BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6624

Title: Backbone 1H, 13C, and 15N Resonance Assignments for the 13kD Ras Association Domain 1 (RA1) from Phospholipase C (epsilon)   PubMed: 16258837

Authors: Harris, Richard; Bunney, Tom; Katan, Matilda; Driscoll, Paul

Citation: Harris, Richard; Bunney, Tom; Katan, Matilda; Driscoll, Paul. "Backbone 1H, 13C, and 15N Resonance Assignments for the two 13 kD Ras Associating Domains (RA1 and RA2) from Phospholipase C(epsilon)"  J. Biomol. NMR 33, 138-138 (2005).

Assembly members:
ra1, polymer, 110 residues, 12788.6 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
ra1: GEERKCLQTHRVTVHGVPGP EPFTVFTINGGTKAKQLLQQ ILTNEQDIKPVTTDYFLMEE KYFISKEKNECRKQPFQRAI GPEEEIMQILSSWFPEEGYM GRIVLKTQQE

Data sets:
Data typeCount
13C chemical shifts362
15N chemical shifts92
1H chemical shifts674

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RA11

Entities:

Entity 1, RA1 110 residues - 12788.6 Da.

additional Glycine at N-terminus

1   GLYGLUGLUARGLYSCYSLEUGLNTHRHIS
2   ARGVALTHRVALHISGLYVALPROGLYPRO
3   GLUPROPHETHRVALPHETHRILEASNGLY
4   GLYTHRLYSALALYSGLNLEULEUGLNGLN
5   ILELEUTHRASNGLUGLNASPILELYSPRO
6   VALTHRTHRASPTYRPHELEUMETGLUGLU
7   LYSTYRPHEILESERLYSGLULYSASNGLU
8   CYSARGLYSGLNPROPHEGLNARGALAILE
9   GLYPROGLUGLUGLUILEMETGLNILELEU
10   SERSERTRPPHEPROGLUGLUGLYTYRMET
11   GLYARGILEVALLEULYSTHRGLNGLNGLU

Samples:

15N: ra1, [U-15N], 1 mM; sodium chloride 250 mM; DTT 5 mM; TRIS 25 mM; EDTA 0.1 mM

13C15N: ra1, [U-13C; U-15N], 1 mM; sodium chloride 250 mM; DTT 5 mM; TRIS 25 mM; EDTA 0.1 mM

13C: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCnot availablenot available13C
13C1H HSQCnot availablenot available13C
HNCAnot availablenot available13C
HNcoCAnot availablenot available13C
HNCACBnot availablenot available13C
CBCAcoNHnot availablenot available13C
HNCOnot availablenot available13C
HAcacoNHnot availablenot available13C
HCCH-TOCSYnot availablenot available13C
13C NOESYnot availablenot available13C
15N NOESYnot availablenot available13C

Software:

ANSIG v3.3 - analysing NMR spectra

NMRPipe v2.3 - Processing NMR spectra

AZARA v2.7 - conversion of nmrPipe format data to ansig format

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian UnityPlus 500 MHz

Related Database Links:

PDB
DBJ BAA96040 BAB14090 BAG10469
GB AAF22005 AAG17145 AAG28341 AAI40706 AAI44287
REF NP_001159451 NP_001275918 NP_057425 XP_003825424 XP_003825425
SP Q9P212