(HB)CB(CGCD)HDnot availablenot availablenot available

Software:

FELIX v2000 -

NMR spectrometers:

  • Varian INOVA 600 MHz

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